We narrowed to 10,745 results for: AGA

1. CROP_C9_Puro_FLT3

   Plasmid

   #183293

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets FLT3 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   FLT3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOC2 Dlg4-Halo-FKBP KI

   Plasmid

   #183435

   Purpose

   CreOFF knock-in for PSD95-Halo-FKBP (amino acid position: R721)

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   gRNA and Halo-FKBP donor
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGWB100

   Plasmid

   #68062

   Purpose

   MCS-containing binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pM115: CasRx control presgRNA

   Plasmid

   #166867

   Purpose

   U6-driven expression of control (non-targeting) presgRNA compatible with CasRx.

   Depositor

   Guei-Sheung Liu

   Article

   Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

   Insert

   non-targeting presgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV/3' Box_(GLuc)_TOP2

   Plasmid

   #68435

   Purpose

   Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   TOP2 construct
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6_(GLuc)_INT[Spinach2]

   Plasmid

   #68430

   Purpose

   Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct_bearing the "Spinach2" aptamer
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lsg-LRRK2-1

   Plasmid

   #199277

   Purpose

   nicking sgRNA to induce LRRK2 (c. 6055 G > A) mutation using PE3

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   LRRK2 nick
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBJ1807 pRS415-COF1RFP

   Plasmid

   #37102

   Depositor

   John Cooper

   Article

   Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

   Insert

   COF1-RFP
   

   Use

   Tags

   RFP

   Expression

   Yeast

   Mutation

   Single RFP internal insertion at Cof147T48D with …

   Promoter

   Available since

   July 13, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pWKS1583

   Plasmid

   #70188

   Purpose

   Modular vector for secreted amylase reporter expression

   Depositor

   Wiep Klaas Smits

   Article

   Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

   Insert

   amyEopt
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. Str-KDEL_flGalT-SBP-tagBFP

    Plasmid

    #65274

    Purpose

    synchronize trafficking of GalT from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    galactosidase
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCK671.306

    Plasmid

    #192641

    Purpose

    Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    J306 scRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23119

    Available since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFUSE-rabbitFc1-IL2ss-VHH(B12)-His-Myc

    Plasmid

    #209837

    Purpose

    Plasmid coding for a nanobody against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1

    Depositor

    Tim Brown

    Inserts

    IL2-ss
    VHH (B12 variant)
    Rabbit Fc Gamma 1
    

    Use

    Tags

    x3 MycTag and x6 HisTag

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1-HTLV promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPR_eGFP_314

    Plasmid

    #111822

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 294-314
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBJ1808 pRS416-COF1RFP

    Plasmid

    #37103

    Depositor

    John Cooper

    Article

    Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

    Insert

    COF1-RFP
    

    Use

    Tags

    RFP

    Expression

    Yeast

    Mutation

    Single RFP internal insertion at Cof147T48D with …

    Promoter

    Available since

    June 19, 2012

    Availability

    Academic Institutions and Nonprofits only

15. lentiCRISPR - DDX3Y sgRNA 2

    Plasmid

    #70657

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pGL4.11 promPDE4D3 Ebox mut

    Plasmid

    #27304

    Depositor

    David E. Fisher

    Article

    Khaled et al Genes Dev. 2010 Oct 15. 24(20):2276-81.

    Insert

    prom PDE4D3 (PDE4D Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Ebox CACATG changed to GAGATG

    Promoter

    Available since

    Jan. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAF256

    Plasmid

    #105496

    Purpose

    pRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and LgBiT

    Depositor

    Wiep Klaas Smits

    Article

    Oliveira Paiva et al J Mol Biol. 2019 Jan 8. pii: S0022-2836(

    Insert

    HupA-SmBiT/LgBiT
    

    Use

    Atc-dependent expression in c. difficile

    Tags

    Expression

    Mutation

    Promoter

    Ptet

    Available since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pbs/pU6-HDAC4 RNAi

    Plasmid

    #21100

    Depositor

    Connie Cepko

    Article

    Chen et al Science. 2009 Jan 9. 323(5911):256-9.

    Insert

    RNAi against histone deacetylase4 (HDAC4 Mouse, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pAc-sgRNA-Cas9.scr_2

    Plasmid

    #190704

    Purpose

    Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Scrambled sgRNA 2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. MXS_CMV::NeoR-bGHpA

    Plasmid

    #62437

    Purpose

    MXS_chaining vector with CMV::NeoR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Neomycin with CMV Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. px458_2A_GFP_sgRNA_NEMF

    Plasmid

    #127126

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    gRNA NEMF (NEMF Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUC19-U6-crRNA4n96

    Plasmid

    #123640

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    crRNA scaffold 4n96
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pOC2 Dlg4-Halo KI

    Plasmid

    #183449

    Purpose

    CreOFF knock-in for PSD95-Halo (amino acid position: R721)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and Halo donor
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCfB5191

    Plasmid

    #126911

    Purpose

    Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    X-4 and XII-5 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. lentiCRISPR - DDX3Y sgRNA 1

    Plasmid

    #70656

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and a DDX3Y targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Oct 15. pii: aac7041.

    Insert

    sgRNA against DDX3Y
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

26. Lenti-sgRbm10/Cre

    Plasmid

    #89648

    Purpose

    Expresses an Rbm10-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Rbm10 (Rbm10 Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MXS_PGK::PuroR-bGHpA

    Plasmid

    #62440

    Purpose

    MXS_chaining vector with PGK::PuroR-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    resistance cassette against Puromycin with PGK Promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pU6_(GLuc)_TOP1

    Plasmid

    #68423

    Purpose

    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    TOP1 construct
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CROP_C9_Puro_VEGFB

    Plasmid

    #183328

    Purpose

    All-in-One CRISPRko system with a guide RNA that targets VEGFB gene

    Depositor

    Prashant Mali

    Article

    Hu et al Biomaterials. 2022 Jan;280:121276. doi:

    Insert

    VEGFB
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDONR221-OSF-TRIM5-21R

    Plasmid

    #79063

    Purpose

    Gateway entry vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5-21R
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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