162,160 results

1. pHR_SFFv_Her2-BFP_RHL027

   Plasmid

   #164823

   Purpose

   Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Insert

   Extracellular and transmembrane region of ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   tagBFP

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEX-CMV-SP-YFP-STM2(15-746)

   Plasmid

   #18862

   Depositor

   Tobias Meyer

   Article

   Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

   Insert

   Stromal interaction molecule 2 (STIM2 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   YFP inserted between signal peptide(SP) of STIM1 …

   Promoter

   Available since

   March 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAM5406

   Plasmid

   #132661

   Purpose

   RSF1010-based broad host-range plasmid with additional RK2 bom site and pUC origin

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3` CC: Hygro – GFP-N – loxP – mScarlet

   Plasmid

   #219564

   Purpose

   3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosome

   Depositor

   Andrea Ventura

   Article

   Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

   Insert

   3` CC: Hygro – GFP-N – loxP – mScarlet
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   EF1-a

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAL204

   Plasmid

   #22282

   Purpose

   Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   pCMV-PhyB(1-908)-10aaLinker-mCFP-Kras4BCT (PHYB Mustard Weed)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYS16

   Plasmid

   #202082

   Purpose

   Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   CT mNG-T2A-PuroR Minicircle Parent
   

   Use

   CRISPR

   Tags

   mNeonGreen

   Expression

   Mutation

   Promoter

   None

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-G-Rac1

   Plasmid

   #180575

   Purpose

   ddGFP sensor of Rac1

   Depositor

   Won Do Heo

   Article

   Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

   Insert

   ddGFP sensor of Rac1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CL20_mEGFP_YAP1_MAMLD1

   Plasmid

   #205955

   Purpose

   Express mEGFP-tagged fusion protein, YAP1_MAMLD1 from patient-derived sequence

   Depositor

   Richard Kriwacki

   Article

   Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

   Insert

   YAP1_MAMLD1 (YAP1, MAMLD1 Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiRNAGuide_002 - hU6-RfxCas13d-DR-BsmBI-EFS-Puro-WPRE

   Plasmid

   #138151

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   hU6_Rfxcas13d-DR_BsmBI
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-Tpr

    Plasmid

    #35024

    Depositor

    Larry Gerace

    Article

    Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30.

    Insert

    Tpr (TPR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRE-mCherry-p2A-bReaches-TS

    Plasmid

    #158701

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    mCherry-p2A-bReaches-TS
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTET GFP10-FRB/FKBP-GFP11

    Plasmid

    #182497

    Purpose

    Tripartite split-GFP. E. coli vector. Inducible coexpression of GFP10-FRB and FKBP-GFP11 coiled coils

    Depositor

    Stéphanie Cabantous

    Article

    Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

    Inserts

    FRB
    FKBP (Fkbp1a Mouse, Synthetic)
    

    Use

    Tags

    split GFP10 tripartite and split GFP11 tripartite

    Expression

    Bacterial

    Mutation

    Promoter

    TET

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF5/FRT/DEST-3xHA/mGli3/Flag

    Plasmid

    #51246

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pRF-SrpR

    Plasmid

    #49372

    Depositor

    Christopher Voigt

    Article

    Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

    Insert

    SrpR repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-AAV-syn-FLEX-jGCaMP8f-WPRE

    Plasmid

    #162379

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV and Cre/Lox

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pK7WGF2::hCas9

    Plasmid

    #46965

    Purpose

    Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    hCas9
    

    Use

    CRISPR; Plant expression

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    35S

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPaint-mNeon

    Plasmid

    #174092

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    mNeonGreen
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. EMTB-mCherry

    Plasmid

    #26742

    Depositor

    William Bement

    Article

    Miller et al Nat Cell Biol. 2009 Jan . 11(1):71-7.

    Insert

    ensconsin (MAP7 Human)
    

    Use

    Probe for microtubules

    Tags

    2x mCherry and Myc

    Expression

    Mammalian

    Mutation

    Microtubule binding-domain of ensconsin (amino ac…

    Promoter

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-tdTomato-CNA35

    Plasmid

    #61606

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    tdTomato and CNA35
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. TVBB C-term-mNeongreen-Puro

    Plasmid

    #169228

    Purpose

    Targeting vector backbone to support a knock-in of Linker-mNeongreen-P2A-Puro at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked mNeongreen-P2A-Puro
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. RGB-S reporter

    Plasmid

    #207841

    Purpose

    A three-colour stress biosensor for real time analysis of physiological stress, genotoxicity, and cytotoxicity of Escherichia coli

    Depositor

    Christof Niemeyer

    Article

    Zoheir et al NPJ Biofilms Microbiomes. 2023 Aug 21;9(

    Inserts

    RpoH sensing construct
    SOS sensing construct
    RpoS sensing construct
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PosmY, PsulA, and grpE

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pATetO 6XHis-Taq DNA Pol

    Plasmid

    #178782

    Purpose

    Inducible expression of Taq DNA polymerase in E. coli

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    Taq DNA Polymerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-21a(+)/MBP-His-PINK1

    Plasmid

    #38005

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    PINK1 (PINK1 Human)
    

    Use

    Tags

    6xHIS and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 10, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pLminP_Luc2P_RE52

    Plasmid

    #90397

    Purpose

    STAT1 - STAT1 gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    STAT1

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pmCherry-C1_SEPT9 i1

    Plasmid

    #71622

    Purpose

    mammalian expression of human SEPT9 with an mCherry fusion

    Depositor

    Elias Spiliotis

    Article

    Dolat et al J Cell Biol. 2014 Oct 27;207(2):225-35.

    Insert

    Septin 9 (SEPTIN9 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag

    Plasmid

    #209666

    Purpose

    Non-responsive control

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    cpSFGFP.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. 6xHsa.NFKB:d2EGFP

    Plasmid

    #239990

    Purpose

    Destabilized green fluorescent protein (d2EGFP) under transcriptional control of NF-kB activity

    Depositor

    Raquel Espin-Palazon

    Insert

    Destabilized EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    cfos minimal promoter

    Available since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

    Plasmid

    #87306

    Purpose

    An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

    Depositor

    Li Zhang

    Article

    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre-dependent mouse codon-optimized Flp recombinase.
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMXs-IP HA-Parkin

    Plasmid

    #38248

    Depositor

    Noboru Mizushima

    Article

    Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

    Insert

    Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

    Plasmid

    #171384

    Purpose

    Expression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

    Depositor

    Hui Yang

    Article

    Xu et al Nat Methods. 2021 May;18(5):499-506. doi

    Insert

    humanized dCas13X.1-RESCUE-S
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, U6

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge