We narrowed to 10,723 results for: SEC

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pBig1a zz TEV YBBR TPP1 MBP TEV TIN2

Plasmid

#185446

Purpose

Coexpresses human TPP1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site and human TIN2 with an MBP affinity tag and TEV cleavage site in insect cells

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Inserts

TPP1 (TPP1 Human)
TIN2 (TINF2 Human)

Tags

MBP, YBBR, and ZZ

Expression

Insect

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFL His-p97

Plasmid

#113510

Purpose

Expression of His-p97 in insect cells (Baculovirus system)

Depositor

Hemmo Meyer

Article

Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

Insert

p97 (VCP Human)

Tags

6*His

Expression

Insect

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pFastbac1-GST-hUSP7

Plasmid

#63573

Purpose

Expression of synthetic human USP7 tagged with GST

Depositor

Titia Sixma

Article

Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

Insert

USP7 (USP7 Human, Synthetic)

Tags

GST

Expression

Insect

Mutation

Synthetic based on human protein sequence (please…

Promoter

Polyhedrin

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_neomycin

Plasmid

#65311

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

STIM1 mutated for binding motif to microtubule (STIM1 Human)

Tags

streptavidin

Expression

Mammalian

Mutation

2 amino acids of STIM1 (IP from SxIP motif) were …

Promoter

CMV

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-ORP8-H514A-H515A

Plasmid

#208360

Purpose

Mammalian expression of fluorescent N-terminally tagged H514A-H515A mutant of ORP8

Depositor

Paula Nunes-Hasler

Article

Criado Santos et al Commun Biol. 2023 Oct 4;6(1):1008. doi:

Insert

ORP8 (OSBPL8 Human)

Tags

EGFP

Expression

Mammalian

Mutation

H514A-H515A

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pLIB_GST-TEV-SRC(WT)

Plasmid

#223742

Purpose

Expression of recombinant protein for purification, codon optimized for Insect cells

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

Src Kinase (SRC Human)

Tags

GST-TEV

Expression

Insect

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCasper4-g-osk-TRICK

Plasmid

#71244

Purpose

oskar translation biosensor with 12x copies of modified PP7 stem loops and 6xMS2 stem loops. . full genomic region, used for P element-mediated germline transformation in Drosophila.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

oskar (osk Fly)

Use

P element-mediated germline transformation

Tags

12xPP7 and 6xMS2

Expression

Insect

Promoter

osk

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-αIFNα-ib

Plasmid

#110798

Purpose

Expression of an intrabody derived from the monoclonal antibody 4EA1 against all mouse interferon α subtypes in mammalian cells

Depositor

Thomas Böldicke

Article

Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do

Insert

intrabody against mouse interferon alpha

Tags

ER retention signal (SEKDEL), ER signal peptide, …

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.JARID2

Plasmid

#125166

Purpose

Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

JARID2 (JARID2 Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pOGS539_rPD-L1nb

Plasmid

#226717

Purpose

Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Recombinant PD-L1 nanobody

Tags

HA tag, Histidine tag, and Mating factor alpha se…

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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FRB-GFP-Gab2(Y604F/Y634F)

Plasmid

#188659

Purpose

FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

GRB associated binding protein 2 (Gab2 Mouse)

Tags

FRB and GFP

Expression

Mammalian

Mutation

Y604F and Y634F

Promoter

CMV

Available Since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pUAS-Myc-BirA-G3-ER

Plasmid

#173815

Purpose

Promiscuous biotin ligase (BirA-G3) targeted to ER lumen expressed from UAS enhancer. N-terminal secretion signal from Drosophila BiP. C-terminal KDEL. Myc tag inserted after sig. seq. cleavage site

Depositor

Norbert Perrimon

Article

Droujinine et al Nat Commun. 2021 Apr 22;12(1):2382. doi:

Insert

Myc-BirA-G3-ER

Tags

Myc

Expression

Insect

Promoter

UAS

Available Since

Aug. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Dom neg CstF64

Plasmid

#127250

Purpose

Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

CstF64 delta 282 (CSTF2 Human)

Use

Other

Expression

Mammalian

Mutation

insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…

Promoter

pEF1a

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pIE-TGFB2-H6

Plasmid

#128500

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (TGFB2 Human)

Tags

His tag (6x)

Expression

Insect

Promoter

IE1

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EPOP

Plasmid

#125170

Purpose

Expresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

EPOP (EPOP Human)

Tags

His-MBP

Expression

Insect

Promoter

Polyhedrin

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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JpExpress404 PTEN

Plasmid

#49420

Purpose

Bacterial Expression Vector

Depositor

Ramon Parsons

Article

Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

Insert

PTEN (PTEN )

Expression

Bacterial

Promoter

T7

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pFB1.HMBP.PrS.dEZH2

Plasmid

#220241

Purpose

Expression of an EZH2 catalytic site mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

dEZH2 (EZH2 Human)

Tags

6x His, MBP

Expression

Insect

Mutation

Y731F

Promoter

Polyhedrin

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBacI-KDAC8 H143A

Plasmid

#224344

Purpose

Expresses human KDAC8 (HDAC8) H143A in insect cells

Depositor

Terry Watt

Article

Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

Insert

KDAC8 (HDAC8 Human)

Tags

TEV-cleavable His6

Expression

Insect

Mutation

Histidine 143 mutated to alanine

Promoter

Polyhedrin

Available Since

Oct. 22, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac SpyTag-β2AR-SpyTag

Plasmid

#135620

Purpose

Expression of SpyTag-B2AR in insect cell

Depositor

Hee-Jung Choi

Article

Choi et al Science. 2019 Nov 29;366(6469):1150-1156

Insert

Beta 2 adrenergic receptor (ADRB2 Human)

Tags

HA-FLAG tag, Spytag, and eGFP-10xHis-R1D4 tag

Expression

Insect

Mutation

Deleted 1-24 aa, 366-413 aa

Available Since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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PF3D7_1136200-bio

Plasmid

#47730

Purpose

Expresses enzymatically monobiotinylated full-length PF3D7_1136200 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised PF3D7_1136200

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin replaced…

Promoter

CMV

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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