163,606 results

1. pET-Neq2X7

   Plasmid

   #182366

   Purpose

   Expresses Neq2X7 DNA polymerase

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al BMC Biotechnol. 2024 Apr 2;24(1):17. doi

   Insert

   Neq2X7 DNA polymerase
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-CDT2

   Plasmid

   #215091

   Purpose

   Expresses FLAG-CDT2 in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDT2 (DTL Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-ER GFP

   Plasmid

   #80069

   Purpose

   To express and target GFP into the endoplasmic reticulum using lentivirus

   Depositor

   Pantelis Tsoulfas

   Insert

   ER GFP (SEC61B Human)
   

   Use

   Lentiviral

   Tags

   GFP derived from Aequorea coerulescens on the NH2…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. NM9-SpCas9-NLS3

   Plasmid

   #128177

   Purpose

   Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

   Depositor

   Huimin Zhao

   Article

   Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

   Insert

   SpCas9-NLS3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pPGK1

   Available Since

   April 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-C-6His-mEsrra

   Plasmid

   #173152

   Purpose

   mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells

   Depositor

   Brijesh Kumar Singh

   Article

   Tripathi et al bioRxiv 2021.11.16.468891

   Insert

   ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Wild type

   Promoter

   Available Since

   Aug. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-29b(+)_His-CBD-SpEndoH

   Plasmid

   #218196

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

   Depositor

   Florence Pojer

   Insert

   EndoH
   

   Use

   Tags

   tufA-His-CBD

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 45-313

   Promoter

   T7

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-At-TRL

   Plasmid

   #32242

   Depositor

   Stan Fields, Jay Hesselberth

   Article

   Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

   Insert

   A. thaliana tRNA ligase (RNL Mustard Weed)
   

   Use

   Tags

   6xHistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNA Polmyerase

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. HBM-Luc

   Plasmid

   #35155

   Depositor

   Linda Penn

   Article

   Facchini et al Mol Cell Biol. 1997 Jan;17(1):100-14.

   Insert

   Myc promoter (MYC Human)
   

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   Contains 2.5 kb of the c-myc minimal promoter

   Promoter

   c-myc

   Available Since

   Oct. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. slc1a3b:myrGFP-P2A-H2AmCherry

   Plasmid

   #170208

   Purpose

   Drives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleus

   Depositor

   Kelly Monk

   Article

   Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

   Insert

   slc1a3b (slc1a3b Zebrafish)
   

   Use

   Zebrafish

   Tags

   myrGFP-P2A-H2AmCherry

   Expression

   Mutation

   Promoter

   slc1a3b

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. plix403-V5-N-mCherry

    Plasmid

    #223956

    Purpose

    Gateway cloning of genes of interest with an N-terminal V5-mCherry fusion; generation of stable cells with doxycycline inducible expression

    Depositor

    Karla Feijs, Roko Žaja

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    V5-mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CW3SL-EGFP

    Plasmid

    #61463

    Purpose

    AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmEmerald-ERGIC-53

    Plasmid

    #170717

    Purpose

    Expresses mEmerald-tagged human ERGIC-53 at high level in mammalian cells.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Insert

    ERGIC-53 (LMAN1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCasKP-apr

    Plasmid

    #117231

    Purpose

    Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae

    Depositor

    Quanjiang Ji

    Article

    Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-FLAG-HA-hYTHDC1

    Plasmid

    #85167

    Purpose

    Expression of FLAG-HA-tagged human YTHDC1

    Depositor

    Samie Jaffrey

    Article

    Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Use

    Tags

    Flag and Ha

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRAD1

    Plasmid

    #47697

    Purpose

    General cloning vector for use in E. coli or D. radiodurans

    Depositor

    Mary Lidstrom

    Article

    Meima et al Appl Environ Microbiol. 2000 Sep;66(9):3

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    repU

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-ABCB10-ChR2-eYFP

    Plasmid

    #206037

    Purpose

    Express ChR2 in mitochondria

    Depositor

    Lufang Zhou

    Article

    Ernst et al Biophys J. 2019 Aug 20;117(4):631-645. d

    Insert

    ChR2
    

    Use

    Tags

    ABCB10 mitochondrial leading sequence and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSFV3

    Plasmid

    #92072

    Purpose

    Empty expression plasmid for production of recombinant genomic SFV RNA. Co-transfection with RNA from pSFV-Helper1 or pSFV-Helper2 will produce recombinant virus like particles.

    Depositor

    Henrik Garoff

    Article

    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356

    Type

    Empty backbone

    Use

    Empty expression plasmid, for run-off transcripti…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF0915

    Plasmid

    #143451

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NCOA2 (NCOA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. iYFP-Parkin Plasmid

    Plasmid

    #197107

    Purpose

    Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.

    Depositor

    Dori Woods

    Article

    Capelluto et al Adv Biol (Weinh). 2023 Jan 18:e2200246.

    Insert

    parkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)
    

    Use

    Retroviral

    Tags

    YFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmCherry-N1-EXO1b

    Plasmid

    #111621

    Purpose

    Expression of human WT Exonuclease 1 (EXO1) tagged with mCherry in mammalian cells

    Depositor

    Marco Muzi-Falconi

    Article

    Sertic et al Mol Cell. 2018 Apr 5;70(1):34-47.e4. doi

    Insert

    Exonuclease 1b (EXO1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. TCARZ-SV40-GFP-PAmin-GF3-R4A22

    Plasmid

    #203769

    Purpose

    For production of template RNA for use in PRINT, GFP under SV40 promoter

    Depositor

    Kathleen Collins

    Article

    Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

    Insert

    PRINT template RNA for GFP
    

    Use

    Ivt template

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EBFP2-Nucleus-7

    Plasmid

    #55249

    Purpose

    Localization: Nuclear Localization, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Insert

    SV40 T-Antigen Nuclear Localization Signal (NLS)
    

    Use

    Tags

    EBFP2-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

23. PE-Max-pet21A

    Plasmid

    #204471

    Purpose

    Expression plasmid for PE protein in bacteria.

    Depositor

    Scot Wolfe

    Article

    Ponnienselvan et al Nucleic Acids Res. 2023 May 29:gkad456.

    Insert

    Prime editor
    

    Use

    CRISPR

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-EF1a-iFlpV

    Plasmid

    #140137

    Purpose

    can be used to generate AAV virus that will express light-inducible site-specific iFlpV recombinase

    Depositor

    Hongkui Zeng

    Article

    Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

    Has Service

    AAV PHP.eB and AAV1

    Insert

    iFlpV
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Cas12aUltra_5XNLS

    Plasmid

    #218775

    Purpose

    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

    Depositor

    Ross Wilson

    Article

    Sahu et al bioRxiv 2024.07.14.603391

    Insert

    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    

    Use

    CRISPR

    Tags

    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

    Expression

    Bacterial

    Mutation

    Nucleotides mutations were made to encode for arg…

    Promoter

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLEX307-DPP4-G418

    Plasmid

    #158453

    Purpose

    Constitutive lentiviral expression of human DPP4.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    DPP4 (DPP4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV-CMV-dSa-VPR mini.-syn pA

    Plasmid

    #99689

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9
    

    Use

    AAV

    Tags

    VPR mini

    Expression

    Mutation

    dead Cas9

    Promoter

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCAG-EGFP(HiBiT)

    Plasmid

    #162589

    Purpose

    N-terminally HiBiT-tagged EGFP under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    EGFP
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAG32

    Plasmid

    #35122

    Depositor

    John McCusker

    Article

    Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

    Insert

    hphMX4
    

    Use

    Yeast replicating

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti6/V5-p53_R249S

    Plasmid

    #22935

    Depositor

    Bernard Futscher

    Article

    Junk et al Neoplasia. 2008 May . 10(5):450-61.

    Insert

    tumor protein 53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    R249S Mutant

    Promoter

    Available Since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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