We narrowed to 1,987 results for: ENDOG

1. qTAG-KO-PGK-Puro

   Plasmid

   #227264

   Purpose

   Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Halo-WIPI2 HRD

   Plasmid

   #207544

   Purpose

   Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous WIPI2 locus in human cells

   Depositor

   Jens Schmidt

   Article

   Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

   Insert

   HaloTag flanked by human WIPI2 locus sequences
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Endogenous

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAc5 HA3-eDHFR-T2A-puro

   Plasmid

   #86395

   Purpose

   for C-terminal tagging with eDHFR trimethoprim regulated degron with puro selection

   Depositor

   David Bentley

   Article

   Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

   Insert

   HA3 eDHFR T2A puro
   

   Use

   CRISPR

   Tags

   HA3 eDHFR T2A puro

   Expression

   Mutation

   R12Y, G67S and Y100I mutations in E coli DHFR

   Promoter

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMC_mClover3_BSDminus_F0

   Plasmid

   #184165

   Purpose

   Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Inserts

   mClover3
   bsd
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tornado-RNA sensor for MALAT-1

   Plasmid

   #185406

   Purpose

   circular RNA-scaffolded sensing modules for MALAT-1

   Depositor

   Jian-Hui Jiang

   Article

   Zhou et al J Am Chem Soc. 2021 Sep 8;143(35):14394-

   Insert

   circular RNA-scaffolded sensing modules
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   U6

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-TO-Sp dCas9-mVenusN

   Plasmid

   #191396

   Purpose

   SpdCas9 fused to N-terminal part of mVenus

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   N-terminal part of split mVenus
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 Flag-MBD1-14-mVenusC

   Plasmid

   #191393

   Purpose

   MBD domain of MBD1 fused to C-terminal part of split mVenus

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   pcDNA3 Flag-MBD1-14-mVenusC
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc5 HA3-eDHFR-T2A-neo

   Plasmid

   #86396

   Purpose

   for C-terminal tagging with eDHFR trimethoprim regulated degron with neo selection

   Depositor

   David Bentley

   Article

   Sheridan et al Biotechniques. 2016 Feb 1;60(2):69-74. d

   Insert

   HA3 eDHFR T2A neo
   

   Use

   CRISPR

   Tags

   HA3 eDHFR T2A neo

   Expression

   Mutation

   R12Y, G67S and Y100I mutations in E coli DHFR

   Promoter

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACUH-sfcherry2(1-10)

   Plasmid

   #172061

   Purpose

   UAS vector to express sfcherry2(1-10) in Drosophila

   Depositor

   Daichi Kamiyama

   Article

   Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   sfcherry2(1-10)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1a-mTagBFP-T2A-TetOn3G

    Plasmid

    #179888

    Purpose

    Expresses TetOn3G transcription factor selectable by hygromycin resistance (Lentiviral vector)

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    TetOn3G
    

    Use

    Lentiviral

    Tags

    mTagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMC_mClover3_BSDminus_F2

    Plasmid

    #184167

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMC_mClover3_BSDminus_F1

    Plasmid

    #184166

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lv EF1a HP1cs-Frb1x PGK Puro

    Plasmid

    #102808

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb1x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    HP1cs-Frb1x
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. plenti-arRNA-BFP-backbone

    Plasmid

    #180221

    Purpose

    For the cloning of arRNA-expressing vectors containing BFP fluorescence marker in mammalian cells

    Depositor

    Wensheng Wei

    Article

    Qu et al Nat Biotechnol. 2019 Sep;37(9):1059-1069

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 Ras-LOCKR-S R89L (negative control)

    Plasmid

    #216497

    Purpose

    FRET-based reporter for Ras activity, untargeted, negative control

    Depositor

    David Baker

    Article

    Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

    Insert

    Ras-LOCKR-S R89L negative control
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. th-P2A-Gal4 donor

    Plasmid

    #65563

    Purpose

    Intron targeting-mediated Gal4 knockin at the zebrafish th locus.

    Depositor

    Jiu-lin Du

    Article

    Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

    Insert

    th-P2A-Gal4 (th Zebrafish)
    

    Use

    Zebrafish injection

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Halo-ATG9A HRD

    Plasmid

    #203222

    Purpose

    Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    HaloTag flanked by human ATG9 locus sequences
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFSW FLEx Synapsin-FLAG

    Plasmid

    #139653

    Purpose

    Conditional expression of Synapsin-FLAG

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    Synapsin-FLAG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    June 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX459-HypaCas9-AAVS1_sgRNA

    Plasmid

    #183891

    Purpose

    pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lv EF1a HP1cs-Frb2x PGK Puro

    Plasmid

    #102809

    Purpose

    Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb2x fusion protein

    Depositor

    Jerry Crabtree

    Article

    Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

    Insert

    HP1cs-Frb2x
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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