19,646 results

1. lentiCas9n(D10A)-Blast

   Plasmid

   #63593

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, nickase mutant

   Promoter

   EFS-NS

   Available Since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 HA CFP hNKCC1 WT (NT15-H)

   Plasmid

   #49077

   Purpose

   Expresses human NKCC1 with an N-terminal 3xHA-CFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites. Native hNKCC1 amino acid sequence

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x HA and mCerulean

   Expression

   Mammalian

   Mutation

   262 silent mutations from native hNKCC1

   Promoter

   CMV

   Available Since

   Nov. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. mApple-C1

   Plasmid

   #54631

   Purpose

   Localization: C1 Cloning Vector, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Article

   Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

   Type

   Empty backbone

   Tags

   mApple

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas9

   Plasmid

   #167547

   Purpose

   Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.

   Depositor

   Howard Hang

   Article

   Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00

   Inserts

   chloramphenicol acetyl transferase
   pUC19 origin of replication
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT18mobsacB

   Plasmid

   #72648

   Purpose

   sucrose counter-selection allelic exchange vector for Pseudomonas syringae

   Depositor

   Brian Kvitko

   Type

   Empty backbone

   Use

   Sucrose counter-selection allelic exchange vector

   Available Since

   Feb. 18, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. DT960

   Plasmid

   #80412

   Purpose

   Expresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

   Depositor

   Thomas Cooper

   Article

   Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

   Insert

   human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE 80L Kan T7WT

   Plasmid

   #102790

   Purpose

   Expresses T7 RNA polymerase

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Insert

   T7 RNA polymerase
   

   Expression

   Bacterial

   Available Since

   July 3, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. hMEF2A WT

   Plasmid

   #118354

   Purpose

   This plasmid expresses human MEF2A-1 WT.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hMEF2A (MEF2A Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC0042-PguCas13b crRNA backbone

   Plasmid

   #103853

   Purpose

   For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-PguCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Ubi:Zebrabow

    Plasmid

    #118219

    Purpose

    For ubiquitous expression of Brainbow in zebrafish

    Depositor

    Albert Pan, Alex Schier

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV5 BRGI-Flag

    Plasmid

    #19143

    Depositor

    Joan Massague

    Article

    Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-APOBEC1-YTH

    Plasmid

    #131636

    Purpose

    Expresses APOBEC1 fused to the YTH domain

    Depositor

    Kate Meyer

    Article

    Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

    Insert

    APOBEC1 fused to YTH domain (YTHDF2 Rat, Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR GFP-CaaX

    Plasmid

    #113020

    Purpose

    for lentivirus production of GFP-CaaX

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    GFP-CaaX
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-HA-NONO

    Plasmid

    #127655

    Purpose

    Over-express HA-tagged human NONO

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

    Insert

    NONO (NONO Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C2_SEPT9 i4

    Plasmid

    #71617

    Purpose

    mammalian expression of human SEPT9 i4 with a GFP fusion

    Depositor

    Cristina Montagna, Elias Spiliotis

    Article

    Connolly et al Breast Cancer Res. 2011 Aug 10;13(4):R76

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. OKSIM

    Plasmid

    #24603

    Depositor

    Jose Cibelli

    Article

    Ross et al Stem Cells Dev. 2010 Aug;19(8):1221-9

    Insert

    Oct4 KLF4 Sox2 c-Myc (SOX2, POU5F1, MYC, KLF4 Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Mutation

    Oct4-2A-KLF4-2A-Sox2-IRES-Myc

    Available Since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-2E2-HA-mCh

    Plasmid

    #129596

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mCherry. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mCherry (2E2-HA scFv-mCherry)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. GPR161-Tango

    Plasmid

    #66334

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR161 (GPR161 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCMV p16 INK4A

    Plasmid

    #10916

    Depositor

    Bob Weinberg

    Article

    Medema et al Proc Natl Acad Sci U S A. 1995 Jul 3. 92

    Insert

    p16 INK4 (CDKN2A Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSP2-96-merTetO-EFS-BLaR

    Plasmid

    #118713

    Purpose

    Contains an optimized 96-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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