We narrowed to 21,470 results for: SPR

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pSTBlue-U6-sgRNA(tdTomato)

Plasmid

#47871

Purpose

This plasmid contains expression cassette for sgRNA targeting tdTomato.

Depositor

Erik Sontheimer, James Thomson

Article

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Insert

sgRNA-tdTomato

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pYS16

Plasmid

#202082

Purpose

Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in

Depositor

Daniel Dickinson

Article

Shi et al Development. 2023 May 15;150(10):dev2013

Insert

CT mNG-T2A-PuroR Minicircle Parent

Use

CRISPR

Tags

mNeonGreen

Promoter

None

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NADP-Snifit

Plasmid

#113913

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NADP-Snifit

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJZC595

Plasmid

#62322

Purpose

Expresses MCP-VP64 and dCas9 in Yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Inserts

MCP-VP64
dCas9

Tags

VP64

Expression

Yeast

Mutation

Has nuclease-inactivating mutations in the RuvC1 …

Promoter

Tdh3 and pAdh

Available Since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pWS176

Plasmid

#90963

Purpose

Cas9 gap repair vector - ZeoR

Depositor

Tom Ellis

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJG85

Plasmid

#89281

Purpose

Entry vector containing the TaU6 promoter and an Esp3I Golden Gate cloning site for cloning of the sgRNA, all between attL5 and attL2 sites

Depositor

Daniel Voytas

Article

Gil-Humanes et al Plant J. 2016 Dec 10. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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EC70364

Plasmid

#209515

Purpose

Cloning in complete guide expression cassettes, with Cas9

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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EC67842

Plasmid

#209516

Purpose

Cloning in complete guide expression cassettes, with Cas12a (D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ145-ZmUbi-tRNA

Plasmid

#158403

Purpose

Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAs

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Maize ubiquitin 1

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCF525-AcrIIA4_gb39_lenti

Plasmid

#138305

Purpose

Lenti expression of AcrIIA4

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

AcrIIA4

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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pColE1_sgRNA_NT_AmpR

Plasmid

#119144

Purpose

Encodes non-targeting sgRNA

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

non-targeting sgRNA

Use

Crispr

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_GABPA_2

Plasmid

#64255

Purpose

Expresses gRNA against human GABPA along with Cas9 with 2A GFP

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA

Use

CRISPR

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK-DN4

Plasmid

#180285

Purpose

KanR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - KanR - CEN6/ARS4

Expression

Bacterial and Yeast

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ269E2

Plasmid

#164721

Purpose

Gateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V04 for C-T base editing

Depositor

Yiping Qi

Article

Ren et al Plant Biotechnol J. 2021 May 27. doi: 10

Insert

A3A(Y130F)-zCas9(D10A)-UGI-T2A-MS2-UGI

Use

CRISPR; Gateway compatible a3a/y130f-cbe_v04 entr…

Expression

Plant

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-Ccdc134-IRES-BFP

Plasmid

#117266

Purpose

Retroviral overexpression of Ccdc134

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

Ccdc134 (Ccdc134 )

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200)

Plasmid

#119857

Purpose

Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) (YTHDF1 100-200 subdomain)

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-NYTHDF1(100-200)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200)

Plasmid

#119856

Purpose

Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200) (YTDHF2 100-200 subdomain)

Depositor

Bryan Dickinson

Article

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Insert

dCas13b-GGS-NYTHDF2(100-200)

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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gh92

Plasmid

#106763

Purpose

expression of gRNA targeting FUT8

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

FUT8 (FUT8 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLib6.4-Aalb_743

Plasmid

#176662

Purpose

Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJYScr

Plasmid

#132719

Purpose

Plasmid for an easy-to-construct crRNA delivery vector using hybridized oligonucleotides. Constitutive transcription of crRNA, Spectinomycin resistance

Depositor

Jan Marienhagen

Article

Krumbach et al ACS Synth Biol. 2019 Dec 11. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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