We narrowed to 2,903 results for: BFP

1. pHBS1502 [IBB-GFP-mCherry3E]-[BFP-TDP43 deltaCTD]

   Plasmid

   #133326

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Article

   Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

   Insert

   TARDBP mutant (TARDBP Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of NTD in full-length TDP43

   Available Since

   Nov. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

   Plasmid

   #159798

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2709-pHAGE2-Ef1aL-YAP5SA-UBC-tagBFP-loxP

   Plasmid

   #216478

   Purpose

   lentiviral dual expression of YAP5SA and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   YAP5SA
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Mutation

   S61A, S109A, S127A, S164A, S381A

   Promoter

   EF1aL and UBC

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP

   Plasmid

   #216479

   Purpose

   lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   wtYAP (YAP1) (YAP1 Human)
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Promoter

   EF1aL and UBC

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

   Plasmid

   #233445

   Purpose

   PiggyBac donor plasmid to overexpress a full length ADAR2

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   ADAR2-T2A-PuroR-P2A-BFP
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   EF1a

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin

   Plasmid

   #187956

   Purpose

   FKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   FKBP12_F36V-hHDAC4-SpdCas9-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. p2708-pHAGE2-Ef1aL-dsRed-UBC-tagBFP-loxP

   Plasmid

   #216477

   Purpose

   lentiviral dual expression of dsRed and tagBFP with loxP site for Cre excision

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Inserts

   dsRed
   tagBFP
   

   Use

   Cre/Lox and Lentiviral

   Promoter

   EF1aL and UBC

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

   Plasmid

   #158651

   Purpose

   making transgenics expressing a UAS driven YFP and NTR 2.0

   Depositor

   Jeff Mumm

   Article

   Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

   Insert

   5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:BFP
   

   Use

   Tol2-based transgenic expression in zebrafish/oth…

   Mutation

   F70A/F108Y

   Available Since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLPB2B-FKBP12_F36V-KRAB-SpdCas9-tagBFP-PGK-Blasticidin

   Plasmid

   #187957

   Purpose

   FKBP12 (F36V mutant) degron-tagged KRAB-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   FKBP12_F36V-KRAB-SpdCas9-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

    Plasmid

    #228252

    Purpose

    pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    SpCas9 P23H sgRNA, mTagBFP2
    

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and U6

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLPB2B-FKBP12_F36V-SpdCas9-KRAB-tagBFP-PGK-Blasticidin

    Plasmid

    #187953

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9-KRAB domain fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-KRAB-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GGS

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDV21_feedback_entry_TagBFP-2A-MCS-2A-NLuc-2A-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200403

    Purpose

    Entry vector for closed-loop ADAR-based RNA sensors (DART VADAR). Expresses TagBFP constitutively. Expresses NanoLuc and MCP-ADAR upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Closed-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP

    Plasmid

    #164038

    Purpose

    Expression of dCas9-14xGFP11 in mammalian cells

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    dCas9-14xGFP11
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    dSV40

    Available Since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. p2711-pHAGE2-Ef1aL-TAZ4SA-UBC-tagBFP-loxP

    Plasmid

    #216480

    Purpose

    lentiviral dual expression of TAZ4SA and tagBFP with loxP site for Cre excision

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    TAZ4SA (WWTR1 Human)
    tagBFP
    

    Use

    Cre/Lox and Lentiviral

    Mutation

    S66A, S89A, S117A, S311A

    Promoter

    EF1aL and UBC

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-human U6-sgRNA-EF1Alpha-puro-T2A-BFP

    Plasmid

    #118594

    Purpose

    Parental vector for the CRISPRa libraries. Expresses an sgRNA from the human U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    sgRNA/Puro-T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSynI-fDIO(TagBFP)-KoNLS-Cre(MtoL)

    Plasmid

    #180587

    Purpose

    FdCd

    Depositor

    Won Do Heo

    Article

    Jung et al Nat Commun. 2019 Jan 18;10(1):314. doi:

    Insert

    Cre
    

    Use

    AAV

    Available Since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. iE61 PB-Zim3-XTEN-dCas9-mScarlet-puro-BFP

    Plasmid

    #204722

    Purpose

    Constitutive CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKV2-hU6-BbsI-PGK-Puro-TagBFP-WPRE

    Plasmid

    #137994

    Purpose

    Backbone for generation of ClonMapper gRNA barcode library for use in 10X feature barcoding

    Depositor

    Amy Brock

    Article

    Gardner et al Methods Mol Biol. 2022;2394:109-131. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. EK0307 CMV-TO-BFPmut-stop-3UTR(Bdnf) (FLP-IN)

    Plasmid

    #191145

    Purpose

    Expression of a synthetic construct with 3' UTR of murine Bdnf in the 3' UTR of an inactive BFP in mammalian cells.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    3UTR(Bdnf)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV-TO

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLPB2B-FKBP12_F36V-SpdCas9-hHDAC4-tagBFP-PGK-Blasticidin

    Plasmid

    #187954

    Purpose

    FKBP12 (F36V mutant) degron-tagged dCas9-hHDAC4 fused to tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    FKBP12_F36V-SpdCas9-hHDAC4-tagBFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTRIP-CMV-mtagBFP2-2A-FLAG-cGAS E225A/D227A

    Plasmid

    #102604

    Purpose

    Lentivector to express Flag-tagged and catalytically-inactive cGAS and mTagBFP

    Depositor

    Nicolas Manel

    Article

    Gentili et al Science. 2015 Sep 11;349(6253):1232-6. d

    Insert

    cGAS (CGAS Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    E225A-D227A

    Promoter

    CMV

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHR-GAL4-IL35-IRES-mCherry-PGK-BFP

    Plasmid

    #223210

    Purpose

    Lentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    IL35 (IL12A Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHR-GAL4-PDL1-IRES-mCherry-PGK-BFP

    Plasmid

    #223208

    Purpose

    Lentiviral vector - mCherry reporter and PDL1 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

    Depositor

    Wendell Lim

    Article

    Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

    Insert

    PDL1 (CD274 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pU6-mir144~451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164793

    Purpose

    Express miR-144~451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    miR-144~451 (MIR451A, MIR144 Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha and U6

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid

    #187945

    Purpose

    dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

    Depositor

    Edda Schulz

    Article

    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

    Insert

    SpdCas9-tagRFPt-P2A-tagBFP
    

    Use

    Synthetic Biology; Piggybac

    Tags

    NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt

    Expression

    Mammalian

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. JDW 829 (pTol2-kdrl:V5-mTagBFP2-hsKRAS4A-G12D-ac/Y)

    Plasmid

    #156415

    Purpose

    kdrl promoter driving V5-tagged mTagBFP2 fused to human KRAS4A-G12D with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites

    Depositor

    Joshua Wythe

    Article

    Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

    Insert

    human mutant KRAS4A-G12D (KRAS Human)
    

    Use

    Zebrafish transgenesis

    Tags

    mTagBFP2

    Mutation

    mutant KRAS4A-G12D

    Promoter

    kdrl

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHBS1505 [IBB-GFP-mCherry3E]-[BFP-TDP43 FYW-S]

    Plasmid

    #133329

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    All FYW to S in CTD of full-length TDP43

    Available Since

    Nov. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAAV-Syn-SomArchon-mTagBFP2-p2A-CoChR-Kv2.1

    Plasmid

    #206006

    Purpose

    Expression of SomArchon-mTagBFP2 and CoChR under Syn promoter

    Depositor

    Xue Han

    Article

    Lowet et al Cell Rep. 2023 Aug 29;42(8):112906. doi:

    Insert

    SomArchon-mTagBFP2-P2A-CoChR-Kv2.1
    

    Use

    AAV

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MADR pDonor TagBFP2-V5-HRAS G12V WPRE RCE

    Plasmid

    #131730

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion compatible with RCE:loxP mouse strain

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    TagBFP2-V5 tag

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFP

    Plasmid

    #192609

    Purpose

    Lentiviral vector for constitutive expression of LexA-CIB1-biLINuS-P2A-tagBFP.

    Depositor

    Yingxiao Wang

    Article

    Huang et al Sci Adv. 2020 Feb 19;6(8):eaay9209. doi:

    Insert

    LexA-CIB1-biLINuS-P2A-tagBFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    tagBFP

    Promoter

    PGK

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHIE067 CAG mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138747

    Purpose

    Integration Vector, assembled with pHIE041 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    CAG
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHBS1396 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT]

    Plasmid

    #118804

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pEF1α-squirrel-monkey-retroCHMP3-T2A-H2B-BFP

    Plasmid

    #154185

    Purpose

    expresses squirrel monkey retroCHMP3 with self-cleaving tag in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Tags

    HA and T2A-H2B-BFP

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1-TagBFP-P2A-Pre-SPHERE-sfVenus-iGluSnFR

    Plasmid

    #187097

    Purpose

    Glutamate sensor for localization to membrane contatct

    Depositor

    Kotaro Oka

    Article

    Shindo et al bioRxiv 2022

    Insert

    Pre-SPHERE-sfVenus-iGluSnFR
    

    Tags

    TagBFP-P2A

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAGGS-mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2

    Plasmid

    #128167

    Purpose

    Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, GSAx9 linker)

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHIE058 ZF43x0 mKate2 EF1a EBFP2-P2A-BlastR

    Plasmid

    #138739

    Purpose

    Integration Vector, assembled with pHIE042 (TUPV1), pJM451 (TUPV2), pPD612

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x0
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Dmap1(SDM)

    Plasmid

    #117139

    Purpose

    Expression vector encoding BFP-BSD-HA-Dmap1 resistant against gRNA encoded by pKLV2-U6(gDmap1 v4)--PGK-Puro-BFP

    Depositor

    Kosuke Yusa

    Article

    Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

    Insert

    HA-Dmap1 (mutagenised) (Dmap1 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    modified BFP, modified Dmap1 CDS

    Promoter

    human U6 promoter, human EF1a promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHBS1330 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 WT]

    Plasmid

    #107839

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Insert

    TARDBP (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    Wildtype in splicing reporter

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. VEE-mScarlet3-IRES-E3-NSs-L*-EGFP-IRES-moxBFP

    Plasmid

    #242410

    Purpose

    Self-amplifying RNA (saRNA) construct expresses dsRNA pathway inhibitors (vaccinia virus E3, Toscana virus NSs, Theiler’s virus L*) and moxBFP (in place of inflammatory signaling inhibitors).

    Depositor

    Ewan St. John Smith

    Article

    Lim et al bioRxiv 2024.09.24.614636

    Inserts

    mScarlet3
    IRES-E3-NSs-L*-EGFP
    IRES-moxBFP
    

    Use

    Mouse Targeting; Self-amplifying rna

    Expression

    Mammalian

    Available Since

    Sept. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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40. pEJS2782 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP

    Plasmid

    #240518

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion with restriction enzyme sites in the 3' UTR for use as microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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41. Gai-mTagBFP-3BEY-P2A-Gid-4LTB-GFP

    Plasmid

    #240137

    Purpose

    Mammalian plasmid expression both components of synDrops separated using P2A

    Depositor

    Liam Holt

    Article

    Shu et al PRX Life. 2024 Sep;2(3):033001. doi: 10.

    Inserts

    Gai-mTagBFP-3BEY
    Gid-4LTB-GFP
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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42. pRhobin2-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS

    Plasmid

    #239020

    Purpose

    Co-overexpression of Rhobin2-H2B-mNeonGreen3K fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

    Depositor

    Bo Huang, William DeGrado

    Article

    Chen et al bioRxiv 2025.06.24.661379

    Insert

    Rhobin2-H2B (H2BC21 Human, Synthetic)
    

    Tags

    TagBFP and mNeonGreen3K

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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43. pRhobin9-H2B-mNG3K-IRES-NLS-mTagBFP-2xNLS

    Plasmid

    #239021

    Purpose

    Co-overexpression of Rhobin9-H2B-mNeonGreen3K fusion protein and NLS-mTagBFP-2xNLS as a nuclear labeling marker

    Depositor

    Bo Huang, William DeGrado

    Article

    Chen et al bioRxiv 2025.06.24.661379

    Insert

    Rhobin9-H2B (H2BC21 Human, Synthetic)
    

    Tags

    TagBFP and mNeonGreen3K

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    June 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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44. pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)

    Plasmid

    #238185

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF4 (KLF4 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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45. pEJS1355 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP

    Plasmid

    #226114

    Purpose

    Mammalian expression of AcrIIC3-mCherry fusion without microRNA target sites in the 3' UTR as a control reporter of microRNA repression. BFP expressed separately as a transfection control.

    Depositor

    Erik Sontheimer

    Article

    Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

    Insert

    AcrIIC3
    

    Tags

    FLAG and mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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46. LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

    Plasmid

    #233442

    Purpose

    PiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP
    

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9

    Plasmid

    #231907

    Purpose

    For packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 virus-like particles (VLPs)

    Depositor

    Sriram Neelamegham

    Article

    Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    EGFP-P2A-dTomato-T2A-TagBFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)

    Plasmid

    #229819

    Purpose

    A gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

    Plasmid

    #229831

    Purpose

    A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAS3-npr-9P::AI::lgc-47::SL2::tagBFP::unc-54-3'UTR

    Plasmid

    #225923

    Purpose

    Expressing LGC-47 in AIB neuron

    Depositor

    Andrew Leifer

    Article

    Kumar et al bioRxiv [Preprint]. 2024 Mar 27:2024.03.

    Insert

    LGC-47 (lgc-47 Nematode)
    

    Expression

    Worm

    Promoter

    npr-9

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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