159,371 results

1. pEMB52/14-3-3_1_247

   Plasmid

   #40541

   Depositor

   Michael J Fox Foundation MJFF

   Insert

   YWHAG (YWHAG Human)
   

   Use

   Baculovirus

   Tags

   6xHis

   Expression

   Mutation

   WT; aa1-247

   Promoter

   polH

   Available since

   Oct. 1, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pGEX SH3(2)-4R

   Plasmid

   #112090

   Purpose

   Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   SH3(2)-4R (NCK1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDrVSP-IRES2-EGFP

   Plasmid

   #80333

   Purpose

   Expresses Danio rerio VSP with separated EGFP in mammalian cell

   Depositor

   Yasushi Okamura

   Article

   Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

   Insert

   Daino rerio voltage-sensing phosphatase (tpte Zebrafish)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PYL1_nTEVp

   Plasmid

   #119213

   Purpose

   Expresses N-terminus of tobacco etch virus protease (TEVp) fused to PYL1-abscisic acid responsive protein domain

   Depositor

   Roman Jerala

   Article

   Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

   Insert

   split nTEVp in sufion with PYL1
   

   Use

   Tags

   AU1 tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Twinkle-APEX2-V5

   Plasmid

   #129705

   Purpose

   APEX2 fused to the mitochondrial nucleoid protein Twinkle

   Depositor

   Alice Ting

   Article

   Han et al Cell Chem Biol. 2017 Mar 16;24(3):404-41

   Insert

   Twinkle-APEX2-V5 (TWNK, APEX2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mPA-GFP-Calnexin-N-14

   Plasmid

   #57122

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Insert

   Calnexin (CANX Human)
   

   Use

   Tags

   mPA-GFP

   Expression

   Mammalian

   Mutation

   I474N in Calnexin

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pCS2-3xFLAG-NLS-SpCas9-NLS

   Plasmid

   #51307

   Purpose

   Cas9 expression plasmid

   Depositor

   Yonglong Chen

   Article

   Guo et al Development. 2014 Jan 8.

   Insert

   3xFLAG-NLS-SpCas9-NLS
   

   Use

   CRISPR; Xenopus tropicalis expression

   Tags

   3x Flag and NLS

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC gRNA Shuttle

   Plasmid

   #47024

   Purpose

   Encodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.

   Depositor

   Wayne Parrott

   Article

   Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

   Insert

   gRNA Shuttle
   

   Use

   CRISPR; Cas9

   Tags

   Expression

   Plant

   Mutation

   G to T cloning mutation at position 323

   Promoter

   Medicago truncatula U6.6

   Available since

   Sept. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_Gal4UAS_humanIL-10_IRES_mCherry_PGK_tBFP

   Plasmid

   #85427

   Purpose

   Lentiviral vector for Gal4 inducible humanIL-10 with mCherry reporter and constitutive tBFP expression

   Depositor

   Wendell Lim

   Article

   Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

   Insert

   human IL-10
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PB-GFP11x7-HygR

    Plasmid

    #223676

    Purpose

    To check the split GFP signals in mitochondria (2)

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Nature. 2024 May;629(8012):660-668. doi:

    Insert

    GFP11x7
    

    Use

    Tags

    GFP11

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti.CAG.H2B-Dendra2.W

    Plasmid

    #51005

    Purpose

    Lentivector that expresses H2B-Dendra2 from internal CAG enhancer-promoter

    Depositor

    Rusty Lansford

    Insert

    H2B-Dendra2
    

    Use

    Lentiviral

    Tags

    histone2B

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFGAMS-EGFP

    Plasmid

    #99107

    Purpose

    Expresses human PFAS-EGFP fusion in mammalian cells

    Depositor

    Stephen Benkovic

    Article

    An et al Science. 2008 Apr 4;320(5872):103-6. doi

    Insert

    PFAS (PFAS Human)
    

    Use

    Tags

    EGFP (enhanced green fluorescent protein)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3 HA 14-3-3 sigma

    Plasmid

    #11946

    Depositor

    Michael Yaffe

    Article

    Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

    Insert

    Stratifin (SFN Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GFP-HA-BACE1

    Plasmid

    #165032

    Purpose

    HA-BACE1

    Depositor

    Juan Bonifacino

    Article

    Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

    Insert

    BACE1 (BACE1 Human)
    

    Use

    Tags

    HA (embedded after propeptide cleavage site)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ga13_2-mTq2

    Plasmid

    #112933

    Purpose

    Human Galpha13 tagged with mTurquoise, inserted at residue 128

    Depositor

    Dorus Gadella

    Article

    Mastop et al PLoS One. 2018 Mar 5;13(3):e0193705. doi

    Insert

    Galpha13
    

    Use

    Tags

    mTurquoise2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. FUGW-d2GFP-ZEB1 3'UTR

    Plasmid

    #79601

    Purpose

    FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.

    Depositor

    Jeffrey Rosen

    Article

    Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

    Insert

    d2GFP-Zeb1 3'UTR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiCRISPR-E

    Plasmid

    #78852

    Purpose

    Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

    Depositor

    Phillip Abbosh

    Article

    Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Cas9-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mPA-GFP-Farnesyl-5

    Plasmid

    #57133

    Purpose

    Localization: Membrane, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pSLQ7556 pHR (hU6-crCD46-EFS-PuroR-WPRE)

    Plasmid

    #214880

    Purpose

    Lentiviral vector encoding RfxCas13d targeting CD46 guide

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crCD46-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AiP14870 - pAAV-AiE0452h_3xC2-minBG-ChR2(H134R)-EYFP-WPRE3-BGHpA (Alias: CN4870)

    Plasmid

    #224186

    Purpose

    AiE0452h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D2 MSNs

    Depositor

    Jonathan Ting, The Allen Institute for Brain Science

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MAC_N_ETV4

    Plasmid

    #167753

    Purpose

    Expresses ETV4 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    ETV4 (ETV4 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-sACE2(WT)-Fc(IgG1)

    Plasmid

    #145163

    Purpose

    Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Fc region of human IgG1

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ABI_cTEVp

    Plasmid

    #119214

    Purpose

    Expresses C-terminus of tobacco etch virus protease (TEVp) fused to ABI-abscisic acid responsive protein domain

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    split cTEVp in fusion with ABI
    

    Use

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TRPC3-Myc

    Plasmid

    #25902

    Depositor

    Craig Montell

    Article

    Li et al Neuron. 1999 Sep . 24(1):261-73.

    Insert

    TRPC3 (Trpc3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCrlM

    Plasmid

    #122444

    Purpose

    Expresses myc tagged mouse reelin ORF in mammalian cells

    Depositor

    Tom Curran

    Article

    D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

    Insert

    reelin (Reln Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEntry_SARS-CoV-2-Nucleocapsid

    Plasmid

    #168886

    Purpose

    Gateway-compatible Entry vector

    Depositor

    Pascal Falter-Braun

    Article

    Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

    Insert

    SARS-CoV-2-Nucleocapsid (N )
    

    Use

    Gateway-compatible entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA6-N-Flag-SREBP2

    Plasmid

    #134284

    Purpose

    Expresses Flag-tagged SREBP2 in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

    Insert

    SREBP2 (Srebf2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    mouse SREBP2 precursor

    Promoter

    CMV

    Available since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mu-GM-CSF-pCIneo

    Plasmid

    #163518

    Purpose

    Expresses murine GM-CSF in mammalian cells

    Depositor

    Thomas Weber

    Insert

    murine GM-CSF
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. phGluc

    Plasmid

    #22522

    Depositor

    Christopher Buck

    Article

    Pastrana et al PLoS Pathog. 2009 Sep . 5(9):e1000578.

    Insert

    Gaussia luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMSCV-IRES-GFP-MD2-HA

    Plasmid

    #133065

    Purpose

    Retroviral expression of mouse MD2 tagged with HA

    Depositor

    Jonathan Kagan

    Article

    Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

    Insert

    MD2 (Ly96 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBOB-VE-cadherin-GFP

    Plasmid

    #118732

    Purpose

    To express VE-cadherin tagged with GFP on C-terminus

    Depositor

    John Cooper

    Article

    Kim et al Mol Biol Cell. 2018 Jul 15;29(13):1693-1

    Insert

    Cadherin 5 (CD144) (CDH5 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    I503T (Common SNP-see comments)

    Promoter

    CMV

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCMV-Myc CDC4 WT*

    Plasmid

    #16652

    Depositor

    Bert Vogelstein

    Article

    Rajagopalan et al Nature. 2004 Mar 4. 428(6978):77-81.

    Insert

    CDC4 (FBXW7 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    *Note that this insert is only WT relative to the…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUltra-Chili-Luc

    Plasmid

    #48688

    Purpose

    3rd generation Lentiviral vector with bi-cistronic expression of dtomato and luciferase

    Depositor

    Malcolm Moore

    Article

    Fujiwara et al Mol Cancer Ther. 2021 Jun 4. pii: 1535-7

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFBOH-LIC

    Plasmid

    #26099

    Purpose

    SGC Empty baculovirus expression/transfer vector

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Baculovirus expression

    Tags

    6x His and Thrombin protease cleavage site

    Expression

    Mutation

    Promoter

    polyhedrin

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pTlpA36-Wasabi

    Plasmid

    #86115

    Purpose

    mWasabi reporter gene gated by TlpA36

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA36
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D341A, P60L, D135V, K187R, K202I, L208Q

    Promoter

    pTlpA and pTlpA, LacI

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pX330-FOXL2-cterm

    Plasmid

    #192893

    Purpose

    Expresses Cas9 and sgRNA targeting the C-terminus region of FOXL2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    FOXL2 C-term sgRNA (FOXL2 Synthetic)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pT3TS-nCas9n

    Plasmid

    #46757

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T3

    Available since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PA-mCherry1-H2B-C-10

    Plasmid

    #57181

    Purpose

    Localization: Histones/Nucleus, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    PA-mCherry1

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pB PUb-integrase

    Plasmid

    #183966

    Purpose

    piggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent marker

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    PhiC31 integrase
    

    Use

    Piggybac insect transgenesis destination vector

    Tags

    Expression

    Mutation

    Promoter

    Aedes aegypti Polyubiquitin

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1 FLAG-YY1

    Plasmid

    #104396

    Purpose

    FLAG-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    YY1
    

    Use

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge