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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,031 results for: cel.2

Showing: 1301 - 1350 of 12031 results

  1. pαH-S-GSAS-OMICRON

    Plasmid
    #180423
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available Since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    180423-plasmid-map-sequence-id-354264

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  2. PLOD2-bio-His

    Plasmid
    #51756
    Purpose
    Expresses full-length Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    PLOD2 (PLOD2 Human)
    Use
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Aug. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    51756-plasmid-map-sequence-id-98759

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  10. NSP3C-Flag

    Plasmid
    #210349
    Purpose
    Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells
    Depositor
    Michele Pagano
    Article
    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1
    Insert
    C terminal of SARS-CoV-2 NSP3 (ORF1ab )
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Many synonymous changes due to codon optimization
    Promoter
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    210349-plasmid-map-sequence-id-418173

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  11. pαH-S-GSAS-2P-N317P

    Plasmid
    #196378
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein with mutation N317P with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Manrique et al iScience. 2023 Jan 20;26(1):105855. doi:
    Insert
    S-GSAS-2P-N317P (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV 3C cleavage site (before tags) C terminal on …
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available Since
    March 24, 2023
    Availability
    Academic Institutions and Nonprofits only

  23. p1.1-Tr2-eGFP

    Plasmid
    #162782
    Purpose
    Fluorescent reporter for protein expression studies
    Depositor
    Ivan Vorobiev
    Article
    Sinegubova et al PLoS One. 2021 Feb 2;16(2):e0242890. doi
    Insert
    enhanced green fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    162782-plasmid-map-sequence-id-338334

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  46. MDH2_EC

    Plasmid
    #224146
    Purpose
    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.
    Depositor
    Katherine Walstrom
    Insert
    MDH-2 (mdh-2 Nematode)
    Use
    Tags
    His tag, Xpress tag, enterokinase cleaveage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224146-plasmid-map-sequence-id-444697

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  48. pLD-puro-Cc-SARS-CoV-2_Spike_Alpha-VA

    Plasmid
    #191217
    Purpose
    Lentiviral expression of SARS-CoV-2 Spike_Alpha in mammalian cells
    Depositor
    Mohan Babu
    Insert
    SPIKE_SARS2_Alpha (S SARS CoV-2)
    Use
    Lentiviral
    Tags
    VA tag
    Expression
    Mammalian
    Mutation
    Del 69-70 Del 144 N501Y A570D D614G P681H T716I S…
    Promoter
    CMV
    Available Since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191217-plasmid-map-sequence-id-422113

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Showing: 1301 - 1350 of 12031 results