We narrowed to 7,454 results for: Lif;
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Plasmid#80453PurposeSpCas9 mammalian expression vectorDepositorInsertSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailable sinceAug. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-SAC1-FKBP
Plasmid#108129PurposeRecruitable SAC1DepositorInsertmCherry:SACM1L:FKBP1A(3-108) (SACM1L Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBpuro-COX2-EBFP2
Plasmid#184720PurposeExogenous COX2DepositorInsertCOX2-EBFP2 (PTGS2 Human, Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
FKBP-AP180-GFP
Plasmid#186577PurposeExpression of the fusion protein FKBP-AP180-GFP. Uses rat AP180 residues 328-896.DepositorInsertAP180 (Snap91 Rat)
UseTagsFKBP N-terminal GFP C-terminalExpressionMammalianMutationPromoterAvailable sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMA-SpCas9-g7
Plasmid#80790PurposeBsaI-based cloning of SpCas9 gRNA guide sequence, position 7/17/27 in the arrayDepositorInsertCRISPR gRNA expression cassette (for SpCas9)
UseCRISPRTagsnaExpressionMammalianMutationPromoterU6Available sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
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tCRMod-GGSGGS9
Plasmid#111762PurposeExpresses soluble Clover-mRuby2 tension sensor module with 9-repeat GGSGGS extensible domainDepositorInsertClover-GGSGGS9-mRuby2 fusion
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-mTagBFP-T2A-TetOn3G
Plasmid#179888PurposeExpresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)DepositorInsertTetOn3G
UseLentiviralTagsmTagBFPExpressionBacterial and MammalianMutationPromoterEF1aAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TetO-U2B''-fSNAPc
Plasmid#106988Purposeopen reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sitesDepositorInsertUB2" (SNRPB2 Human)
UseTagsfSNAPExpressionMutationPromoterAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4
Plasmid#87733PurposeBacterial expression of full-length S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
acta1-mRuby2-dGBP
Plasmid#163855PurposeZebrafish muscle specific expression of mRuby2-dVHH[GFP]DepositorInsertdVHH[GFP]
UseIn vitro transcription of rnaTagsRuby2ExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-Z3EV
Plasmid#157659PurposeGFP gene and gRNA under Z3EV expression control (estradiol sensor), with Z3EV transcription factor to insert in the genome.DepositorInsertGFP-gRNA NatMX Z3EV
UseInsert storage (replicative in e. coli)TagsExpressionMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+)_NADP-Snifit
Plasmid#113913PurposePlasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+DepositorInsertNADP-Snifit
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-proCaspase-3-TEVp
Plasmid#166607PurposeEncodes Cre-dependent procaspase 3 and TEVp under control of the TREDepositorInsertprocaspase3- TEVp
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceJune 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-SAC1C389S
Plasmid#108128PurposeRecruitable inactive SAC1DepositorInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S (SACM1L Human)
UseTagsExpressionMammalianMutationSACM1L(C389S)PromoterCMVAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-TVA-oG-mCherry
Plasmid#166602PurposeEncodes Cre-dependent TVA-oG-mCherry under control of the TREDepositorInsertTVA-oG-mCherry
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
raACR-pmCerulean3-N1
Plasmid#204959PurposeExpression of the MsACR1 mutant raACR as a fusion protein with mCerulean3 in mammalian cellsDepositorInsertraACR
UseTagsmCerulean3ExpressionMammalianMutationPromoterCMV (+enhancer)Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJFRC7-UAS-NPRR_ANP
Plasmid#133243PurposeFluorescent reporter for neuropeptide release imagingDepositorInsertrat atrial natriuretic peptide-fused codon-optimized GCaMP6s
UseTagsExpressionInsectMutationPromoterhsp70Available sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMA-SpCas9-g10
Plasmid#80793PurposeBsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the arrayDepositorInsertCRISPR gRNA expression cassette (for SpCas9)
UseCRISPRTagsnaExpressionMammalianMutationPromoterU6Available sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMA-SpCas9-g9
Plasmid#80792PurposeBsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the arrayDepositorInsertCRISPR gRNA expression cassette (for SpCas9)
UseCRISPRTagsnaExpressionMammalianMutationPromoterU6Available sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMA-SpCas9-g8
Plasmid#80791PurposeBsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the arrayDepositorInsertCRISPR gRNA expression cassette (for SpCas9)
UseCRISPRTagsnaExpressionMammalianMutationPromoterU6Available sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-eGFP-L10a
Plasmid#166605PurposeEncodes Cre-dependent eGFP-L10a under control of the TREDepositorInserteGFP-L10a
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn spCas9
Plasmid#179725Purposehuman synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITIDepositorInsertspCas9
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-GFP-2A-SynmRuby
Plasmid#166608PurposeEncodes Cre-dependent GFP-2A-synaptophysinmRuby under control of the TREDepositorInsertGFP-2A-syn
UseAAVTagsExpressionMutationPromotertetracycline response elementAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pF(UG) hSyn SYT7alpha
Plasmid#179713PurposeLentiviral expression of mouse Syt7DepositorInsertSYT7 (Syt7 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mCherry-GS linker-Zdk1
Plasmid#125226PurposeExpresses mCherry-GS linker-Zdk1 in mammalian cellsDepositorInsertmCherry-GS linker-Zdk1
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PB-PGK-tetR-KRAB-2A-Puro
Plasmid#129536PurposePiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.DepositorInsertPB-PGK-tetR-KRAB-2A-Puro
UseUnspecifiedTagsExpressionMutationPromoterhPGK promoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TetO-U1-70K-fSNAPc
Plasmid#106987Purposeopen reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sitesDepositorInsertU1-70K (SNRNP70 Human)
UseTagsfSNAPExpressionMutationPromoterAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
tCRMod-GGSGGS7
Plasmid#111761PurposeExpresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domainDepositorInsertClover-GGSGGS7-mRuby2 fusion
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
tCRMod-GGSGGS5
Plasmid#111760PurposeExpresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domainDepositorInsertClover-GGSGGS5-mRuby2 fusion
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-backbone
Plasmid#172517PurposeYeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertionDepositorInsertgRNA cassette
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMito-dCherry-FRB
Plasmid#186573PurposeExpression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cellsDepositorInsertDark MitoTrap
UseTagsExpressionMammalianMutationMutation of "K70N" to render mCherry no…PromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-TK-Firefly-cjBCCIP
Plasmid#122278PurposeLentiviral vector for stable expression of Firefly luciferase.DepositorInsertsFirefly luciferase
cjBCCIP 3'UTR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceApril 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1553 - pAAV EF1a EGFP-KASH
Plasmid#131682PurposeAn adeno-associated viral vector expressing nuclear envelope-embedded eGFPDepositorInsertEGFP-KASH
UseAAVTagsKASHExpressionMutationPromoterAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV syn CCR6-eLOV-TEVcs-tTA
Plasmid#173116PurposeExpresses CCR6 receiver construct in neuronsDepositorInsertCCR6-eLOV-TEVcs-tTA
UseAAVTagsExpressionMutationPromoterSynapsinAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRecLV103-GFP-PGBD5
Plasmid#65409PurposeLentiviral expression vector for fusion of GFP with human PGBD5DepositorInsertPGBD5 (PGBD5 Human)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRv2puro-Tyr
Plasmid#170293PurposeA knock-out vector for the mouse tyrosinase.DepositorInsertA gRNA targeting the mouse tyrosinase gene.
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nephrin
Plasmid#186134PurposeFor expression of recombinant nephrin in bacteria cellsDepositorInsertNPHS1 (NPHS1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationAmino acid residues 1174-1223, with mutations: Y1…PromoterLacAvailable sinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUE1-TSP-ORF68
Plasmid#162650PurposeExpresses ORF68 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage siteDepositorInsertORF68
UseTagsN-terminal 2xStrep-Tag II and HRV 3C siteExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pJAG98-mTurquoise2-Nuf2
Plasmid#80760PurposeExpress Nuf2 N-terminally labeled with mTurquoise2DepositorInsertNuf2 (NUF2 Human)
UseRetroviralTagsmTurquoise2ExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125231PurposeFor generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirusDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
CALNL-RARa-VP16
Plasmid#185560PurposeCre Dependent ubiquitous expression of constitutively active Retinoic Acid Receptor alphaDepositorInsertRARa-VP16 (Rara Mouse)
UseTagsVP16ExpressionMammalianMutationPromoterCAGAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2
Plasmid#87644PurposeFor creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2DepositorInsertmyr::SNAPf>myr::CLIPf>myr::Halo2
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
P303-FBP_2
Plasmid#139693PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, without recycling gene CPHDepositorInsertp35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:AtuOCS, pCmYLCV:NnHisps:tHsp, p35S:NnLuz:tAtuOcs
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-Alk8
Plasmid#207615PurposeZebrafish Alk8 BMP receptor kinase domain fused to VfLOVDepositorInsertAlk8 (acvr1l) kinase domain + VfLOV domain (acvr1l Zebrafish, Vaucheria frigida)
UseTagsExpressionMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1(+)-PM-CaNARi
Plasmid#64850PurposePlasma membrane-targeted FRET biosensor for monitoring calcineurin (CaN) activation.DepositorInsertLyn-CaNARi
UseTagsCerulean, N-terminal targeting sequence from Lyn …ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only