We narrowed to 2,094 results for: PAM

1. pAAV-Syn_CapChR2_mScarlet

   Plasmid

   #188039

   Purpose

   Expresses CapChR2-mScarlet in mammalian neurons

   Depositor

   Peter Hegemann

   Article

   Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

   Insert

   CapChR2-mScarlet
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mutation

   S43D, L112C, T139C and N238E mutations in CoChR

   Promoter

   hSyn

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA-D2s-L-Venus

   Plasmid

   #19966

   Depositor

   Jonathan Javitch

   Article

   Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

   Insert

   D2 short variant-monomeric Venus (DRD2 Human, Aequorea Victoria)
   

   Use

   Tags

   Flag and monomeric Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. EGFP-INPP5E

   Plasmid

   #223564

   Purpose

   Expresses the full-length mouse INPP5E (Pharbin)

   Depositor

   Pietro De Camilli

   Article

   Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

   Insert

   INPP5E (Inpp5e Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDONR223-VPS35

   Plasmid

   #21689

   Depositor

   Lynda Chin

   Article

   Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

   Insert

   Vacuolar Protein Sorting 35 homolog (VPS35 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   last nucleotide of stop codon removed for c-termi…

   Promoter

   Available since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pLV.PGK.mLmx1a

   Plasmid

   #33013

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   LIM homeobox transcription factor 1 alpha (Lmx1a Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Ef1a-DIO-VMAT2-WPRE-pA

   Plasmid

   #39339

   Depositor

   Bernardo Sabatini

   Article

   Tritsch et al Nature. 2012 Oct 11;490(7419):262-6. doi

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV; R

   Tags

   Expression

   Mutation

   Myc tag inserted in the first lumenal loop betwee…

   Promoter

   Available since

   Oct. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Lmx1b

   Plasmid

   #34997

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   LIM homeobox transcription factor 1-beta (Lmx1b Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HA-HIF2α P531A-pBabe-puro

   Plasmid

   #26091

   Depositor

   William Kaelin

   Article

   Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA with point mutation P531A

   Promoter

   Available since

   Sept. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-nLight3.1

   Plasmid

   #111061

   Purpose

   Expresses nLight3.1 in mammalian cells

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Insert

   nLight3.1
   

   Use

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA-D2s-L-V1

    Plasmid

    #19967

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-N terminal Venus (DRD2 Human, Aequorea Victoria)
    

    Use

    Tags

    Flag and N terminal Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-hSyn-eGFP-SynaptoZip

    Plasmid

    #122525

    Purpose

    AAV expression of the synaptic activity-marker SynaptoZip fused to eGFP driven by human Synapsin promoter

    Depositor

    Antonio Malgaroli

    Article

    Ferro et al Nat Commun. 2017 Oct 31;8(1):1229. doi:

    Insert

    eGFP-SynaptoZip (Vamp2 Rat)
    

    Use

    AAV

    Tags

    Myc and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRK5 HA-GST-Presc-mAkt1 (wt)

    Plasmid

    #48805

    Purpose

    expresses wt Akt1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Tags

    HA-GST-Presc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-dLight1.3a

    Plasmid

    #111055

    Purpose

    Expresses dLight1.3a in mammalian cells

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Insert

    dLight1.3a
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA

    Plasmid

    #184633

    Purpose

    Expresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Aldh1a1 (Aldh1a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-hPGK-mCherry-SynaptoZip

    Plasmid

    #177317

    Purpose

    Lentiviral expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by hPGK promoter

    Depositor

    Antonio Malgaroli

    Article

    Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

    Insert

    mCherry-SynaptoZip (Vamp2 Rat)
    

    Use

    Lentiviral

    Tags

    Myc and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-HIF2α MT-pBabe-Hygro

    Plasmid

    #22134

    Depositor

    William Kaelin

    Article

    Kondo et al PLoS Biol. 2003 Dec . 1(3):E83.

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA "silent"("conservative"…

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PL752-CLSTN2-LA-p2a-tdTomato-hGHpA-PGK-puroR-CLSTN2-RA

    Plasmid

    #191685

    Purpose

    For knocking p2a-tdTomato into CLSTN2 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    CLSTN2 homology arms and p2a-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA-D2s-L-V2

    Plasmid

    #19968

    Depositor

    Jonathan Javitch

    Article

    Guo et al EMBO J. 2008 Sep 3. 27(17):2293-304.

    Insert

    D2 short variant-C terminal Venus (DRD2 Human, Aequorea Victoria)
    

    Use

    Tags

    C terminal Venus and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

19. PL752-OTX2-LA-p2a-EGFPnls-hGHpA-PGK-puroR-OTX2-RA

    Plasmid

    #191690

    Purpose

    For knocking p2a-EGFP into OTX2 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    OTX2 homology arms and p2a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PL752-LMX1A-LA-p2a-tdTomato-hGHpA-PGK-puroR-LMX1A-RA

    Plasmid

    #191683

    Purpose

    For knocking p2a-tdTomato into LMX1A locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    LMX1A homology arms and p2a-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pKM60

    Plasmid

    #154159

    Purpose

    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    4 wt NF-κB sites were replaced with Rel1A-specifi…

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLJM60-S6K1(T389S) barcoded

    Plasmid

    #48800

    Purpose

    expresses mutant S6K1

    Depositor

    David Sabatini

    Article

    Kang et al Science. 2013 Jul 26;341(6144):1236566.

    Insert

    S6K1 T389S (Rps6kb1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG and barcode: GGTACC

    Expression

    Mammalian

    Mutation

    T389S

    Promoter

    CMV

    Available since

    Dec. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pAAV-Syn_CapChR1_eYFP

    Plasmid

    #188033

    Purpose

    Expresses CapChR1-eYFP in mammalian neurons

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR1-eYFP
    

    Use

    AAV

    Tags

    eYFP

    Expression

    Mutation

    S63D, L132C, T159C and N258E mutations in CrChR2

    Promoter

    hSyn

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PL452-EN1-LA-p2a-mNeonGreen-hGHpA-PGK-neoR-EN1-RA

    Plasmid

    #191684

    Purpose

    For knocking p2a-mNeonGreen into EN1 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    EN1 homology arms and p2a-mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PL452-CLSTN2-LA-p2a-EGFP-hGHpA-PGK-neoR-CLSTN2-RA

    Plasmid

    #191688

    Purpose

    For knocking p2a-EFGP into CLSTN2 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    CLSTN2 homology arms and p2a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAA3

    Plasmid

    #154163

    Purpose

    Firefly luciferase under the control of 5 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    20 wt NF-κB sites were replaced with Rel1A-specif…

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-Syn_CapChR1_mScarlet

    Plasmid

    #188037

    Purpose

    Expresses CapChR1-mScarlet in mammalian neurons

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR1-mScarlet
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    S63D, L132C, T159C and N258E mutations in CrChR2

    Promoter

    hSyn

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PL752-PTPRO-LA-p2a-tdTomato-hGHpA-PGK-puroR-PTPRO-RA

    Plasmid

    #191686

    Purpose

    For knocking p2a-tdTomato into PTPRO locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    PTPRO homology arms and p2a-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PL452-FGF8-LA-p2a-tdTomato-hGHpA-PGK-neoR-FGF8-RA

    Plasmid

    #191687

    Purpose

    For knocking p2a-tdTomato into FGF8 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    FGF8 homology arms and p2a-tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PL452-PTPRO-LA-p2a-EGFP-hGHpA-PGK-neoR-PTPRO-RA

    Plasmid

    #191689

    Purpose

    For knocking p2a-EGFP into PTPRO locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    PTPRO homology arms and p2a-EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pKM5

    Plasmid

    #154166

    Purpose

    Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel2 (long isoform)
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKM28

    Plasmid

    #154165

    Purpose

    Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel1A
    

    Use

    Tags

    FLAG

    Expression

    Insect

    Mutation

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAA2

    Plasmid

    #154162

    Purpose

    Firefly luciferase under the control of 4 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    16 wt NF-κB sites were replaced with Rel1A-specif…

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAA1

    Plasmid

    #154161

    Purpose

    Firefly luciferase under the control of 3 copies of: the enhancer for the Drosophila melanogaster Metchnikowin gene, 4 Rel1A-specific NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    12 wt NF-κB sites were replaced with Rel1A-specif…

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKM62

    Plasmid

    #154160

    Purpose

    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, with 4 Rel2-specific (VII) NF-_B sites, 3 GATA sites and 3 putative ecdysone-response elements, plus an additional NF-_B site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    4 wt NF-κB sites were replaced with Rel2-specific…

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKM59

    Plasmid

    #154158

    Purpose

    Firefly luciferase under the control of the enhancer for the Drosophila melanogaster Metchnikowin gene, a TATA box and Kozak sequence of the Metchnikowin gene and additional NF-κB site.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Enhancer for the Drosophila melanogaster Metchnik…

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKM52

    Plasmid

    #154155

    Purpose

    Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel2 NF-κB site

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Drosophila melanogaster minimal Hsp70 promoter wi…

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKM51

    Plasmid

    #154154

    Purpose

    Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB site

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Drosophila melanogaster minimal Hsp70 promoter wi…

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. PiggyBac-TetO-Ascl1-neomycin

    Plasmid

    #176482

    Purpose

    TetO-Ascl1 in a PiggyBac vector with neomycin resistance

    Depositor

    Marius Wernig

    Article

    Ng et al Stem Cell Reports. 2021 Jul 13;16(7):176

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

    Plasmid

    #184635

    Purpose

    Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCWori cytochrome P450-BM3h 9D7

    Plasmid

    #61308

    Purpose

    Expresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tag

    Depositor

    Alan Jasanoff

    Article

    Brustad et al J Mol Biol. 2012 Sep 14;422(2):245-62. d

    Insert

    Cytochrome P450-BM3 heme domain 9D7
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    T268A, I263A, T438V, A328G

    Promoter

    Tac

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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43. pLV.PGK.mFoxa2

    Plasmid

    #33014

    Depositor

    Malin Parmar

    Article

    Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

    Insert

    Forkhead box A2 (Foxa2 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    H10L

    Promoter

    Available since

    Jan. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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44. PL752-EN1-LA-p2a-mTagBFP2nls-3xHAtag-hGHpA-PGK-puroR-EN1-RA

    Plasmid

    #191691

    Purpose

    For knocking p2a-mTagBFP2nls-3xHAtag into EN1 locus

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Insert

    EN1 homology arms and p2a-mTagBFP2nls-3xHAtag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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45. pAAV-pTH-iCre:EGFP-WPREpA

    Plasmid

    #213142

    Purpose

    Truncated rat TH promoter expressing iCre fused to EGFP

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAAV-DIO-EF1α-mKate2-biPAC

    Plasmid

    #169127

    Purpose

    AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Insert

    mKate2-biPAC
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    codon optimized to mouse

    Promoter

    EF1a

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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47. AAV-CAG-SPOTlight-U2GCR

    Plasmid

    #164819

    Purpose

    Reporter to measure ISR state-dependent protein synthesis

    Depositor

    Nicole Calakos

    Article

    Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

    Insert

    SPOTlight-U2GCR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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48. pmCherry-Synaptojanin-1-145

    Plasmid

    #223563

    Purpose

    Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

    Depositor

    Pietro De Camilli

    Article

    Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    SYNJ1 (SYNJ1 Human)
    

    Use

    Tags

    His and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pAAV-pTH-iCre-WPREpA

    Plasmid

    #213130

    Purpose

    Truncated rat TH promoter expressing iCre

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV and Mouse Targeting

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available since

    Feb. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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50. pRK5-HA-GST-4EBP1-Low GC

    Plasmid

    #69018

    Purpose

    expresses tagged 4EBP1

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    EIF4EBP1 (EIF4EBP1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Mammalian

    Mutation

    lowGC content, wt is ~70% GC rich. Still, it code…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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