We narrowed to 17,521 results for: Emb
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Plasmid#88846PurposeCRISPR KO of Trp53DepositorAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only
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Px461-Cas9n-Trp53-sgRNA-beta
Plasmid#88847PurposeCRISPR KO of Trp53DepositorAvailable SinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLV_3XHA-ATP6V1B2-mNeonGreen
Plasmid#228865PurposeStable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreenDepositorAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF.STAT3DN.Ubc.GFP
Plasmid#24984PurposeDepositorInsertSTAT3 (STAT3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationY705F--dominant negativeAvailable SinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNeonGreen-C1-Nir1-LNS2
Plasmid#220083PurposeHigh affinity PA biosensor with green fluorophore (also known as NeonGreen-PILS-Nir1)DepositorInsertPITPNM3 (PITPNM3 Human)
TagsNeonGreen-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-EF1a-CreERT2-3Xflag-T2A-eBFP2
Plasmid#170186PurposeThis Cre-ERT2 expressing construct can be used to inducibly recombine loxp sites. It can be used with poly-loxP containing plasmids to generate timestamp barcodes useful for linage tracingDepositorInsertCreERT2-T2A-eBFP2
UseLentiviralPromoterEF1 alphaAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-PTEN
Plasmid#116866PurposeRecruitable PTEN dual-specificity phosphataseDepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRT_htr2c(c)-GFP
Plasmid#79677Purposeserotonin receptor minigene with consensus splice sites that expresses the full length serotonin receptor with a C-terminal EGFPDepositorInsertserotonin receptor 2C (HTR2C Human)
TagsEGFP in FrameExpressionMammalianMutationsplice site of exon Vb in consensusPromoterCMVAvailable SinceAug. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
YY206: pAAV_hSyn::GEMINI(B)-p2A-GEMINI(A)_UbC::HaloTag-GEMINI(A)
Plasmid#228886PurposeAAV plasmid expressing the two GEMINI building blocks (A and B) in an equimolar ratio under the control of a hSyn promoter, and HaloTag-GEMINI(A) under the control of a UbC promoter.DepositorInsertGEMINI(A); GEMINI(B); HaloTag-GEMINI(A)
UseAAVTagsHaloTagExpressionMammalianPromoterhSyn; UbcAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pME-EGFP-P2A-iCre (JDW 921)
Plasmid#224519PurposeA Gateway compatible middle entry clone containing EGFP P2A iCre (codon optimized cre)DepositorInsertEGFP-P2A-iCre
UseCre/LoxAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
OMM-Flag-APEX2-SOPP3
Plasmid#229073PurposeExpresses APEX2-SOPP3 targeted to outer mitochondrial membraneDepositorInsertExpress APEX2-SOPP3 in outer mitochondrial membrane (APEX2 Human)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Gal3
Plasmid#85662PurposeFluorescent reporter for lysosomal damageDepositorAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Strep-FLAG KBP
Plasmid#236459Purposetransient overexpression of KBP in mammalian cellsDepositorAvailable SinceMay 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
JL054: pPB_NFκB::GFP-GEMINI(A)-P1N4_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR
Plasmid#228884PurposePiggyBac plasmid expressing the destabilized GFP-GEMINI(A) under the control of a synthetic NFκB-responsive promotor, and rtTA3 and PuroR under the control of a UbC promoter.DepositorInsertGFP-fused GEMINI(A), HaloTag-fuse GEMINI(A)
ExpressionMammalianPromoterpNFκB; TREAvailable SinceFeb. 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
pDG459
Plasmid#100901PurposeSpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.DepositorInsertshumanized CRISPR associated protein 9
U6-gRNA scaffold 1
U6-gRNA scaffold 2
UseCRISPR and Mouse TargetingTags3xFLAG and T2A-PuroRExpressionMammalianPromoterCBh and U6Available SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-CAAX
Plasmid#86056PurposeA mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.DepositorInsertHomo sapiens Harvey rat sarcoma (HRAS Human)
TagsGFPExpressionMammalianMutationcontains the region from 825-857 of HRAS as a far…PromoterCMVAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
mClY-N1
Plasmid#90457PurposeA YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.DepositorInsertmClY-N1
ExpressionMammalianMutationEYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…Available SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-hATG2A
Plasmid#114462PurposeExpresses human ATG2A with GFP in mammalian cellsDepositorInserthuman ATG2A (ATG2A Human)
UseRetroviralTagsGFPExpressionMammalianMutationP656R (please see depositors comment below)PromoterCMVAvailable SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-FLAG-Jhdm2a
Plasmid#38136DepositorInsertJhdm2a (Kdm3a Mouse)
TagsFLAGExpressionMammalianMutationprotein starts at aa115PromoterCMVAvailable SinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
FSW CHIP-Myc
Plasmid#246724PurposeNeuron-specific expression of human CHIP wildtype, and a myc-tag on its C-terminusDepositorInsertCHIP (Carboxy terminus of Hsc70-interacting protein) (STUB1 Human)
UseLentiviralTagsMycExpressionMammalianPromoterSynapsinAvailable SinceMarch 3, 2026AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRL1 CTF-FLAG
Plasmid#217706PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL1 (ADGRL1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-838Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRB3 CTF-FLAG
Plasmid#217683PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRB3 (BAI3 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-856Available SinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-Luciferase-WPRE-bGH-pA (JDW 1509)
Plasmid#242572PurposeGateway 3' entry clone containing an IRES LuciferaseDepositorInsertIRES-Luciferase-WPRE-bGH-pA
UseGateway subcloningAvailable SinceSept. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-EGFP-miR-MCS (JDW 1231)
Plasmid#242562PurposeGateway middle entry clone containing an EGFP fused to a microRNA multiple cloning site.DepositorInsertEGFP with artifial intron and MCS
UseGateway subcloningAvailable SinceSept. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME TAEL2.0-5xC120-minpro (JDW 1109)
Plasmid#224507PurposeGateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)DepositorInsertTAEL2.0-5xC120
UseGateway cloningAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-mCherry-hPMCA2w/b
Plasmid#111568PurposeExpresses a plasma membrane calcium pump hPMCA2w/bDepositorHas ServiceAAV5InsertPlasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
UseAAVTagsmCherryAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mStayGold-TOMM20
Plasmid#227307PurposeDonor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)DepositorInsertTOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME Actin Vhh mNeonGreen-HA-pA (JDW 1248)
Plasmid#224500PurposeGateway compatible middle entry clone containing Actin nanobody fused to mNeonGreen with an HA tag (Visualizing actin, actin chromobody)DepositorInsertActin-Vhh-mNeonGreen-HA-pA
UseGateway cloningAvailable SinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-dr-myl7-pro (JDW 1148)
Plasmid#229836PurposeA gateway compatible 5' entry clone containing the mysoin light chain seven (myl7/cmlc2) promoter for cardiomyocyte specific expression.DepositorInsertcmlc2/myl7
UseGateway entry cloneAvailable SinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-C1-Nir1-LNS2
Plasmid#220084Purposehigh affinity PA biosensor with red fluorophore (also known as mCherry-PILS-Nir1)DepositorInsertPITPNM3 (PITPNM3 Human)
TagsmCherry-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable SinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)
Plasmid#224536PurposeA Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3DepositorInsertIRES-FLAG- NLS-mScarlet-I3
UseGateway cloningAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-zFUCCI (JDW 1464)
Plasmid#242558PurposeGateway middle entry clone containing zebrafish FUCCI reporter; For visualizing cell cycle state.DepositorInsertmCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)
UseGateway subcloningAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL-SBP-EGFP-LAMP1
Plasmid#222317PurposeSynchronize the trafficking of LAMP1 from the ER.DepositorInsertStreptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)
ExpressionMammalianPromoterCMVAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-Actin-Vhh-mNeonGreen-HA-pA (JDW 1308)
Plasmid#242589PurposeA CAGGS driven expression vector containing an actin nanobody fused to mNeonGreen to label actin.DepositorInsertActin-Vhh (actin nanobody)
ExpressionMammalianPromoterCAGGSAvailable SinceSept. 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2/DEST/hTfR-HA
Plasmid#69610Purposehuman transferrin receptor with an HA tag fused at the C-terminusDepositorAvailable SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-RIGI-Apex- T2A-EGFP
Plasmid#167288PurposeLentiviral expression plasmid encoding an APEX2 RIG-I fusion product in frame with a self-cleaving eGFP. APEX biotin ligase can biotynilate RNAs in proximity of the RLR RIG-I sensor.DepositorAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-MFN2-Strep (SB253)
Plasmid#227602PurposeInducible expression of Strep-tagged MFN2 in Pichia pastorisDepositorAvailable SinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-EGFP-CAAX
Plasmid#208683PurposeExpresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cellsDepositorInsertmembrane-localized enhanced green fluorescent protein EGFP-CAAX
ExpressionMammalianPromoterCMVAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PATagRFP-GRP78
Plasmid#238419PurposeExpresses GRP78 fused to the photoactivatable Tag-red fluorescent protein (PATagRFP-GRP78)DepositorAvailable SinceSept. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-LMNB1
Plasmid#207770PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.DepositorInsertsgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only