161,727 results

1. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138151

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR_BsmBI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX302 MX1-V5 puro

   Plasmid

   #158640

   Purpose

   Lentiviral expression vector for constitutive expression of V5-tagged MX1.

   Depositor

   Kevin Janes

   Article

   Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

   Insert

   Mx1 (MX1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p-AAV-sh[control]

   Plasmid

   #75438

   Purpose

   control shRNA vector

   Depositor

   Edward Burton

   Article

   Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

   Insert

   GFP
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. XLone-Puro eGFP

   Plasmid

   #140027

   Purpose

   Tunable and temporal expression control of nuclear eGFP

   Depositor

   Xiaoping Bao

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTDH3-dCas9-Mxi1

   Plasmid

   #46921

   Purpose

   Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   dCas9-Mxi1
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. EFNB1-bio-His

   Plasmid

   #51749

   Purpose

   Expresses full-length Ephrin-B1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   EFNB1 (EFNB1 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI-Neo-PTPRT (WT)

   Plasmid

   #16630

   Depositor

   Bert Vogelstein

   Article

   Wang et al Science. 2004 May 21. 304(5674):1164-6.

   Insert

   PTPRT (PTPRT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pMA7CR_2.0

   Plasmid

   #73950

   Purpose

   pMA7CR_2.0 contains arabinose inducible λ/RED β-protein and dam, and aTc inducible CRISPR/Cas9 and recX

   Depositor

   Alex Nielsen

   Article

   Ronda et al Sci Rep. 2016 Jan 22;6:19452. doi: 10.10

   Inserts

   cas9
   lambda beta
   dam
   recX
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pAra and pTet

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. ISceI-GR-RFP

   Plasmid

   #17654

   Depositor

   Tom Misteli

   Article

   Soutoglou et al Nat Cell Biol. 2007 Jun . 9(6):675-82.

   Insert

   I-SceI-GRLBD (SCEI, Nr3c1 Budding Yeast, Rat)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   C655G mutation in the glucocorticoid receptor (se…

   Promoter

   Available since

   March 28, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCE-BiFC-VN173

    Plasmid

    #22019

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Nat Protoc. 2008 . 3(4):588-96.

    Insert

    VN173
    

    Use

    Tags

    Myc

    Expression

    Worm

    Mutation

    N/A

    Promoter

    Available since

    Oct. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tet-O-FUW-EGR3-P2A-mCherry

    Plasmid

    #203861

    Purpose

    Tet-inducible lentiviral plasmid expressing EGR3-P2A-mCherry, with ORF specific barcode close to 3'LTR site

    Depositor

    Andrea Califano

    Article

    Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

    Insert

    EGR3 (EGR3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. GAL4UAS-Luciferase reporter

    Plasmid

    #64125

    Purpose

    Photoactivatable transcription system. Luciferase reporter under the control of the upstream activator sequence (UAS) of Gal4.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    GAL4UAS
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GAL4UAS

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFH2.2_mCherry-MS2x12

    Plasmid

    #205521

    Purpose

    Express mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRRL_U2AF1_S34F_mCherry

    Plasmid

    #84019

    Purpose

    expression of mutant U2AF1 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    U2AF1 (U2AF1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and T2A-mCherry

    Expression

    Mammalian

    Mutation

    S34F

    Promoter

    PGK

    Available since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. Human pIgR(SC)

    Plasmid

    #158216

    Purpose

    Express the ectdomain (SC) of human pIgR

    Depositor

    Junyu Xiao

    Article

    Li et al Science. 2020 Feb 28;367(6481):1014-1017

    Insert

    PIGR (PIGR Human)
    

    Use

    Tags

    8×his

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmr101A-cPr-mNeonGreen (KmR)

    Plasmid

    #177209

    Purpose

    Plasmid with p15A origin for constitutive mNeonGreen expression in E. coli

    Depositor

    Alexey Merz

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Yeast codon optimized

    Promoter

    Synthetic constitutive promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti.PGK.LifeAct-Ruby.W

    Plasmid

    #51009

    Purpose

    Lentivector that expresses LifeAct-Ruby from internal human PGK promoter

    Depositor

    Rusty Lansford

    Insert

    LifeAct-mRFPruby
    

    Use

    Lentiviral

    Tags

    LifeAct

    Expression

    Mammalian

    Mutation

    Promoter

    human PGK

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. PSMC2_GFP_Cterm

    Plasmid

    #221516

    Purpose

    Fluorescently tagged Psmc2-GFP

    Depositor

    Alessandro Ori

    Insert

    PSMC2 (PSMC2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSFFV_sfCherry3V(1-10)

    Plasmid

    #117483

    Purpose

    Lentiviral vector for constitutive expression of sfCherry3V(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    sfCherry3V(1-10)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    6 substitutions (K45R, G52D, L83W, T106A, K182R, …

    Promoter

    SFFV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-GCN5 core

    Plasmid

    #179551

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human GCN5 core (aa 473-676) (KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDB5318

    Plasmid

    #204828

    Purpose

    An integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetS promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. Tol2-mpeg1-dendra2

    Plasmid

    #51462

    Purpose

    expresses photoconvertible dendra in macrophages

    Depositor

    Anna Huttenlocher

    Article

    Harvie et al Infect Immun. 2013 Jan;81(1):110-21. doi

    Insert

    mpeg-dendra
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    mpeg1

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. TUBG1_pLX307

    Plasmid

    #98376

    Purpose

    Lentiviral expression of TUBG1

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    TUBG1 (TUBG1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. 2C::tdTomato Reporter

    Plasmid

    #40281

    Depositor

    Samuel Pfaff

    Article

    Macfarlan et al Nature. 2012 Jul 5;487(7405):57-63.

    Insert

    2C Promoter (2-cell stage promoter)
    

    Use

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG RABV-G

    Plasmid

    #36398

    Depositor

    Connie Cepko

    Article

    Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    rabies virus glycoprotein
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    CAGGs

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pYES1L-URA

    Plasmid

    #84301

    Purpose

    E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

    Depositor

    George Church

    Article

    Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET28a-LIC

    Plasmid

    #26094

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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28. Integrin-β6 I domain I270C

    Plasmid

    #159639

    Purpose

    Contains integrin beta 6 residues 108–352 with I270C mutation

    Depositor

    Timothy Springer

    Article

    Dong et al Nature. 2017 Feb 2;542(7639):55-59. doi:

    Insert

    Integrin beta 6 I domain I270C
    

    Use

    Tags

    6x His

    Expression

    Mammalian

    Mutation

    I270C

    Promoter

    Available since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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29. RfxCas13d-backbone

    Plasmid

    #165078

    Purpose

    Expression of RfxCas13d sgRNAs from a U6 promoter

    Depositor

    Ling-Ling Chen

    Article

    Li et al Nat Methods. 2021 Jan;18(1):51-59. doi:

    Insert

    Scaffold gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. TOP-GFP.mC

    Plasmid

    #35491

    Depositor

    Ramesh Shivdasani

    Article

    Horst et al Cancer Res. 2012 Feb 8.

    Insert

    7xTOP-GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    7xTCF/LEF

    Available since

    May 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge