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Showing: 1401 - 1450 of 18154 results
  1. pBridge-tyrosinase.σ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Inserts
    tyrosinase cytosolic tail
    AP-1 σ1A
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4…
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resi…
    Promoter
    ADH1 and MET25
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. INCTbiosyn-p35S-GFP(rc)phiC31

    Plasmid
    #127527
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase phiC31 attachment sites.
    Depositor
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase phiC31 attachment sites.
    Expression
    Plant
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. INCTbiosyn-p35S-GFP(rc)Bxb1

    Plasmid
    #127528
    Purpose
    Plasmid has a CaMV 35S promoter in a forward sequence orientation and an inverted EGFP coding sequence (reverse complement) flanked by attB and attP integrase Bxb1 attachment sites.
    Depositor
    Insert
    EGFP reverse complement coding sequence flanked by attB/attP Integrase Bxb1 attachment sites.
    Expression
    Plant
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. CROPseq-MPSV-eBFP2

    Plasmid
    #220472
    Purpose
    Expresses either functional CRISPR gRNAs from the hU6-promotor and empowers pairing of CRISPR screens with 3' and 5' scRNA-Seq capturing by FACS-sorting on eBFP2+ without prior resistance selection
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pTripz NEO DEST

    Plasmid
    #209072
    Purpose
    Modified DOX-ON inducible destination vector with a c-terminal 3X flag tag to be used for LR recombination reactions to inducibly express cDNAs of interest
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral; Gateway destination vector to be used…
    Tags
    3X FLAG
    Promoter
    CMV
    Available Since
    Nov. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. pOTTC1784 - pAAV SYN1 hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201820
    Purpose
    An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET imaging
    Depositor
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    AAV
    Promoter
    SYN1
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid
    #169164
    Purpose
    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells
    Depositor
    Insert
    nsp14/nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    6His-3xFlag (nsp10 C-terminus)
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. TOPO mMecp2-NLucTom

    Plasmid
    #159282
    Purpose
    To tag endogenously and c-terminally mouse Mecp2 with two reporters: NanoLuciferase and TdTomato. It contains 2 homology arms from a castaneus background surrounding the STOP codon.
    Depositor
    Insert
    Mecp2 (Mecp2 Mouse)
    Tags
    Nluciferase-P2A-TdTomato
    Expression
    Mammalian
    Mutation
    Nluciferase-TdTomato fusion
    Available Since
    Feb. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pOTTC1822 - pAAV SYN1 DIO hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201821
    Purpose
    An adeno-associated viral vector expressing Cre-dependent channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a synapsin promoter, for use in PET
    Depositor
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    AAV
    Promoter
    SYN1
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  11. pOTTC1845 - pAAV CaMKII hChR2(H134R)-V5-ER-LBD (ChRERa)

    Plasmid
    #201822
    Purpose
    An adeno-associated viral vector expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CaMKii promoter, for use in PET imaging
    Depositor
    Insert
    hChR2(H134R)-V5-ER-LBD (ChRERa)
    Use
    AAV
    Promoter
    CaMKii
    Available Since
    Oct. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  12. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118155
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the hPGK promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    hPGK and U6
    Available Since
    May 6, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. 1435 pSG5L Flag HA

    Plasmid
    #10791
    Purpose
    Mammalian expression empty backbone.
    Depositor
    Type
    Empty backbone
    Tags
    Flag and HA
    Expression
    Mammalian
    Available Since
    Sept. 28, 2005
    Availability
    Academic Institutions and Nonprofits only
  14. pminTolR4-R2

    Plasmid
    #67711
    Purpose
    mini Tol2 backbone with att4-attR2 cassette
    Depositor
    Type
    Empty backbone
    Available Since
    Aug. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
  15. TEF2pro_mCherry_YFP

    Plasmid
    #138623
    Purpose
    Dual reporter backbone plasmid for CCRA experiment
    Depositor
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. TEF1pro_mCherry_YFP

    Plasmid
    #138624
    Purpose
    Dual reporter backbone plasmid for CCRA experiment
    Depositor
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    March 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  17. pIVTR(T7A)-MCS

    Plasmid
    #172292
    Purpose
    Empty backbone for in vitro transcription of mRNA
    Depositor
    Type
    Empty backbone
    Use
    Other
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  18. pIVTR(T7G)-MCS

    Plasmid
    #172287
    Purpose
    Empty backbone for in vitro transcription of mRNA
    Depositor
    Type
    Empty backbone
    Use
    Other
    Available Since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
  19. pTpPBR11_fcpNAT

    Plasmid
    #154035
    Purpose
    Diatom episome backbone for T. pseudonana
    Depositor
    Type
    Empty backbone
    Use
    Diatom cloning
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  20. mEmerald-C1

    Plasmid
    #53975
    Purpose
    Localization: C1 Cloning Vector, Excitation: 487, Emission: 509
    Depositor
    Type
    Empty backbone
    Tags
    mEmerald
    Expression
    Mammalian
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  21. mEmerald-N1

    Plasmid
    #53976
    Purpose
    Localization: N1 Cloning Vector, Excitation: 487, Emission: 509
    Depositor
    Type
    Empty backbone
    Tags
    mEmerald
    Expression
    Mammalian
    Available Since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
  22. pNIC-CH

    Plasmid
    #26117
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Type
    Empty backbone
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Sept. 17, 2010
    Availability
    Academic Institutions and Nonprofits only
  23. pTpPBR11_accNAT

    Plasmid
    #154034
    Purpose
    Diatom episome backbone for T. pseudonana or C. crytpica
    Depositor
    Type
    Empty backbone
    Use
    Diatom cloning
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
  24. pVenus-N1 (804)

    Plasmid
    #61854
    Purpose
    empty backbone for tagging proteins at the C-terminus with Venus
    Depositor
    Type
    Empty backbone
    Tags
    Venus
    Expression
    Mammalian
    Available Since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
  25. pSwitch-MCS-AttP-2Lox

    Plasmid
    #221505
    Purpose
    Backbone with MCS flanked by attP and Lox sites
    Depositor
    Type
    Empty backbone
    Use
    Cre/Lox
    Available Since
    July 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  26. pBluescript-PB

    Plasmid
    #133885
    Purpose
    piggyBac ITR's in high copy number pBluescript backbone plasmid
    Depositor
    Type
    Empty backbone
    Use
    Piggybac
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 1401 - 1450 of 18154 results