We narrowed to 18,127 results for: Mut

1. H2B-LSSmGFP

   Plasmid

   #186539

   Purpose

   Localization of LSSmGFP fluorescent protein (ex/em = 400/510 nm) to histone 2B

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP
   

   Use

   Tags

   Human Histone 2B (D26G and V119I mutations)

   Expression

   Mammalian

   Mutation

   hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K

   Promoter

   CMV

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pICH51288

   Plasmid

   #50269

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. V5-TurboID-Mfn1E209A

   Plasmid

   #195156

   Purpose

   mammalian expression of TurboID tagged Mfn1E209A

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   TurboID
   

   Use

   Tags

   Mfn1-E209A

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   CMV

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CF-PIPKi

   Plasmid

   #20158

   Depositor

   Tobias Meyer

   Article

   Suh et al Science. 2006 Dec 1. 314(5804):1454-7.

   Insert

   CFP-FKBP-PIPK (D253A) (Pip5k1c Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D253A mutation in PIPK. C-terminus (amino acids …

   Promoter

   Available Since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. hRIP3 GFP D160N

   Plasmid

   #41386

   Depositor

   Francis Chan

   Article

   Cho et al Cell. 2009 Jun 12;137(6):1112-23.

   Insert

   RIP3 (RIPK3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Kinase Domain Mutant (D160N)

   Promoter

   CMV

   Available Since

   Dec. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-L1-PGK-HygTK-L2

   Plasmid

   #141006

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   HygTK
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. EcMBP165-cpGFP.PPYF.T203V.pRSET

   Plasmid

   #33373

   Purpose

   a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

   Depositor

   Loren Looger

   Article

   Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

   Insert

   Improved MBP based Maltose Biosensor
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   cpGFP146 variant from GCaMP2 inserted between AA1…

   Promoter

   T7

   Available Since

   Dec. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTKhc-yibDp-GFP-cp6

   Plasmid

   #134938

   Purpose

   Low phosphate inducible circular permutation of GFP

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   GFPcp6
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFG036 + pFG051

   Plasmid

   #137997

   Purpose

   Transposon mutagenesis plasmid and the cI repressor expression plasmid

   Depositor

   Sébastien Rodrigue

   Article

   Neil et al Commun Biol. 2020 Sep 22;3(1):523. doi:

   Insert

   Tn5 tnp
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA4-Myc-HisA-H248Y-Sirt3

    Plasmid

    #24917

    Depositor

    Toren Finkel

    Article

    Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

    Insert

    Sirt3 (SIRT3 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    His248 mutated to Tyr(Y)

    Promoter

    Available Since

    June 10, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pECFP-DEVG-Venus

    Plasmid

    #24538

    Depositor

    Peter Sorger

    Article

    Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

    Insert

    ECFP-DEVG-Venus
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    DEVDR mutated to DEVG

    Promoter

    CMV

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pCD/NL-BH*deltavpu/RT-

    Plasmid

    #136985

    Purpose

    Second-generation lentiviral packaging plasmid lacking reverse transcriptase activity

    Depositor

    Jakob Reiser

    Article

    Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

    Insert

    HIV-1 Gag, Pol (Integrase), Tat, Rev, Vpr, Vif
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    carries D110E mutation in the reverse transcripta…

    Promoter

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

    Plasmid

    #159912

    Purpose

    Mutagenesis of Kcnd3

    Depositor

    Larry Zweifel

    Insert

    Kcnd3 (Kcnd3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. BFP-Rab5CA (Q79L)

    Plasmid

    #205705

    Purpose

    Mammalian expression of BFP-tagged mutant Rab5CA

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    RAB5A (RAB5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q79L

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FRB-myc

    Plasmid

    #20228

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    T2098L point mutation

    Promoter

    Available Since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pMUH_unc84_tdTFlag

    Plasmid

    #46024

    Depositor

    Sean Eddy

    Article

    Henry et al Nucleic Acids Res. 2012 Oct;40(19):9691-

    Insert

    UNC-84 (unc-84 )
    

    Use

    10xuas-phi31-expression vector

    Tags

    tdTomato

    Expression

    Mutation

    Two amino acid mutations (F279L and L500P) compar…

    Promoter

    10XUAS

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HI.fate.SFFV-E

    Plasmid

    #191555

    Purpose

    Monitor HIV-1 fate

    Depositor

    Alon Herschhorn, Joseph Sodroski

    Article

    Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

    Insert

    HIV-1 incomplete genome
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    env-deleted + vpr mutation(s)

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HI.fate.E

    Plasmid

    #191553

    Purpose

    Monitor HIV-1 fate

    Depositor

    Alon Herschhorn, Joseph Sodroski

    Article

    Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

    Insert

    HIV-1 incomplete genome
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    env-deleted + vpr mutation(s)

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HI.fate.UB

    Plasmid

    #191556

    Purpose

    Monitor HIV-1 fate

    Depositor

    Alon Herschhorn, Joseph Sodroski

    Article

    Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

    Insert

    HIV-1 incomplete genome
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    env-deleted + vpr mutation(s)

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HI.fate.SFFV

    Plasmid

    #191554

    Purpose

    Monitor HIV-1 fate

    Depositor

    Alon Herschhorn, Joseph Sodroski

    Article

    Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

    Insert

    HIV-1 incomplete genome
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    env-deleted + vpr mutation(s)

    Promoter

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJMK004

    Plasmid

    #34616

    Purpose

    Viral expression of Arch (D95N mutant with greater sensitivity but slower response) voltage indicator

    Depositor

    Adam Cohen

    Article

    Kralj et al Nat Methods. 2011 Nov 27. doi: 10.1038/n

    Insert

    Arch D95N
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Aspartic Acid 95 to Asparagine

    Promoter

    CaMKII

    Available Since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. hSDH5_G78R

    Plasmid

    #113047

    Purpose

    Expression of mutant hSDH5

    Depositor

    Edward A. Berry

    Article

    Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

    Insert

    hSDH5_G78R (SDHAF2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    G78R, Insert begins at amino acid 39

    Promoter

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. BiP-mGFP P495L

    Plasmid

    #62232

    Purpose

    Mutant of hamster BiP-mGFP unable to bind clients.

    Depositor

    Erik Snapp

    Article

    Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

    Insert

    hamster BiP-mGFP-KDEL
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available Since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTRE-Bi-SG-T

    Plasmid

    #26084

    Depositor

    Liqun Luo

    Article

    Li et al PLoS One. 2010 . 5(7):e11503.

    Insert

    Synaptophysin (Syp Mouse)
    

    Use

    Tags

    mut4EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. hSDH5_C83D

    Plasmid

    #113048

    Purpose

    Expression of mutant hSDH5

    Depositor

    Edward A. Berry

    Article

    Zafreen et al Arch Biochem Biophys. 2016 Aug 15;604:47

    Insert

    hSDH5_C83D (SDHAF2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C83D, Insert begins at amino acid 39

    Promoter

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEB1-mCerulean3

    Plasmid

    #103973

    Purpose

    Plasmid encoding mCerulean3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mCerulean3
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, Y66W, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pmax-3xFLAG_3xNLS_dPspCas13b(AAAA)_2xNLS-Clover

    Plasmid

    #196829

    Purpose

    Express dpdpCas13b with AAAA mutation and Clover

    Depositor

    Albert Cheng

    Article

    Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

    Insert

    dpspCas13b(AAAA)
    

    Use

    Tags

    3XFLAG and NES

    Expression

    Mammalian

    Mutation

    K367A, D369A and K370A

    Promoter

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. YIplac211-Sec31-yoHalo

    Plasmid

    #115423

    Purpose

    Express Sec31-yoHalo at the endogenous locus via pop-in pop-out mutagenesis

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    Sec31-yoHalo
    

    Use

    Tags

    yoHaloTag

    Expression

    Yeast

    Mutation

    Promoter

    Endogenous

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pScDD2_ERdd

    Plasmid

    #109047

    Purpose

    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).

    Depositor

    Thomas Wandless

    Insert

    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    

    Use

    Tags

    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…

    Expression

    Yeast

    Mutation

    3 mutations : E380G, R434G, N532S

    Promoter

    SOD1

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MAC_N_STAT4

    Plasmid

    #167802

    Purpose

    Expresses STAT4 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    STAT4 (STAT4 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJL2-mGold-P2A-EBFP2

    Plasmid

    #157998

    Purpose

    Co-expression of mGold and EBFP2 in mammalian cells.

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    mGold-P2A-EBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mGold is mVenus with L46F;T63S mutations

    Promoter

    CMV

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEB2-DsRed-Express

    Plasmid

    #103995

    Purpose

    Plasmid encoding DsRed-Express

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    DsRed-Express
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMSP2

    Plasmid

    #26282

    Depositor

    Stephen Sligar

    Article

    Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

    Insert

    MSP2
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    synthetic gene of dimer of deletion mutant (1-43)…

    Promoter

    Available Since

    April 7, 2011

    Availability

    Academic Institutions and Nonprofits only

34. Wnt4 STOP

    Plasmid

    #35873

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt4 (WNT4 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Silent Mutation C (492) --> T

    Promoter

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pENTR-L1-PGK-TKNeo-L2

    Plasmid

    #141008

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    TKNeo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GR A458T

    Plasmid

    #133410

    Purpose

    overexpression of a mutant human glucocorticoid receptor in mammalian cells

    Depositor

    Andrew Cato

    Article

    Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

    Insert

    NR3C1 (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A458T

    Promoter

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. saposin B^W

    Plasmid

    #161759

    Purpose

    Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

    Depositor

    Sam Gellman

    Article

    Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

    Insert

    saposin B R38_W
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    changed Arginine 38 to Tryptophan

    Promoter

    T7

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. LV anti-FITC-LaG16-Y186F-T2A-BFP

    Plasmid

    #194931

    Purpose

    LV construct encoding Y186F mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCAG-postASAP-p2a-ChrimsonR

    Plasmid

    #178794

    Purpose

    Expresses voltage sensor targeted to dendrites and spines along with ChrimsonR

    Depositor

    Rafael Yuste

    Article

    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

    Insert

    FingR.PSD95, ASAP2f and ChrimsonR
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    mutations in amino acids L146G, S147T N149R, S150…

    Promoter

    CAG

    Available Since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBP-SJM901

    Plasmid

    #72966

    Purpose

    Level 0 - SJM901 promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    SJM901 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge