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Showing: 10441 - 10480 of 171429 results

  3. DD-Cas9 with filler sequence and Venus (EDCPV)

    Plasmid
    #90085
    Purpose
    This plasmid contains destabilized Cas9 and has Venus after P2A sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90085-plasmid-map-sequence-id-182691

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  21. qTAG-C-Solo-mStayGold-TOMM20

    Plasmid
    #227307
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227307-plasmid-map-sequence-id-451546

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  25. Ultra-Exon1Q145-Myc-A

    Plasmid
    #110487
    Purpose
    Lentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)
    Depositor
    Baoji Xu
    Article
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Partial long 3'UTR wild type huntingtin exon…
    Promoter
    UbC
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110487-plasmid-map-sequence-id-212608

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  26. Ultra-Exon1Q23-Myc-A

    Plasmid
    #110486
    Purpose
    Lentiviral vector to express wild-type N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged wild type huntingtin exon1 fragment with 23 repeats of Q)
    Depositor
    Baoji Xu
    Article
    Xu et al PLoS One. 2017 May 11;12(5):e0177610. do
    Insert
    Homo sapiens huntingtin (HTT Human)
    Use
    Lentiviral
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Short 3'UTR wild type huntingtin exon1 fragm…
    Promoter
    UbC
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    110486-plasmid-map-sequence-id-212584

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  30. pCW57.1_SPTLC1_C133W

    Plasmid
    #181921
    Purpose
    3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133W
    Depositor
    Christian Metallo
    Article
    Cordes et al J Lipid Res. 2022 Sep 14:100281. doi: 10
    Insert
    Human serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
    Use
    Lentiviral; Mammalian expression, , doxycycline i…
    Expression
    Mammalian
    Mutation
    cysteine at position 133 of the coding region of …
    Promoter
    tight TRE promoter
    Available Since
    May 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    181921-plasmid-map-sequence-id-361675

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  31. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

    Plasmid
    #90086
    Purpose
    This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA
    Depositor
    Raffaella Sordella
    Article
    Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10
    Insert
    Cas9
    Use
    CRISPR and Lentiviral
    Tags
    Destabilized Domain and Flag
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    90086-plasmid-map-sequence-id-182687

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  32. pCHC036 (∆pHG1)

    Plasmid
    #226206
    Purpose
    Conjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.
    Depositor
    Christopher Johnson
    Article
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for the pHG1 megaplasmid replication region
    Use
    Synthetic Biology
    Mutation
    ∆helD ∆repA ∆repB ∆parAB
    Available Since
    Nov. 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226206-plasmid-map-sequence-id-452437

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Showing: 10441 - 10480 of 171429 results