We narrowed to 11,283 results for: VARS

1. pTwist-SARS-CoV-2 Δ18 B.1.351v2

   Plasmid

   #169463

   Purpose

   Encodes SARS-CoV-2 B.1.351v2 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.351v2 Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB-cT3G-cERP2-WT1

   Plasmid

   #192906

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of WT1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   WT1 (WT1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTwist-SARS-CoV-2 Δ18 B.1.1.7

   Plasmid

   #169459

   Purpose

   Encodes SARS-CoV-2 B.1.1.7 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

   Insert

   SARS-CoV-2 B.1.1.7 Spike truncated to remove 18 amino acids (S )
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-cT3G-cERP2-PPARG

   Plasmid

   #192912

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of PPARG

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   PPARG (PPARG Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.1 mitoAURKA

   Plasmid

   #157750

   Purpose

   Expression of AURKA targeted to mitochondria

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Expression

   Mammalian

   Mutation

   Aurora A without the first 30AA + MTS of CoxVIII

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-SpCas9-HF1-P2A-EGFP (RTW3505)

   Plasmid

   #139995

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9-HF1(N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9-HF1 with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   HF1=N497A/R661A/Q695A/Q926A

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pαH-S-GSAS

   Plasmid

   #164565

   Purpose

   SARS-CoV-2 Spike protein (S-GSAS variant)

   Depositor

   Priyamvada Acharya

   Article

   Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

   Insert

   Spike (S-GSAS variant)
   

   Tags

   2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

   Expression

   Mammalian

   Mutation

   Ectodomain (AAs 1-1208); 682-685 (furin site = RR…

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. MS2_adRNA-MCP_ADAR2 DD_NES

   Plasmid

   #124706

   Purpose

   Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD_NES

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   MCP_ADAR2 DD_NES (RAB7A Human)
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MS2_adRNA-MCP_ADAR1 DD_NES

   Plasmid

   #124630

   Purpose

   Expresses MS2_adRNA targeting the RAB7A transcript along with MCP_ADAR1 DD_NES

   Depositor

   Prashant Mali

   Article

   Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

   Insert

   MCP_ADAR1 DD_NES (RAB7A Human)
   

   Use

   AAV

   Tags

   NES

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

    Plasmid

    #106204

    Purpose

    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.A184S
    

    Use

    AAV

    Mutation

    GltI: A184S + mRuby3 fusion

    Promoter

    CAG

    Available Since

    Feb. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-RR-ABE8e-V106W

    Plasmid

    #193656

    Purpose

    Expression of base editor RR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence

    Depositor

    Liangxue Lai

    Article

    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

    Insert

    dLbCas12a-TadA*8e-V106W, EGFP
    

    Expression

    Mammalian

    Mutation

    dLbCas12a-G532R/K595R, TadA*8e-V106W

    Promoter

    CMV

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB-cT3G-cERP2-NR1H2

    Plasmid

    #192919

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of NR1H2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    NR1H2 (NR1H2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    TET3G-miniCMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-U6-DR30(SapI) 0.5 Syn-intron-hfCas13d-pA

    Plasmid

    #233039

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a 0.5 bp synapsin promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and hfCas13d
    

    Use

    AAV

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT2-TetR-neoR

    Plasmid

    #153310

    Purpose

    Tet-repressor with neomycin selection marker, to be used together with pT2-CMV/TetO2-EYFP-GW

    Depositor

    Spyros Petrakis

    Article

    Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

    Insert

    TetR
    

    Use

    Transposon

    Promoter

    SV40

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHH0103_BCAR1-1/1

    Plasmid

    #91418

    Purpose

    Protein expression and purification of human SH3 domain construct BCAR1-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    BCAR1-1/1 (BCAR1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX VAMP7 (1-188)

    Plasmid

    #42315

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

    Insert

    VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Δ181-220

    Promoter

    Ptac

    Available Since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pHH0103_FYN-1/1

    Plasmid

    #91413

    Purpose

    Protein expression and purification of human SH3 domain construct FYN-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    FYN-1/1 (FYN Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

    Plasmid

    #119723

    Purpose

    AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

    Depositor

    Thomas Oertner

    Article

    Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

    Inserts

    CaMPARI2
    CaMPARI2
    

    Use

    AAV

    Tags

    NES-his

    Expression

    Mammalian

    Mutation

    F391W, L398V

    Promoter

    hSyn1

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDF-Az

    Plasmid

    #197101

    Purpose

    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    

    Expression

    Bacterial

    Mutation

    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

    Available Since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pαH-S-RRAR/D614G

    Plasmid

    #164570

    Purpose

    SARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike (S-RRAR-D614G variant)
    

    Tags

    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…

    Expression

    Mammalian

    Mutation

    D614G

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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