We narrowed to 22,836 results for: Sis

1. pJP72

   Plasmid

   #176479

   Purpose

   Expresses mScarlet under control of the Capsaspora EF1 promoter and GenR under control of the Capsaspora actin promoter

   Depositor

   Duojia Pan

   Article

   Phillips et al Elife. 2022 Jun 6;11. pii: 77598. doi: 1

   Inserts

   actin > GenR
   EF1 > mScarlet
   

   Use

   Capsaspora owczarzaki expression

   Available Since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBT296_pBS-U-attP-FRT5-pSV40-Neo-pA-FRT5

   Plasmid

   #52537

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attP (full length)
   Neo
   

   Expression

   Mammalian

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. Halo-WIPI2B L69E

   Plasmid

   #175026

   Purpose

   Expresses WIPI2B (L69E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

   Depositor

   Andrea Stavoe

   Article

   Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

   Insert

   WIPI2B (L69E) (WIPI2 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Leucine 69 to Glutamate (CTA to GAA)

   Promoter

   CMV

   Available Since

   Dec. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBT298_pBS-U-attPx3-FRT5-pSV40-Neo-pA-FRT5

   Plasmid

   #52538

   Purpose

   Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attPx3 (70 bp each)
   Neo
   

   Expression

   Mammalian

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1kb Zfp521 promoter reporter

   Plasmid

   #104188

   Purpose

   luciferase reporter to assay Zfp521 promoter activation

   Depositor

   Kathryn Hentges

   Article

   Yu et al Genesis. 2016 Oct;54(10):519-533. doi: 1

   Insert

   Zfp521 promoter - 1 Kb (Zfp521 Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   none

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHH0103_SASH1-1/1

   Plasmid

   #91347

   Purpose

   Protein expression and purification of human SH3 domain construct SASH1-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SASH1-1/1 (SASH1 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB422

   Plasmid

   #74816

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGWB428

   Plasmid

   #74822

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEPQD1CB0119

   Plasmid

   #177051

   Purpose

   MoClo Level 1, position 1, transcriptional unit for transient expression of major latex protein+H34like (NmMLPL) from Nepeta mussinii driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   major latex protein-like (NmMLPL) from Nepeta mussinii
   

   Use

   Synthetic Biology

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_SLA2-1/1

    Plasmid

    #91450

    Purpose

    Protein expression and purification of human SH3 domain construct SLA2-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SLA2-1/1 (SLA2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHH0103_NCK2-2/3

    Plasmid

    #91362

    Purpose

    Protein expression and purification of human SH3 domain construct NCK2-2/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCK2-2/3 (NCK2 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-HA-RIPK3 S199A

    Plasmid

    #78806

    Purpose

    To overexpress RIPK3 S199A in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S199A

    Promoter

    CMV

    Available Since

    June 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti_CRISPRi_sgNeg2

    Plasmid

    #166999

    Purpose

    Lentiviral expression of negative control sgRNA for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    Negative control sgRNA 2
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pME-hygro-hPGAP3-3HA

    Plasmid

    #50373

    Purpose

    Express C-terminally triple HA tagged human PGAP3 in mammmalian cells

    Depositor

    Reika Watanabe

    Article

    Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

    Insert

    PGAP3 post-GPI attachment to proteins 3 (PGAP3 Human)
    

    Tags

    3x HA

    Expression

    Mammalian

    Promoter

    SR alpha

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pQUAST>stop>shibire (ts1)

    Plasmid

    #24363

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    Shibire (ts1) (shi Fly)
    

    Expression

    Insect

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBGN155-MXMT

    Plasmid

    #79817

    Purpose

    GFP(S65T)-based BiFC vector for plants (Positive control: MXMT)

    Depositor

    Yutaka Kodama, Masamitsu Wada

    Article

    Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

    Insert

    CaMXMT1
    

    Use

    Reporter plasmid

    Tags

    GN155/GFP (1–155)

    Expression

    Plant

    Promoter

    CaMV 35S promoter and CaMV35S

    Available Since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. px330-RPL26 sgRNA2

    Plasmid

    #134642

    Purpose

    contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    RPL26 sgRNA2 (RPL26 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. px330-RPL26 sgRNA1

    Plasmid

    #134641

    Purpose

    contains sgRNA targeting to the C-terminus of human RPL26 for gene editing

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    RPL26 sgRNA2 (RPL26 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGE660

    Plasmid

    #153258

    Purpose

    M4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-HA-mIFITM3-K83,88,104A

    Plasmid

    #58405

    Purpose

    Expresses murine IFITM3-K83,88,104A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lysines 83, 88, and 104 to Alanine

    Promoter

    CMV IE

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pCMV-HA-mIFITM3-K83A

    Plasmid

    #58401

    Purpose

    Expresses murine IFITM3-K83A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lysine 83 to Alanine

    Promoter

    CMV IE

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pCMV-HA-mIFITM3-K24A

    Plasmid

    #58400

    Purpose

    Expresses murine IFITM3-K24A with an N-terminal HA tag in mammalian cells

    Depositor

    Howard Hang, Jacob Yount

    Article

    Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lysine 24 to Alanine

    Promoter

    CMV IE

    Available Since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3-HA-RIPK3 K55R

    Plasmid

    #78807

    Purpose

    To overexpress RIPK3 K55R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K55R

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTrichoGate-13

    Plasmid

    #160713

    Purpose

    For C-terminal tags with Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA) bordered by BsaI restriction sites

    Depositor

    Artemio Mendoza

    Article

    Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

    Insert

    Trichoderma optimised Synthetic 3X Hemagglutinin tag (HA)
    

    Use

    Synthetic Biology

    Available Since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pMHBAD14

    Plasmid

    #42304

    Depositor

    Hideo Iwai

    Article

    Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

    Insert

    NpuDnaE C-intein
    

    Tags

    GB1-H6

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet

    Plasmid

    #83286

    Purpose

    untargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    mTurquoise2-FHA2-AURKB substrate-YPet
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pGFP-Ub-VPS13D_dC

    Plasmid

    #176466

    Purpose

    express VPS13D lacking C-terminal 407 aa in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    C-terminal 407 aa is deleted.

    Promoter

    CMV

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEYFP-N1-hRIP3-FL V460P

    Plasmid

    #61379

    Purpose

    Expresses Human full length RIPK3 with V460P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP3 with V460Pmutation (RIPK3 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed V460 to P

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pEYFP-N1-hRIP3-FL V458P

    Plasmid

    #61377

    Purpose

    Expresses Human full length RIPK3 with V458P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP3 with V458Pmutation (RIPK3 Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed V458 to P

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

30. kank2_pJet1.2

    Plasmid

    #121980

    Purpose

    for generating kank2 riboprobes for WISH in zebrafish

    Depositor

    GuangJun Zhang

    Article

    Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

    Insert

    kank2 (LOC571549 Zebrafish)
    

    Use

    Cloning vector

    Promoter

    T7

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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31. EGFP-bSV1_1-1009

    Plasmid

    #129471

    Purpose

    Mammalian expression of the N-terminus + central domain of bovine SV1

    Depositor

    Elizabeth Luna

    Article

    Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

    Insert

    Supervillin (SVIL Bovine)
    

    Expression

    Mammalian

    Mutation

    contains amino acids 1-1009

    Promoter

    CMV

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJK526

    Plasmid

    #155387

    Purpose

    PphlF-TA4 crRNAs, 1x cascade. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    PphlF-TA4 in dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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33. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)

    Plasmid

    #61939

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-156)
    

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 156

    Promoter

    T7

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

34. UKNP4.3.1

    Plasmid

    #98269

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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35. UKNP3.2.2

    Plasmid

    #98247

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHH0103_NCK1-1/3

    Plasmid

    #91472

    Purpose

    Protein expression and purification of human SH3 domain construct NCK1-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    NCK1-1/3 (NCK1 Human)
    

    Expression

    Bacterial

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRSET FLIPglu-600uDelta11 Ares-Aphrodite

    Plasmid

    #13571

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

    Insert

    FLIPglu F16A Delta11 Aphrodite
    

    Tags

    Aphrodite and Ares

    Expression

    Bacterial

    Mutation

    Fluorophore-derived linker shortened. F16A mutati…

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA5/FRT/TO DNAJB7

    Plasmid

    #19476

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB7 (DNAJB7 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEG302 22aa SunTag NtDRMcd SUP

    Plasmid

    #115489

    Purpose

    CRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAs

    Depositor

    Steven Jacobsen

    Article

    Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

    Insert

    g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBP-ORF

    Plasmid

    #72949

    Purpose

    Entry vector for ORF's - PCR, gene synthesis or sub-clone using NdeI/BamHI. Alternatively use BsaI with CATA and TCGA fusion sites. Stop codon must be included upstream of chosen 3' restriction site

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 0 - ORFs
    

    Use

    Synthetic Biology

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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