-
Plasmid#61344PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS9/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS9 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-EGFP-ChIEF
Plasmid#172371PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertEGFP + ChIEF
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN43
Plasmid#61346PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS11/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS11 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-RO1.7-dnHIF
Plasmid#206343PurposeAAV plasmid expressing dominant negative HIF1A in photoreceptorsDepositorInsertdominant negative hif1a
UseAAVTagsExpressionMutationdominant negative HIF1APromoter1.7kb human red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2-Opto-Alk3
Plasmid#207614PurposeZebrafish Alk3 BMP receptor kinase domain fused to VfLOVDepositorInsertAlk3 (bmpr1aa) kinase domain + VfLOV domain (bmpr1aa Zebrafish, Vaucheria frigida)
UseTagsExpressionMutationPromoterAvailable sinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SARS-CoV-2 S1 RBD
Plasmid#167013PurposeGateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2DepositorInsertS (S SARS-CoV-2 isolate Wuhan-Hu-1)
UseTagsExpressionMammalianMutationNo stop codonPromoterAvailable sinceApril 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
gRNA-NONO-1
Plasmid#127654PurposeKnock-out of human NONO (guide only)DepositorInsertNONO sgRNA (NONO Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE
Plasmid#236241PurposeLentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmeraldDepositorInsertCaV1.2 (CACNA1C Rabbit)
UseLentiviralTagsmEmeraldExpressionMutationPromoterhuman Synapsin 1Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE
Plasmid#236239PurposeLentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmeraldDepositorInsertCaV2.1 (CACNA1A Rabbit)
UseLentiviralTagsmEmeraldExpressionMutationPromoterhuman Synapsin 1Available sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE
Plasmid#236240PurposeLentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmeraldDepositorInsertCaV2.2 (CACNA1B Rabbit)
UseLentiviralTagsmEmeraldExpressionMutationPromoterhuman Synapsin 1Available sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-vCASphi_E9t_MCS_version2
Plasmid#197961PurposeT-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter.DepositorInsertpco-vCasphi-2
UseCRISPRTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-nCASphi_E9t_MCS_version2
Plasmid#197962PurposeT-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter.DepositorInsertpco-nCasphi-2
UseCRISPRTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pcoCASphi_E9t_MCS_version2
Plasmid#197950PurposeT-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal.DepositorInsertpcoCasphi-2
UseCRISPRTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABCB10-ChR2-eYFP
Plasmid#206037PurposeExpress ChR2 in mitochondriaDepositorInsertChR2
UseTagsABCB10 mitochondrial leading sequence and EYFPExpressionMammalianMutationPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-PSD95-mCherry
Plasmid#125694PurposeExogenous expression of PSD95 (red fluorescence)DepositorInsertPSD95-mCherry
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-PhoCl-mCherry
Plasmid#87690PurposeExpress NES-PhoCl-mCherry in mammalian cellsDepositorInsertNES-PhoCl-mCherry
UseTagsnuclear export signalExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ERT2-PhoCl-Gal4VP16-PhoCl-ERT2
Plasmid#87693PurposeExpress ERT2-PhoCl-Gal4VP16-PhoCl-ERT2 in mammalian cellsDepositorInsertERT2-PhoCl-Gal4VP16-PhoCl-ERT2
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NLS-PhoCl-mCherry
Plasmid#87691PurposeExpress NLS-PhoCl-mCherry in mammalian cellsDepositorInsertNLS-PhoCl-mCherry
UseTagsnuclear localization signalExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-PhoCl-mCherry-myc
Plasmid#87692PurposeExpress PhoCl-mCherry with C-term myc tag in mammalian cellsDepositorInsertPhoCl-mCherry-myc
UseTagsmyc tagExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-YWHAE
Plasmid#31562DepositorInserttyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Syn-PSD95.FingR-eGFP
Plasmid#126212PurposeLabeling of excitatory synapses (green fluorescence) in dividing cellsDepositorInsertPSD95.FingR-eGFP
UseRetroviralTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-WP1-CRY2PHR-VP64
Plasmid#171758PurposeLentiviral vector for constitutive expression of fusion protein WP1-CRY2PHR-VP64DepositorInsertWP1-CRY2PHR-VP64
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pcoCASphi_E9t_MCS_version1
Plasmid#197937PurposeT-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminal.DepositorInsertpcoCasphi-2
UseCRISPRTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-LexA-CIB1-biLINuS
Plasmid#192607PurposeLentiviral vector for constitutive expression of LexA-CIB1-biLINuSDepositorInsertLexA-CIB1-biLINuS
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-eGFP-P2A-CRY2-VPR
Plasmid#192610PurposeLentiviral vector for constitutive expression of eGFP-P2A-CRY2-VPR.DepositorInserteGFP-P2A-CRY2PHR-VPR
UseLentiviral and Synthetic BiologyTagseGFPExpressionMutationPromoterPGKAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOME0136MR
Plasmid#178399PurposeKSHV Kaposinclouster_RevCompl-KapATGdel_SynDepositorInsertKaposinclouster_RevCompl-KapATGdel_Syn
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CRY2-iSH2
Plasmid#66839PurposeExpression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.DepositorInsertiSH2
UseTagsmCherry-CRY2ExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lyn11-CIBN-GFP
Plasmid#79572Purposeexpression of plasma membrane signal from Lyn11 fused to CIBN and tagged with GFPDepositorInsertLyn11
UseTagsCIBN (CRY2-binding domain) and EGFPExpressionMammalianMutationG8R (please see depositor comments below)PromoterCMVAvailable sinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16
Plasmid#163029PurposeFLiCRE TF for neuronal expressionDepositorInsertNrxn3b-MKII-hLOV1-TEVcs-tTA
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-hRIP3 dRHIM
Plasmid#208788PurposeInducibly expresses human RIP3, lacking RHIM domain, fused to light-activatable Cry2DepositorInsertRIP3 (RIPK3 Human)
UseLentiviralTagsCry2 mCherryExpressionMammalianMutationRHIM domain is removedPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 SA-2A-puro-pA_hSYN1_dTomato-SV40pA
Plasmid#196192Purposehuman synapsin promoter driven dTomato expression at human AAVS1 safe harbour locusDepositorInsert2xCHS4_hSYN1_INTRON_dTomato-WPRE-SV40-2xCHS4
UseZnf donorTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16
Plasmid#163031PurposeFLiCRE TF with fast kinetics for neuronal expressionDepositorInsertNrxn3b-MKII-f-hLOV1-TEVcs-tTA
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN34
Plasmid#61343PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS2/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-biNLS2 and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
sg2.02 16x(MS2) MUC4.1
Plasmid#101154PurposegRNA with 16 MCP binding siteDepositorInsertMUC4 (MUC4 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDN80
Plasmid#61349PurposeLINuS-flex: modular LINuS construct enabling introduction of any protein encoding sequence, NES and NLS. The sequence of interest replaces the LexA DBD-MBP-VP64 fragment.DepositorTypeEmpty backboneUseTagsAsLOV2 wt and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB22
Plasmid#61342PurposeMammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)DepositorInsertmCherry
UseTagsGS linker-AsLov2-cMyc1A NLS and PKIt NESExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
sg14x(MS2) MUC4.1
Plasmid#101153PurposegRNA with 14 MCP binding siteDepositorInsertMUC4 (MUC4 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CRY2-CreN
Plasmid#75268PurposeThe second half of the photo-activatable cre recombinaseDepositorInsertsecond half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)
UseAAVTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-ReaChR-citrine
Plasmid#169045PurposeMiddle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insertDepositorInsertReaChR-citrine
UseGateway entry cloning vectorTagscitrineExpressionMutationPromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFP
Plasmid#192609PurposeLentiviral vector for constitutive expression of LexA-CIB1-biLINuS-P2A-tagBFP.DepositorInsertLexA-CIB1-biLINuS-P2A-tagBFP
UseLentiviral and Synthetic BiologyTagstagBFPExpressionMutationPromoterPGKAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
sg2.02 MUC4.1
Plasmid#101152PurposegRNA with two MCP binding sitesDepositorInsertMUC4 (MUC4 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromotermU6Available sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCh-Miro1(∆TM)-SspB
Plasmid#194007PurposeOptogenetic toolDepositorInsertMiro1 (var4, peroxisomal splice variant) (RHOT1 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSYn-DIO-ScramblemiR-IRES-mCitrine
Plasmid#186418PurposeExpression of scrambled shRNA used as a control for knock down experimentsDepositorInsertScramblemiR
UseAAVTagsmCitrineExpressionMutationPromoterhuman Synapsin1Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-Egr3-miR-IRES-mCitrine
Plasmid#186419PurposeExpression of shRNA for the knock down of mouse Egr3DepositorInsertmiREgr3-5D
UseAAVTagsmCitrineExpressionMutationPromoterhuman Synapsin1Available sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAL928
Plasmid#119853Purposeprecusor alkaline phosphatase, phoA, with C169S, C179S, C287S, C337S, A369C and A427CDepositorInsertprecursor alkaline phosphatase, phoA, with mutations C169S, C179S, C287S, C337S, A369C and A427C
UseTagsnoneExpressionBacterialMutationCysteine 169 to Serine, Cysteine 179 to Serine, C…PromoterT7Available sinceDec. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-mMLKL
Plasmid#208784PurposeInducibly expresses mouse MLKL fused to light-activatable Cry2DepositorInsertmCherry mMLKL Cry2 (Mlkl Mouse)
UseLentiviralTagsCry2 and mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-opto-mRipK3
Plasmid#208783PurposeInducibly expresses mouse RipK3 fused to light activatable Cry2DepositorInsertmCherry mRipK3 Cry2 (Ripk3 Mouse)
UseLentiviralTagsCry2 and mCherryExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
EC71171
Plasmid#154069PurposeLevel 0 Golden Gate vector, CRE with U5 intron at 254bp, in pICH41308 backboneDepositorInsertCRE_U5intron
UseSynthetic BiologyTagsExpressionMutationAll BsaI, Esp3I and BPiI restriction sites were r…PromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-RedO-Txnip(C247S)
Plasmid#206341PurposeAAV plasmid expressing mutant TXNIP in photoreceptorsDepositorInsertTxnip (Txnip Mouse)
UseAAVTagsExpressionMutationC247SPromoterhuman red opsinAvailable sinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only