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166,034 results

Showing: 7981 - 8010 of 166034 results

  1. pWALIUM10-V5-TurboID-GW

    Plasmid
    #196285
    Purpose
    Gateway destination vector to express N-terminal TurboID fusion under UAS promoter, for making transgenic flies
    Depositor
    Norbert Perrimon
    Type
    Empty backbone
    Use
    Gateway destination vector
    Expression
    Insect
    Available Since
    March 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    196285-plasmid-map-sequence-id-384638

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  4. KARS_pNIC-Bio3

    Plasmid
    #153051
    Purpose
    Expression construct for producing Avi tagged Lysyl-tRNA synthetase
    Depositor
    Susanne Gräslund
    Insert
    KARS (KARS1 Human)
    Tags
    Avi and His6-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    June 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    153051-plasmid-map-sequence-id-304466

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  7. MCP-3x9aaTAD

    Plasmid
    #210707
    Purpose
    This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module
    Depositor
    Isaac Hilton
    Article
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available Since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    210707-plasmid-map-sequence-id-416252

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  10. pETM6-E12-vioABECD

    Plasmid
    #66537
    Purpose
    pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)
    Depositor
    Mattheos Koffas
    Article
    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10
    Inserts
    VioA
    VioB
    VioE
    VioC
    VioD
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    Mutant T7 Promoter - G6 and Mutant T7 Promoter - …
    Available Since
    July 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    66537-plasmid-map-sequence-id-114568

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  11. pFA6a-HaloTag-KanMX6

    Plasmid
    #87029
    Purpose
    Tag S. pombe genes in C terminal with HaloTag
    Depositor
    Julien Berro
    Insert
    HaloTag
    Expression
    Bacterial and Yeast
    Available Since
    Feb. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87029-plasmid-map-sequence-id-173001

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  14. mEmerald-Calreticulin-N-16

    Plasmid
    #54023
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Calreticulin (CALR Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54023-plasmid-map-sequence-id-474311

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  17. pNL [NlucP/AP1-RE/Hygro] Vector

    Plasmid
    #236811
    Purpose
    Express AP1 response element in luciferase reporter assay
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    AP1
    Use
    Luciferase
    Tags
    NanoLuc (R)
    Mutation
    None
    Promoter
    minP
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236811-plasmid-map-sequence-id-471818

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  18. hNTa2-qgRNA-pYJA5

    Plasmid
    #217780
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Article
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available Since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217780-plasmid-map-sequence-id-469426

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  19. pPonA-BI-Gl NORM-LacZA TER- LacZB

    Plasmid
    #86212
    Purpose
    beta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoter
    Depositor
    Robert Singer
    Article
    Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:
    Insert
    beta-Globin (HBB, Hbb Mouse, Human)
    Use
    Ponasterone a inducible promoter
    Tags
    lacZA or lacZB
    Expression
    Mammalian
    Mutation
    39TER in beta Globin in MCS II
    Promoter
    Ponasterone A inducible
    Available Since
    Jan. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    86212-plasmid-map-sequence-id-169958

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  20. pBG-ttLbUV2

    Plasmid
    #231386
    Purpose
    LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.
    Depositor
    Qi-Jun Chen
    Article
    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:
    Insert
    ttLbCas12a Ultra V2
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D156R and E795L
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    231386-plasmid-map-sequence-id-461803

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  21. pCfB2337

    Plasmid
    #67555
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Available Since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    67555-plasmid-map-sequence-id-116237

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  23. pMAL-c2X_MBP-CLmN-H6

    Plasmid
    #234487
    Purpose
    Maltose binding protein N-terminally fused to His-tagged CLmN split intein
    Depositor
    Henning Mootz
    Article
    Humberg et al Nat Commun. 2025 Mar 19;16(1):2723. doi:
    Insert
    N-terminal Fragment of the CLm Split Intein
    Tags
    Hexahistidine Tag and Maltose Binding Protein (MB…
    Expression
    Bacterial
    Mutation
    T69K, F75H, M118N within the N-terminal fragment …
    Available Since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    234487-plasmid-map-sequence-id-471039

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  25. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A)

    Plasmid
    #188384
    Purpose
    Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor
    Depositor
    Kole Roybal
    Article
    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.
    Insert
    PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
    Use
    Lentiviral
    Available Since
    March 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    188384-plasmid-map-sequence-id-371052

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  26. GPR55-DuET

    Plasmid
    #213285
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GPR55 (GPR55 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213285-plasmid-map-sequence-id-430830

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Showing: 7981 - 8010 of 166034 results