We narrowed to 9,389 results for: Coli
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Plasmid#71326PurposeNADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterinsulated waaHp from E. coliAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVR2
Plasmid#84719PurposeE. coli RecA promoter followed by stretch with no A's followed by riboswitch for single-round transcriptionDepositorInsertsE. coli RecA promoter
stretch lacking A's followed by 5' UTR of queC gene
UseTagsExpressionBacterialMutation12 nt stretch lacking A's inserted before 5&…PromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ala-eK-Flv
Plasmid#83393Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Ile-eK-Flv
Plasmid#83392Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
His8:MBP-tev-Tyr-eK-Flv
Plasmid#83391Purposebacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studiesDepositorInsertflavin binding protein MioC
UseSynthetic BiologyTags3xHA, 8xHis, and MBPExpressionBacterialMutationPromoterAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKP968
Plasmid#167653PurposeExpress colicinB-His6, D17C, M261CDepositorInsertcba-his6 D17C, M261C
UseTagsExpressionBacterialMutationcba-his6 with D17C, M261C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
L5-LexAp65-L2
Plasmid#41437DepositorInsertLexAp65
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJOE6087.3
Plasmid#135082PurposepBR322-based L-rhamnose-inducible vector.DepositorInsertP-rhaP-BAD-eGFP-His tag
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
R4-LexAp65-R3
Plasmid#41438DepositorInsertLexAp65
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGLU_103
Plasmid#225785PurposeThis is the promoter region taken from upstream of Tn5 transposase in pBAM1. It can be considered the "default" promoter to use.DepositorInsertPtn5_[Tn5]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGLU_85
Plasmid#225800PurposeThis is the promoter region taken from upstream of Himar transposase in pMarC9-Kan. It can be considered the "default" promoter to use.DepositorInsertPmarC9_[Himar]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKP996
Plasmid#167654PurposeExpress colicinB-His6, T18C, M261CDepositorInsertcba-his6 T18C, M261C
UseTagsExpressionBacterialMutationcba-his6 with T18C, M261C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP995
Plasmid#167655PurposeExpress colicinB-His6, M19C, M261CDepositorInsertcba-his6 M19C, M261C
UseTagsExpressionBacterialMutationcba-his6 with M19C, M261C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP997
Plasmid#167656PurposeExpress colicinB-His6, V20C, M261CDepositorInsertcba-his6 V20C, M261C
UseTagsExpressionBacterialMutationcba-his6 with V20C, M261C mutationsPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2264
Plasmid#167660PurposeExpress colicinB-His6, A121C, Y254CDepositorInsertcba-his6, A121C, Y254C
UseTagsExpressionBacterialMutationcba-his6, I121C, Y254CPromoterAvailable sinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBT1-proD-mCherry
Plasmid#65823PurposeConst. mCherry expression , proD promoter, E. coli ExpressionDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterproD E . coli Constitutive PromoterAvailable sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZH_tdPP7cp_mCherry
Plasmid#118267PurposeLow-noise, tetracycline inducible expression of tdPP7cp-mCherryDepositorInserttdPP7cp-mCherry
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-TALEN-Rep
Plasmid#45964DepositorInsertbeta-Galactosidase
UseTagsExpressionMammalianMutationduplicated aa 1-135PromoterCMVAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZH_mPP7cp_mCerulean3
Plasmid#118262PurposeLow-noise, tetracycline inducible expression of mPP7cp-mCerulean3DepositorInsertmPP7cp-mCerulean3
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH_tdPP7cp_mCerulean3
Plasmid#118263PurposeLow-noise, tetracycline inducible expression of tdPP7cp-mCerulean3DepositorInserttdPP7cp-mCerulean3
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH_mPP7cp_mCherry
Plasmid#118266PurposeLow-noise, tetracycline inducible expression of mPP7cp-mCherryDepositorInsertmPP7cp-mCherry
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH_tdPP7cp_ECFP
Plasmid#118265PurposeLow-noise, tetracycline inducible expression of tdPP7cp-ECFPDepositorInserttdPP7cp-ECFP
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZH_mPP7cp_ECFP
Plasmid#118264PurposeLow-noise, tetracycline inducible expression of mPP7cp-ECFPDepositorInsertmPP7cp-ECFP
UseTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Rab-Rep
Plasmid#45967DepositorInsertbeta-Galactosidase
UseTagsExpressionMammalianMutationduplicated aa 1-135PromoterCMVAvailable sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA C12 ecDHFR DD YFP HA
Plasmid#192815PurposeExpresses an N-terminal enhanced destabilizing domain (DD) version of E. coli DHFR with higher basal turnover in mammalian systems. Contains missense mutations W74R/T113S/E120D/Q146L.DepositorInsertE. coli dihydrofolate reductase
UseTagsenhanced yellow fluorescent protein and hemagglut…ExpressionMammalianMutationW74R/T113S/E120D/Q146LPromoterCMVAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-recA56
Plasmid#89962PurposeModified from pEM-Cas9HF1 (Addgene ID: 89961) to include constitutive recA56 to block recA-mediated double-strand break repair.DepositorInsertsCas9HF1
recA56
UseTagsExpressionBacterialMutationN497A/R661A/Q695A/Q926APromoterpTet and proDAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6410
Plasmid#47049PurposeBglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-MCC
Plasmid#133307Purpose"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisationDepositorInsertmicrocin-V bacteriocin cassette (cvaC E. coli)
UseSynthetic BiologyTagsExpressionBacterialMutationN112D (please see depositors comments)PromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPReporter-mCherry
Plasmid#65008PurposeE. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterUser-definedAvailable sinceMay 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSF-Ivy-his-hLYZ
Plasmid#83920PurposeCo-expresses a hisx6 tagged copy of the Ivy protein and the wild type human lysozyme protein, both in the cytoplasmic compartment.DepositorInsertsUseTagsIvy protein has C-terminal hisx6 tagExpressionBacterialMutationIvy leader sequence deleted and replaced.PromoterT7 and t7Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-RgTALsyn-HpaBC
Plasmid#100949PurposeExpression of RgTALsyn, HpaB, and HpaC from inducible T7 promoterDepositorInsertsRgTALsyn
HpaB
HpaC
UseSynthetic BiologyTagsExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterT7Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSMART-ala10
Plasmid#87135PurposeNADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli ExpressionDepositorUseTagsExpressionBacterialMutationD196A, L197RPromoterwaaHp from E. coli as part of operon with ald* an…Available sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PatoG-OG241
Plasmid#72839PurposeInduced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the native atoDAEB promoter (Pato) from E. coli DH5A. OG241 backbone.DepositorInsertPatoG
UseTagsExpressionBacterialMutationPromoterPatoGAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK597
Plasmid#71121PurposeProduces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coliDepositorInsertformyl-CoA:oxalate CoA-transferase
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRAR064
Plasmid#185031Purpose400 nt l31 promoter region, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationNucleotides of rpmE 5'UTR scrambled, Only fi…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR074-control-sfGFP
Plasmid#185032PurposeJ23108 promoter, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationNucleotides of rpmE 5'UTR scrambled, Only fi…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR102
Plasmid#185033Purpose400 nt l31 promoter region, l31 5'UTR U54C mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationU54C mutation of rpmE 5'UTR, Only first 90 n…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR106
Plasmid#185036Purpose400 nt l31 promoter region, l31 5'UTR G79U mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationG79U mutation of rpmE 5'UTR, Only first 90 n…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRAR116
Plasmid#185040Purpose400 nt l31 promoter region, l31 5'UTR U58C and U70C, 90 nt of l31 coding region, glycine linker, sfGFP, trrnBDepositorInsertL31-sfGFP
UseTagsExpressionBacterialMutationU58C and U70C of rpmE of 5'UTR, Only first 9…PromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only