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We narrowed to 2,478 results for: EFS

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Showing: 1081 - 1120 of 2478 results

  14. pCSIIbsr-Raichu-Rab5/PM

    Plasmid
    #214819
    Purpose
    Encoding a Förster resonance energy transfer (FRET) biosensor for Rab5 activity with cyan and yellow fluorescent protein.
    Depositor
    Michiyuki Matsuda
    Article
    Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:
    Insert
    A Förster resonance energy transfer (FRET) biosensor for Rab5 activity with cyan and yellow fluorescent protein.
    Expression
    Mammalian
    Promoter
    EF-1α
    Available Since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    214819-plasmid-map-sequence-id-427357

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  22. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

    Plasmid
    #70658
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    March 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. BirA*-HA-NES

    Plasmid
    #214473
    Purpose
    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.
    Depositor
    Natalie Ahn
    Article
    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.
    Insert
    BirA(R118G)
    Use
    Lentiviral; Gateway
    Tags
    HA tag and NES (Nuclear Exclusion Sequence)
    Expression
    Mammalian
    Mutation
    R118G
    Promoter
    EF-1a
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214473-plasmid-map-sequence-id-425592

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  33. lentiCRISPR - Amplicon, BCR/ABL sgRNA 1

    Plasmid
    #70659
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone
    Depositor
    David Sabatini
    Article
    Wang et al Science. 2015 Oct 15. pii: aac7041.
    Insert
    sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    70659-plasmid-map-sequence-id-162503

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  35. pRDA_103

    Plasmid
    #133460
    Purpose
    H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance.
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133460-plasmid-map-sequence-id-261409

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  37. pFC482

    Plasmid
    #246439
    Purpose
    The plasmid encodes a transcriptional unit for the expression of the CasE endoribonuclease fused with a nuclear localization signal
    Depositor
    Ron Weiss
    Article
    Caliendo et al Nat Chem Biol. 2024 Sep 12. doi: 10.1038
    Insert
    CasE
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SapI domesticated
    Promoter
    EF-1a
    Available Since
    Nov. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    246439-plasmid-map-sequence-id-492325

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Showing: 1081 - 1120 of 2478 results