We narrowed to 5,482 results for: pcas

1. pDONR207-MUM2sp-ADPG2

   Plasmid

   #170723

   Purpose

   Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)

   Depositor

   George Haughn

   Article

   McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629

   Insert

   ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
   

   Use

   Gateway donor vector / entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)

   Plasmid

   #160617

   Purpose

   TU for the regulated expression of the PhiC31 phage recombination directionality factor (RDF) gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   RDF
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   mini35s

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3 alpha2 P35S:MS2-VP64:Tnos (GB1395)

   Plasmid

   #160609

   Purpose

   TU for the constitutive expresion of the viral coat protein MS2 fused on C-terminal with the VP64

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35S:MS2-VP64:Tnos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)

   Plasmid

   #160614

   Purpose

   TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   mini35s

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)

   Plasmid

   #160615

   Purpose

   TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   mini35s

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. BE3-YE1

   Plasmid

   #132943

   Purpose

   Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

   Depositor

   Hui Yang

   Article

   Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

   Insert

   BE3-(W90Y-R126E)
   

   Use

   CRISPR; Base editor

   Tags

   Expression

   Mammalian

   Mutation

   BE3-(W90Y-R126E)

   Promoter

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330_gRNA_H19-DMR-2

   Plasmid

   #159930

   Purpose

   Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse H19-DMR (H19-icr Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330_gRNA_H19-DMR-1

   Plasmid

   #159929

   Purpose

   Specific gRNA against mouse H19-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of H19-DMR in mouse ESC.

   Depositor

   Anton Wutz

   Article

   Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

   Insert

   gRNA mouse H19-DMR (H19-icr Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, CBh

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT175_GalL_A3A(Y130F)Δ186-BE3

   Plasmid

   #145124

   Purpose

   Expressing base editor A3A(Y130F)Δ186-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A(Y130F)Δ186-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   A3A(Y130F; 1-186AA); spCas9(D10A)

   Promoter

   GalL

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTBL405 CHYRON1 integration construct

    Plasmid

    #126442

    Purpose

    To integrate the CHYRON1 locus at HEK293site3.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON1 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. mei-S332-GFP 5.6kb genomic DNA in Casper4 T331D

    Plasmid

    #112986

    Purpose

    P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331D) into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

    Insert

    mei-S332 (mei-S332 Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Expression

    Mutation

    T331D

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. mei-S332-GFP 5.6kb genomic DNA in Casper4 T331A

    Plasmid

    #112988

    Purpose

    P element vector for transposon insertion of mei-S332-GFP 5.6kb genomic DNA (T331A) into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Nogueira et al G3 (Bethesda). 2014 Jul 31;4(10):1849-58

    Insert

    mei-S332 (mei-S332 Fly)
    

    Use

    P insertion vector

    Tags

    GFP

    Expression

    Mutation

    T331A

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. JG693: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X3)

    Plasmid

    #104570

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to three DmrA domains

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1(D832A)-DmrA(x3)
    

    Use

    Tags

    3x FLAG, 3x HA, and NLS

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. MST1R gRNA (BRDN0001487060)

    Plasmid

    #78050

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MST1R

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MST1R (MST1R Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. MST1R gRNA (BRDN0001145791)

    Plasmid

    #78051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MST1R

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MST1R (MST1R Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. MST1R gRNA (BRDN0001145169)

    Plasmid

    #78052

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human MST1R

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    MST1R (MST1R Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

    Plasmid

    #107270

    Purpose

    Expresses Cas9 upon induction with doxicycline.

    Depositor

    Pablo Menéndez

    Article

    Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

    Inserts

    hSpCas9
    rtTA
    bGH polyA-SV40polyA
    

    Use

    Tags

    3xFlag, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and TRE

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVS1-tet-iCas9-BFP2

    Plasmid

    #125519

    Purpose

    all-in-one-inducible Cas9

    Depositor

    Jason Moffat

    Article

    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

    Insert

    AAVS1-TET-ON 3G-SpCas9-P2A-BFP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1-PDi-CRISPRn

    Plasmid

    #73500

    Purpose

    Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    Cas9
    rtTA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and TRE

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

    Plasmid

    #217662

    Purpose

    expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

    Depositor

    Daniel Gerlich

    Article

    Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

    Insert

    sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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