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We narrowed to 43,766 results for: tra

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Showing: 5641 - 5660 of 43766 results

  6. pEB1N-LZ-mCherry-LOV2(wt)

    Plasmid
    #107693
    Purpose
    N-terminal half of π-EB1 with wild-type LOV2, mCherry-tagged to track microtubule ends
    Depositor
    Torsten Wittmann
    Article
    van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038
    Insert
    MAPRE1 (MAPRE1 Human)
    Tags
    A. sativa phototropin 1 LOV2 domain, GCN4 leucine…
    Expression
    Mammalian
    Mutation
    EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    107693-plasmid-map-sequence-id-209378

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  8. pVQ CMV NanoV1Mutant-2a-EGFP ferritin

    Plasmid
    #79650
    Purpose
    Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein
    Depositor
    Jeffrey Friedman
    Article
    Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do
    Inserts
    transient receptor potential vanilloid 1 (Trpv1 Rat)
    camelid anti-GFP nanobody
    Enhanced green fluorescent protein
    mouse ferritin light chain (Ftl1 Mouse)
    mouse ferritin heavy chain (Fth1 Mouse)
    Use
    Adenoviral
    Expression
    Mammalian
    Mutation
    isoleucine 679 changed to lysine
    Promoter
    CMV
    Available Since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    79650-plasmid-map-sequence-id-156910

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  18. dCas9-CBP FL

    Plasmid
    #179550
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoter
    Depositor
    Isaac Hilton
    Article
    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78
    Insert
    S. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
    Use
    Lentiviral
    Tags
    Flag Tag
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EF-1alpha
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    179550-plasmid-map-sequence-id-354775

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  19. pcDNA5/FRT/TO-GAlphai3-RLuc8

    Plasmid
    #140975
    Purpose
    Encodes a G alpha subunit (GNAI3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphai3-RLuc8 (GNAI3 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140975-plasmid-map-sequence-id-300658

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  20. pcDNA5-EGFP-NLS-P2AT2A-mCherry-PTS1

    Plasmid
    #87828
    Purpose
    High-efficient mammalian expression vector for co-expression of EGFP-tagged and mCherry-tagged proteins using P2AT2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    NLS
    PTS1
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available Since
    April 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    87828-plasmid-map-sequence-id-175012

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Showing: 5641 - 5660 of 43766 results