We narrowed to 10,333 results for: rras

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pAT253

Plasmid

#40943

Depositor

Angela Taddei

Article

Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

Insert

GFP-LacI**

Tags

GFP(S65T, V163A) and SV40 NLS

Expression

Yeast

Mutation

LacI**/LacI2G(4 mutations) (see comment below); l…

Promoter

His3 promoter fragment

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pETDuet RagA-RagC

Plasmid

#99641

Purpose

Bacterial expression of wildtype Rag GTPase dimer

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Dec. 28, 2017

Availability

Academic Institutions and Nonprofits only

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PLKO-mcherry-luc-puro-Renilla cDNA

Plasmid

#29783

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

Renilla

Use

Lentiviral

Expression

Mammalian

Available Since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

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trPAGmEos3.2

Plasmid

#203752

Purpose

Encodes the transmembrane domain from PAG/CSK, including its 2 palmitoylation sites, with mEos3.2 fused on the C terminus. Used as a fluorescent plasma membrane probe.

Depositor

Sarah Veatch

Article

Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

Insert

trPAG (PAG1 Human)

Tags

mEos3.2

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-BLU-VIPR

Plasmid

#220497

Purpose

Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).

Depositor

Alexander Espinosa

Article

Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pY027

Plasmid

#84742

Purpose

Expresses huLbCpf1 and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Insert

huLbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pBABE-SSeCKs

Plasmid

#106908

Purpose

full-length murine SSeCKs/AKAP12/Gravin in retroviral vector

Depositor

Heidi Hehnly

Article

Colicino et al Mol Biol Cell. 2017 Dec 27. pii: mbc.E17

Insert

SSeCKs (Akap12 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

gag

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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mCit-4aa-SNAP25B

Plasmid

#53235

Purpose

Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

SNAP-25B (Snap25 Rat)

Tags

4aa-linker and mCitrine

Expression

Mammalian

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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mito-CIBN-GFP

Plasmid

#89480

Purpose

Encodes for a fusion protein between a mitochondrial anchor and the CIBN-GFP protein.

Depositor

Xavier Trepat Guixer

Article

Valon et al Nat Commun. 2017 Feb 10;8:14396. doi: 10

Insert

mito-CIBN-GFP (CIB1 Human, Mustard Weed)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Plasmid

#159808

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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766 p3E-2A-nlsmCherrypA

Plasmid

#195972

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-nlsmCherry (nuclear)

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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EKARdual

Plasmid

#99870

Purpose

Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

Depositor

Marc Tramier

Article

Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

Insert

EKAR

Tags

ShadowG and mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pEND-446_Cacnes_ProA

Plasmid

#225609

Purpose

Suicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pZCS38 (Prsp-27::NeoR::unc-54 3’ UTR)

Plasmid

#193049

Purpose

Encodes G-418 resistance

Depositor

Patrick Phillips

Article

Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

Insert

prsp-27::NeoR::unc-54 3’ UTR

Expression

Worm

Promoter

prsp-27

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pENTRY-GFP

Plasmid

#15301

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

hrGFP

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
Opto-beta2AR

Plasmid

#106063

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-beta2AR; Opto-beta2AR; F5; 716)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-beta2AR

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

Plasmid

#124493

Purpose

Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipYFP(TEV cleavage seq) T2A mCherry

Use

Synthetic Biology

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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mCer-4aa-VAMP2

Plasmid

#53233

Purpose

Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

VAMP2 (Vamp2 Rat)

Tags

4aa-linker and mCerulean

Expression

Mammalian

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
pGL3-24p3/LCN2-Luc

Plasmid

#25463

Depositor

Sarah Gaffen

Article

Shen et al J Leukoc Biol. 2005 Mar . 77(3):388-99.

Insert

24p3/lipocalin 2 proximal promoter (Lcn2 Mouse)

Use

Luciferase

Mutation

none

Available Since

June 25, 2010

Availability

Academic Institutions and Nonprofits only
FAB(ER-LD)

Plasmid

#214424

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

FRB-V5

Tags

CFAST-Halo and RspAN-FRB-V5

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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