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FLHKII-pGFPN3

Plasmid

#21920

Depositor

Hossein Ardehali

Article

Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

Insert

Full length human HKII cDNA in pGFP-N3 plasmid (HK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

This is the full length human HKII cDNA sequence …

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

Plasmid

#100834

Purpose

AAV expression of ultrasensitive protein calcium sensor from CamKII promoter

Depositor

James M. Wilson

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

CamKII

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pKM461

Plasmid

#108320

Purpose

For performing ORBIT - Expresses Che9c phage RecT and Bxb1 phage Integrase, and contains SacB

Depositor

Kenan Murphy

Article

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Inserts

Che9C RecT
Bxb1 Integrase
Tet Repressor
SacRB

Expression

Bacterial

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGBKT7-GW

Plasmid

#61703

Purpose

Gateway compatible Y2H vector - BD

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-GFP-BRCA1

Plasmid

#71116

Purpose

mammalian expression of GFP-BRCA1 siRNA resistant construct

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pEvol-pAzFRS.2.t1

Plasmid

#73546

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Inserts

pAzFRS.2.t1
pAzFRS.2.t1

Expression

Bacterial

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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EBFP2-H2B-6

Plasmid

#55243

Purpose

Localization: Nucleus/Histones, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

EBFP2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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eSpCas9(1.1)_No_FLAG_AAVS1_T2

Plasmid

#79888

Purpose

Expresses the AAVS1 T2 gRNA in combination with FLAGless eSpCas9(1.1) to target the AAVS1 "safe harbor" locus.

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Type

Empty backbone

Use

CRISPR

Tags

Untagged eSpCas9(1.1)

Expression

Mammalian

Promoter

CBh

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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TFORF1344

Plasmid

#143792

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FEZF2 (FEZF2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pRK-FLAG-UbxD8

Plasmid

#53777

Purpose

express FLAG-tagged human UbxD8 gene

Depositor

Yihong Ye

Article

Xu et al J Biol Chem. 2013 Jun 21;288(25):18068-7

Insert

UbxD8 (FAF2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCE-K2N

Plasmid

#154879

Purpose

Episomally expresses KLF2 and NANOG in mammalian cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Stem Cell Reports. 2021 Mar 23. pii: S22

Insert

KLF2-2A-NANOG (KLF2, NANOG Human)

Expression

Mammalian

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLX304 GFP

Plasmid

#193687

Purpose

Constitutive lentiviral expression of GFP

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

GFP

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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Stat3 Y705F Flag pRc/CMV

Plasmid

#8709

Depositor

Jim Darnell

Article

Wen et al Nucleic Acids Res 1997 Jun 1;25(11):2062

Insert

Stat3 Y705F (Stat3 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Y705F

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf6

Plasmid

#10834

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 (ARF6 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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mEmerald-Caveolin-C-10

Plasmid

#54025

Purpose

Localization: Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Hanson et al Traffic. 2013 Jun;14(6):663-77. doi: 10.

Insert

Caveolin (CAV1 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
AviTag-SpyCatcher

Plasmid

#72326

Purpose

Bacterial expression of SpyCatcher with a peptide tag enabling site-specific biotinylation.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

AviTag-SpyCatcher

Tags

N-term His6 tag and TEV cleavage site

Expression

Bacterial

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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PGK mScarlet-LC3B

Plasmid

#200083

Purpose

Mammalian expression of fluorescently-tagged marker for autophagic vesicles

Depositor

Erika Holzbaur

Article

Dou et al Cell Rep. 2023 May 1;42(5):112448. doi:

Insert

Map1lc3b (Map1lc3b Rat)

Tags

mScarlet

Expression

Mammalian

Promoter

PGK

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-SauriABE8e

Plasmid

#189927

Purpose

Plasmid encoding SauriABE8e driven by the CMV promoter for plasmid transfection.

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SauriABE8e

Expression

Mammalian

Mutation

Nme2Cas9 D16A

Promoter

CMV

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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ER-mCherry-eMagA

Plasmid

#162248

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

CytP450 ER targeting sequencel-mCherry-eMagA

Expression

Mammalian

Promoter

beta-actin promoter

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TAG-ERP2

Plasmid

#80479

Purpose

All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and puromycin resistance)

Depositor

Knut Woltjen

Article

Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pMIR-FLAG-MLL-AF9

Plasmid

#71444

Purpose

Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, followed by IRES-dsRed Express2

Depositor

Daisuke Nakada

Article

Saito et al Cell Stem Cell. 2015 Sep 29. pii: S1934-

Insert

MLL-AF9 (KMT2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pIB184-Km

Plasmid

#90195

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

Depositor

Indranil Biswas

Article

Hossain et al J Bacteriol. 2012 Jul;194(13):3486-94. d

Type

Empty backbone

Tags

None

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

mCherry

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-GFP-TurboID-NES_pLX304

Plasmid

#202019

Purpose

expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

Flag-GFP-TurboID-NES

Use

Lentiviral

Tags

Flag and GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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H3K9me3 biosensor

Plasmid

#120802

Purpose

FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Truncated YPet-HP1-EV linker-ECFP-mouse histone H3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-YAPS127A

Plasmid

#166458

Purpose

Expresses fusion of mEGFP and YAPS127A

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, YAPS127A (YAP1 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBAM1

Plasmid

#60487

Purpose

This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al BMC Microbiol. 2011 Feb 22;11:38. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE47

Plasmid

#90385

Purpose

IFNB1 - Interferon beta gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

IFNB1

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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piggyFlex

Plasmid

#218234

Purpose

A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.

Depositor

Jay Shendure

Article

Chardon et al Nat Commun. 2024 Sep 18;15(1):8209. doi:

Type

Empty backbone

Use

CRISPR

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

Plasmid

#205447

Purpose

human AAVS1-targeting plasmid for expression of mouse CR1

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCR1

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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