164,223 results

1. U6-sgH11-eCas9-T2A-BlastR

   Plasmid

   #172492

   Purpose

   For CRISPR-assisted HDR, Hipp11 locus

   Depositor

   Tyler Jacks

   Article

   Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

   Insert

   , BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus
   

   Use

   CRISPR, Lentiviral, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCMV_WNV_PrME

   Plasmid

   #207313

   Purpose

   Mammalian expression of West Nile Virus PrME protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   WNV_PrME (POLY West Nile Virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-TBG-ER-TurboID

   Plasmid

   #149415

   Purpose

   Hepatocyte-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

   Depositor

   Jonathan Long

   Article

   Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

   Has Service

   AAV8

   Insert

   ER-TurboID
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mutation

   Promoter

   TBG

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hsyn-GRAB_DA2m

   Plasmid

   #140553

   Purpose

   Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   The 2nd generation DA sensor GRAB_DA2m (medium affinity)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Flag-HA-USP37

   Plasmid

   #22602

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP37 (USP37 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available Since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBait

   Plasmid

   #165146

   Purpose

   Bait plasmid for PROBER (empty backbone encoding replaceable ccdB cassette)

   Depositor

   Paul Khavari

   Article

   Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYPQAT56

   Plasmid

   #223428

   Purpose

   T-DNA vector for dSpCas9 mediated gene activation for dicot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   ZmUbi-dSpCas9-Act3.0-AtU3-gRNA scaffold 2.0
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFS181

   Plasmid

   #12536

   Depositor

   Nick Rhind

   Article

   Sivakumar et al Methods. 2004 Jul . 33(3):213-9.

   Insert

   human equilibrating nucleoside transporter 1 (SLC29A1 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9 sgRNA vector

   Plasmid

   #68463

   Purpose

   Cas9 sgRNA vector for cloning

   Depositor

   Su-Chun Zhang

   Article

   Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-3xFLAG-V5-ccdB

    Plasmid

    #87064

    Purpose

    Mammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tag

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

    Type

    Empty backbone

    Use

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAB1678 CMV-HIVNES-GS-Cas13bt1

    Plasmid

    #176316

    Purpose

    CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

    Depositor

    Feng Zhang

    Article

    Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

    Insert

    human codon optimized Cas13bt1
    

    Use

    CRISPR

    Tags

    HIV NES

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBI-CMV1-mir144-CMV2-mir1

    Plasmid

    #164634

    Purpose

    Express miR-144 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    mir144 (MIR144 Human)
    mir-1-1 (MIR1-1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCF525-AcrIIA4_gb39_lenti

    Plasmid

    #138305

    Purpose

    Lenti expression of AcrIIA4

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    AcrIIA4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. TDP-REGv2(Gaussia Luciferase)#5 in pTwist-CMV

    Plasmid

    #216164

    Purpose

    Expression of secreted Gaussia Luciferase specifically in cells with TDP-43 loss of function. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    Gaussia princeps luciferase (with double mutation to improve stability)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP_Rab8a

    Plasmid

    #31803

    Depositor

    Daniel Gerlich

    Article

    Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

    Insert

    Rab8a (RAB8A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

16. 1436 pcDNA3 Flag HA

    Plasmid

    #10792

    Purpose

    pcDNA3 vector with added Flag, HA tags

    Depositor

    William Sellers

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMC_mClover3_BSDminus_F2

    Plasmid

    #184167

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMC_mClover3_BSDminus_F1

    Plasmid

    #184166

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGGF_RT_FAST_RFP

    Plasmid

    #136979

    Purpose

    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

    Depositor

    Klaus Theres

    Article

    Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

    Insert

    seed coat marker RFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

    Promoter

    Available Since

    Jan. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHCMV_BoDV1_G

    Plasmid

    #207256

    Purpose

    Mammalian expression of Borna disease virus 1 G protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    BoDV1_G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-hSyn-GRAB_r5-HT1.0

    Plasmid

    #208719

    Purpose

    Expresses the red 5-HT sensor GRAB_r5-HT1.0 in neurons

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Red fluorescent 5-HT sensor GRAB_r5-HT1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available Since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. His-GFP-Clathrin

    Plasmid

    #170858

    Purpose

    Mammalian expression of His-GFP-Clathrin.

    Depositor

    Justin Taraska

    Article

    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

    Insert

    Clathrin light polypeptide (Lca) (Clta Mouse)
    

    Use

    Tags

    (His)6 and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUAS-V5-TurboID-NES

    Plasmid

    #116904

    Purpose

    Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

    Depositor

    Norbert Perrimon

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Nrp1-Fc-His

    Plasmid

    #72097

    Purpose

    Expresses the extracellular region of the Neuropilin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Nrp1 (Nrp1 Mouse)
    

    Use

    Tags

    Fc-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEBB HA cIAP1

    Plasmid

    #38232

    Depositor

    Colin Duckett

    Article

    Csomos et al Biochem J. 2009 Apr 28;420(1):83-91.

    Insert

    cIAP1 (BIRC2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET32a-LIC

    Plasmid

    #62310

    Purpose

    E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis, S-tag, and Trx-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available Since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSEVA3b67Rb

    Plasmid

    #58311

    Purpose

    Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.

    Depositor

    Tom Ellis

    Article

    Wright et al ACS Synth Biol. 2014 May 13.

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAd/CMV/V5-Clasp2α

    Plasmid

    #89763

    Purpose

    Adenovirus destination vector for transient expression of human wild-type EGFP-CLASP2α

    Depositor

    Torsten Wittmann

    Article

    Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

    Insert

    CLASP2α (CLASP2 Human)
    

    Use

    Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDEST/LIfeAct-mCherry-N1

    Plasmid

    #40908

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    LifeAct
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    No stop codon to create c-terminal fusion

    Promoter

    CMV

    Available Since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV-U6-sgRNA-hSyn-mCherry

    Plasmid

    #87916

    Purpose

    sgRNA expressing AAV construct with a mCherry reporter driven by hSyn promoter. (replaced the GFP in pX552 from Zhang lab with mCherry)

    Depositor

    Alex Hewitt

    Article

    Hung et al Invest Ophthalmol Vis Sci. 2016 Jun 1;57

    Insert

    mCherry
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUC57-gCarvi-T2A

    Plasmid

    #190333

    Purpose

    Fluorescent reporter for cAMP

    Depositor

    Naoto Saitoh

    Article

    Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

    Insert

    gCarvi
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRK5-HA-Ubiquitin-K6

    Plasmid

    #22900

    Depositor

    Sandra Weller

    Article

    Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

    Insert

    Ubiquitin C (UBC Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K6 only, all other lysines mutated to arginines

    Promoter

    Available Since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-Dio-GFP

    Plasmid

    #198279

    Purpose

    Cre reporter for mammalian cells; double-floxxed-EGFP

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    Dio-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGL3-cMYC promoter

    Plasmid

    #114724

    Purpose

    reporter luciferase construct

    Depositor

    Martine Roussel

    Article

    Roussel et al Oncogene. 1994 Feb;9(2):405-15.

    Insert

    C-Myc promoter region
    

    Use

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PBCAG-EGFR

    Plasmid

    #40974

    Depositor

    Joseph Loturco

    Article

    Chen et al J Neurosci Methods. 2012 Jun 15;207(2):1

    Insert

    EGFR (EGFR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Flag-cIAP2/pRK5

    Plasmid

    #27973

    Depositor

    Xiaolu Yang

    Article

    Hu et al J Clin Invest. 2006 Jan . 116(1):174-81.

    Insert

    CIAP2 (BIRC3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLentiCMV Puro DEST p38KTRmCerulean3

    Plasmid

    #59155

    Purpose

    Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFPN3-Sstr3

    Plasmid

    #35623

    Depositor

    Kirk Mykytyn

    Article

    Berbari et al Mol Biol Cell. 2008 Apr;19(4):1540-7. Ep

    Insert

    Sstr3 (Sstr3 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. CAG-mCherry-sesRNA-GFP-WPRE-pA

    Plasmid

    #192065

    Purpose

    Expression of mCherry, sesRNA in mammalian cells, with GFP as efRNA.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for Cheta
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAG1186 Lyn11-ECFP-FIREmate

    Plasmid

    #205168

    Purpose

    Expresses Lyn11-ECFP-FIREmate in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    Lyn11-ECFP-FIREmate
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge