Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

174,739 results

Showing: 11441 - 11480 of 174739 results

  2. Hs.NRAS

    Plasmid
    #83173
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway Cloning
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83173-plasmid-map-sequence-id-158136

    Enlarge

  8. Synapsin sHRPb-NLG in FSW lentiviral vector

    Plasmid
    #73148
    Purpose
    Small fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPb-NLG (Nlgn1 Synthetic, Mouse)
    Use
    Lentiviral
    Tags
    V5 tag
    Expression
    Mammalian
    Promoter
    Synapsin
    Available Since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73148-plasmid-map-sequence-id-134528

    Enlarge

  17. OPRD1-Tango

    Plasmid
    #66461
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    OPRD1 (OPRD1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66461-plasmid-map-sequence-id-417858

    Enlarge

  18. HTR2B-Tango

    Plasmid
    #66410
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    HTR2B (HTR2B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    66410-plasmid-map-sequence-id-272927

    Enlarge

  23. TetO-FUW-VdC9BV

    Plasmid
    #62195
    Purpose
    Expresses RNA-Guided, Nuclease-Inactive VP64:dCas9-BFP:VP64—VdC9BV—Fusion Protein to Enable Transactivation of Endogenous Genes
    Depositor
    Kam Leong
    Article
    Chakraborty et al Stem Cell Reports. 2014 Dec 9;3(6):940-7
    Insert
    VP64dCas9BFPVP64
    Use
    Lentiviral
    Tags
    Two VP64s tagged to dCas9 fused to BFP
    Expression
    Mammalian
    Mutation
    D10A H840A (catalytically inactive) Cas9 (dCas9)
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62195-plasmid-map-sequence-id-101643

    Enlarge

  30. mDsRed-Golgi-7

    Plasmid
    #55832
    Purpose
    Localization: Golgi, Excitation: 557, Emission: 585
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Tags
    mDsRed
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    55832-plasmid-map-sequence-id-494617

    Enlarge

  31. THBS1-bio-His

    Plasmid
    #53417
    Purpose
    Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    THBS1 (THBS1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53417-plasmid-map-sequence-id-83207

    Enlarge

  34. pSBtet-RN

    Plasmid
    #60503
    Purpose
    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    TCE & RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60503-plasmid-map-sequence-id-97544

    Enlarge

  36. mPA-GFP-LaminB1-10

    Plasmid
    #57141
    Purpose
    Localization: Nuclear Envelope, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    LaminB1 (LMNB1 Human)
    Tags
    mPA-GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  38. mEGFP-H4-23

    Plasmid
    #56463
    Purpose
    Localization: Nucleus/Histones, Excitation: 488, Emission: 507
    Depositor
    Michael Davidson
    Insert
    H4 (HIST1H4J Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 23, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    56463-plasmid-map-sequence-id-244945

    Enlarge

  39. tdTomato-TOMM20-N-10

    Plasmid
    #58137
    Purpose
    Localization: Outer Mitochondrial Membrane, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    TOMM20 (TOMM20 Human)
    Tags
    tdTomato
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  40. pBUN6A11

    Plasmid
    #50579
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    OsU3p and Ubi1p
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50579-plasmid-map-sequence-id-75626

    Enlarge

Showing: 11441 - 11480 of 174739 results