159,371 results

1. pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40

   Plasmid

   #100030

   Purpose

   AAV expression of ArcLightD

   Depositor

   Lawrence Cohen

   Article

   Storace et al Sci Rep. 2015 May 13;5:10212. doi: 10.10

   Insert

   ArcLightD
   

   Use

   AAV

   Tags

   2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CBA

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-FAB(ER-mito)

   Plasmid

   #214423

   Purpose

   Detection of ER-mito contact site

   Depositor

   Chi-Lun Chang

   Article

   Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

   Insert

   RspAN-FRB-V5-Sec61_IRES_CFAST10-20nm-Halo-mito
   

   Use

   Lentiviral

   Tags

   CFAST10-Halo and RspAN-FRB-V5

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBA904

   Plasmid

   #122238

   Purpose

   Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA with cs1 in stem loop 2 of the constant region
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pME-Cas9

   Plasmid

   #63154

   Purpose

   Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Insert

   Cas9
   

   Use

   CRISPR; Tol2 middle entry vector for gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19/CF-mChe

   Plasmid

   #183166

   Purpose

   Construct for transient expression of cytoplasmic foci-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   CF-mCherry
   

   Use

   Tags

   AtTZF1, A. thaliana TANDEM ZINC FINGER PROTEIN 1

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19/Tp-mChe

   Plasmid

   #183165

   Purpose

   Construct for transient expression of tonoplast-targeted mCherry in plants.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Insert

   Tp-mCherry
   

   Use

   Tags

   28 aa of A. thaliana CALCINEURIN B-LIKE PROTEIN 2…

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQC V5 MCS IRES Puro

   Plasmid

   #110342

   Purpose

   γ-Retroviral transfer vector for cloning and expressing your gene of interest. V5 N-Terminal tag, IRES-driven Puromycin selection.

   Depositor

   Sandra Martha Gomes Dias

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV0279

   Plasmid

   #149688

   Purpose

   Bacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTag

   Depositor

   Dirk Görlich

   Article

   Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

   Insert

   SUMO_Eu1-MBP
   

   Use

   Tags

   3xFLAG-tag and His14-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. 8522-M01-663

   Plasmid

   #225660

   Purpose

   Lentiviral expression of a cancer stem cell reporter that responds to presence of Sox2/Oct4 or their paralogs (green configuration)

   Depositor

   Dominic Esposito

   Article

   Tang et al Stem Cell Reports. 2015 Jan 13;4(1):155-

   Insert

   dsCopGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SORE6-mCMVp

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. LV-hGFAP-GFP

    Plasmid

    #183906

    Purpose

    Expression of GFP under the human GFAP ABC1D promoter

    Depositor

    Chun-Li Zhang

    Article

    Niu et al Nat Cell Biol. 2013 Oct;15(10):1164-75.

    Insert

    GFP (GFAP Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CD9-mEGFP

    Plasmid

    #182864

    Purpose

    Localization: Tetraspanin/Membrane

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    human CD9 (CD9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. R777-E160 Hs.PDPK1-nostop

    Plasmid

    #70444

    Purpose

    Gateway ORF clone of human PDPK1 [NM_002613.4] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PDPK1 (PDPK1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-cT3G-cERP2-RUNX1

    Plasmid

    #192928

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of RUNX1

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    RUNX1 (RUNX1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-POLE1

    Plasmid

    #215140

    Purpose

    Expresses mCherry-POLE1 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    POLE1 (POLE Human)
    

    Use

    Retroviral

    Tags

    mCherry_FWR

    Expression

    Mammalian

    Mutation

    Silent mutations in amino acid 80-86 and 211-217 …

    Promoter

    CMV

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEMS1932

    Plasmid

    #79781

    Purpose

    High copy number pUC vector containing Ple259 (PAX6 MiniPromoter) insert

    Depositor

    Elizabeth Simpson

    Article

    Hickmott et al Mol Ther Methods Clin Dev. 2016 Aug 10;3

    Insert

    Ple259
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    Expression

    Mutation

    Promoter

    Ple259 (PAX6 MiniPromoter)

    Available since

    Sept. 14, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pEGFP-MRLC T18A,S19A

    Plasmid

    #35681

    Depositor

    Tom Egelhoff

    Article

    Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

    Insert

    MRLC1 (MYL9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    T18A and S19A

    Promoter

    CMV

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

17. TRBV19

    Plasmid

    #123453

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV19 (TRBV19 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-hSyn-ADAR2-2a-Fezf2 sesRNA-2a-tTA2-WPRE

    Plasmid

    #239027

    Purpose

    Expression of ADAR2, sesRNA in mammalian cells with hSyn promoter, with tTA2 as efRNA and ADAR2 overexpression.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for mouse Fezf2 (Fezf2 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF1777

    Plasmid

    #144310

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    WHSC1 (NSD2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lifeact-3xmNG::LEU2

    Plasmid

    #227594

    Purpose

    Integration vector for expressing Lifeact-3xmNeonGreen

    Depositor

    Bruce Goode

    Article

    Wirshing et al Mol Biol Cell. 2024 Sep 1;35(9):mr7. doi

    Insert

    Lifeact-3xmNeonGreen::LEU2
    

    Use

    Tags

    3xmNeonGreen

    Expression

    Yeast

    Mutation

    Truncated ABP140 (aa 1-17 only)

    Promoter

    Abp140

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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21. pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

    Plasmid

    #223115

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

    Plasmid

    #223116

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. lenti-gRNA

    Plasmid

    #226867

    Purpose

    Lentiviral expression of Sp-gRNA with BlpI and BstXI restriction sites for gRNA spacer cloning. Also expresses BFP.

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    Lenti Sp-gRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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24. mScarletI-T2A-mTq2 pcDNA3.1

    Plasmid

    #230048

    Purpose

    Expresses mScarletI-T2A-mTq2 in human cells

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2024.11.29.626064

    Insert

    mScarletI-T2A-mTq2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. MSCV-FoxP3-IRES-Thy1.1

    Plasmid

    #229616

    Purpose

    Mammalian expression of full length mouse FoxP3

    Depositor

    Sun Hur

    Insert

    full length FoxP3 (Foxp3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MSCV-FoxP3-R337Q-IRES-Thy1.1

    Plasmid

    #229617

    Purpose

    Mammalian expression of full length mouse FoxP3 (R337Q)

    Depositor

    Sun Hur

    Insert

    full length FoxP3, carrying single point mutation R337Q (Foxp3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R337Q

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MSCV-FoxP3-A372S-IRES-Thy1.1

    Plasmid

    #229618

    Purpose

    Mammalian expression of full length mouse FoxP3 (A372S)

    Depositor

    Sun Hur

    Insert

    full length FoxP3, carrying single point mutation A372S (Foxp3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A372S

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MSCV-FoxP3-R337Q/A372S-IRES-Thy1.1

    Plasmid

    #229619

    Purpose

    Mammalian expression of full length mouse FoxP3 (R337Q, A372S)

    Depositor

    Sun Hur

    Insert

    full length FoxP3, carrying double mutation R337Q/A372S (Foxp3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R337Q/A372S

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PCDH-CMV-MCS-EF1a-gfp-T2A-puro_HSALNT0217290

    Plasmid

    #197989

    Purpose

    For the overexpression experiments, full length of the transcript (HSALNT0217290) for HSALNG0104472 was inserted.

    Depositor

    Bo Wang

    Article

    Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

    Insert

    HSALNT0217290
    

    Use

    Lentiviral

    Tags

    lac

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. siRNA-resistant pLV-SigmaR1-mNeonGreen

    Plasmid

    #226569

    Purpose

    Encodes siRNA resistant SigmaR1 protein labeled with mNeonGreen (lentiviral vector)

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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