We narrowed to 54,588 results for: plasmid

1. pJAT39

   Plasmid

   #204289

   Purpose

   For marking CRISPR HDR integrant with EGFP expressed in flight muscles.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-EGFP+H3:H26
   

   Use

   CRISPR

   Promoter

   MHC

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAM5411

   Plasmid

   #132663

   Purpose

   RSF1010-based broad host-range plasmid with RK2 bom site and pUC origin expressing YFP and Sp/Sm resistance

   Depositor

   Susan Golden

   Article

   Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

   Inserts

   aadA
   YFP
   

   Expression

   Bacterial

   Promoter

   PaadA and PconII

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE.puro/TO/Flag/HuR.wt

   Plasmid

   #110346

   Purpose

   Doxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG tag

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   gag

   Available Since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE.puro/TO/Flag/tGFP-HuR.wt

   Plasmid

   #110349

   Purpose

   Doxycycline inducible mammalian retroviral expression of HuR (wild type) with FLAG and turboGFP tags

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Retroviral

   Tags

   FLAG and tGFP

   Expression

   Mammalian

   Promoter

   gag

   Available Since

   Nov. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNR4A1-donor

   Plasmid

   #112295

   Purpose

   CRISPR donor plasmid to tag human transcription factor NR4A1 with GFP

   Depositor

   Kevin White

   Insert

   NR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
   

   Use

   CRISPR

   Mutation

   hg38:12:52058840:C>T, hg38:12:52058930:A>C,…

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. KSR1-HaloTag Fusion Vector

   Plasmid

   #236794

   Purpose

   Express KSR1-HaloTag(R) Fusion Protein in Mammalian Cells under a CMV promoter

   Depositor

   Promega Corporation

   Has Service

   DNA

   Insert

   KSR1 (KSR1 Human)
   

   Tags

   HaloTag (R)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available Since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pGFP Cas

   Plasmid

   #50729

   Purpose

   mammalian expression of GFP-Cas. Note-This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

   Depositor

   Kenneth Yamada

   Insert

   Cas (Bcar1 Rat)
   

   Tags

   FLAG and GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-GluA2-scFv-mNeon-KDEL

   Plasmid

   #230057

   Purpose

   AAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA2 single chain variable fragment (scFv)
   

   Use

   AAV

   Tags

   ER retrieval motif (KDEL) and mNeon

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. SJZ5-HPH

   Plasmid

   #228258

   Purpose

   Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

   Depositor

   Zhiping Xie

   Article

   Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

   Insert

   hph
   

   Expression

   Yeast

   Promoter

   SSA1 S.c.

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRN1120

    Plasmid

    #101750

    Purpose

    Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBIS-GALkFLP(URA)

    Plasmid

    #49460

    Purpose

    overexpression of Flp step-arrest mutant in yeast, URA selection

    Depositor

    Marc Gartenberg

    Article

    Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

    Insert

    Flp(H305L) (flp Budding Yeast)
    

    Expression

    Yeast

    Mutation

    H305L, ‘step-arrest’ mutant,

    Promoter

    GAL10

    Available Since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. SJZ9-amdS

    Plasmid

    #228261

    Purpose

    Template plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen source

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    amdS
    

    Expression

    Yeast

    Promoter

    ENO2 S.c.

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6xO/S luc

    Plasmid

    #69445

    Purpose

    detection/assessment of OCT4 and SOX2 transcription factor activity

    Depositor

    Lisa Dailey

    Insert

    minimal fgf4 promoter and 6XOS tandem repeat of composite OCT4/Sox2 binding site from FGF4 enhancer
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSP-GM1

    Plasmid

    #64739

    Purpose

    Yeast expression plasmid

    Depositor

    Jens Nielsen

    Article

    Chen et al FEMS Yeast Res. 2012 Aug;12(5):598-607.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLZ12Km2-P23R:TA:ffluc

    Plasmid

    #88900

    Purpose

    Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

    Depositor

    Thomas Proft

    Article

    Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

    Insert

    toxin-antitoxin + firefly luciferase
    

    Use

    Luciferase

    Expression

    Bacterial

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJA252

    Plasmid

    #21512

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    pmex-5 (w/o ATG)
    

    Use

    Multisite gateway

    Available Since

    Aug. 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-GluA1-scFv-mNeon-KDEL

    Plasmid

    #230056

    Purpose

    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    AAV

    Tags

    ER retrieval motif (KDEL) and mNeon

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGBW-m4134100

    Plasmid

    #152050

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHis-003

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pJAT21

    Plasmid

    #204309

    Purpose

    For generating targeting inversions. N-terminal split GFP, expressed in abdomen and mouthparts

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-GFPn
    

    Use

    CRISPR

    Promoter

    ie1

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJAT25

    Plasmid

    #204310

    Purpose

    For generating targeting inversions. C-terminal split GFP, expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-GFPc
    

    Use

    CRISPR

    Promoter

    ie1

    Available Since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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