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Showing: 561 - 580 of 48318 results

  2. mOrange2-PCNA-19-SV40NLS-4

    Plasmid
    #57971
    Purpose
    Localization: Nucleus, Excitation: 549, Emission: 565
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Use
    Tags
    mOrange2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 24, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pTetOne-orangutan cGASdeltaN-HA

    Plasmid
    #186898
    Purpose
    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Pongo abelii, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186898-plasmid-map-sequence-id-366562

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  5. pTRE3G-orangutan cGASdeltaN-HA

    Plasmid
    #186872
    Purpose
    Doxycycline-dependent expression of orangutan cGAS gene in mammalian cells by retroviral transduction
    Depositor
    Jonathan Kagan
    Article
    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.
    Insert
    Orangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Pongo abelii, Synthetic)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mutation
    N-terminal truncation
    Promoter
    TRE3G
    Available since
    Oct. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    186872-plasmid-map-sequence-id-366079

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  7. pETSUMO2_Vitiosangium-bGSDM-thrombin

    Plasmid
    #222099
    Purpose
    For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.
    Depositor
    Philip Kranzusch
    Article
    Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:
    Insert
    Vitiosangium-bGSDM-thrombin ORF
    Use
    Tags
    6xHis-SUMO2
    Expression
    Bacterial
    Mutation
    N-terminal methionine deleted and replaced by sin…
    Promoter
    T7
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222099-plasmid-map-sequence-id-437094

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  12. pORANGE_Tubb3 GFP(Y39TAG) KI

    Plasmid
    #182678
    Purpose
    Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Mouse, Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182678-plasmid-map-sequence-id-363084

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  13. pORANGE_NFL linker-3xFLAG KI

    Plasmid
    #182679
    Purpose
    Expression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Nefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182679-plasmid-map-sequence-id-363017

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  16. FKBP-EGFP-RanGAP*

    Plasmid
    #175238
    Purpose
    Bacterial expression and purification, low affinity SUMOylation substrate that can recruited to FRB with rapamycin, point mutation F562A reduces affinity for E2, increasing the Km
    Depositor
    Michael Rosen
    Article
    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d
    Insert
    RANGAP1 (RANGAP1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Amino acid 562 F to A
    Promoter
    tac
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    175238-plasmid-map-sequence-id-345469

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  19. pAS2-his-24::mOrange

    Plasmid
    #124326
    Purpose
    Expressing Nuclear Localized mOrange
    Depositor
    Andrew Leifer
    Insert
    mOrange (his-24 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    124326-plasmid-map-sequence-id-241714

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  20. pAS1-his-24::mOrange

    Plasmid
    #124325
    Purpose
    Expressing Nuclear Localized mOrange
    Depositor
    Andrew Leifer
    Insert
    mOrange (his-24 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    124325-plasmid-map-sequence-id-241712

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Showing: 561 - 580 of 48318 results