178,736 results
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Plasmid#102363PurposeOverexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
TagsNEExpressionMammalianPromoterCMVAvailable sinceDec. 19, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHis-hTG2
Plasmid#100719PurposeExpression of human transglutaminase 2 in E.coliDepositorInsertTGM2 (TGM2 Human)
TagsHisExpressionBacterialMutationsilent mutation Arg580 (AGG--CGT) to enhance expr…PromoterT7Available sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-ZFN-AAVS1
Plasmid#89707PurposeMammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locusDepositorInsertZFN-AAVS1
ExpressionMammalianPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHyg-AID*-6HA
Plasmid#99520PurposeC-terminal AID*-6HA degron cassette with HygR marker for S. cerevisiaeDepositorAvailable sinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AID-BE3
Plasmid#100803PurposeExpresses AID-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertactivation induced cytidine deaminase (AICDA Human, S. pyogenes)
ExpressionMammalianPromoterCMVAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-mRuby2
Plasmid#99123PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoterDepositorInsertmRuby2
UseAAVExpressionMammalianPromoterCAGAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDIRECT_22A
Plasmid#91133PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRExpressionPlantAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSFV3-lacZ
Plasmid#92074PurposeThis is an expression plasmid for production of recombinant genomic SFV RNA encoding beta-galactosidase.DepositorInsertlacZ
UseLacz expression plasmid, for run-off transcriptio…Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CD8a
Plasmid#86050PurposeA mammalian expression plasmid encoding CD8a without tag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD8a (CD8A Human)
ExpressionMammalianPromoterCMV, T7Available sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABEpuro mTurquoise2 LC3B
Plasmid#78518PurposeFluorescent reporter for LC3B visualizationDepositorInsertLC3B (MAP1LC3B Human)
UseRetroviralTagsmTurquoise2ExpressionMammalianPromoterretroviralAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBiex-1 GFPNanobody_SNAP
Plasmid#82711PurposeExpresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.DepositorInsertGFP Nanobody
Tags6xHis Purification tag, FLAG purification tag, an…ExpressionBacterial and InsectPromoterT7 PromoterAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRRL_SRSF2_P95H_mCherry
Plasmid#84023Purposeexpression of SRSF2 mutant in mammalian cellsDepositorInsertSRSF2 (SRSF2 Human)
UseLentiviralTags2TA-mCherry and FlagExpressionMammalianMutationP95HPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKLV2-U6gRNA5(Empty)-PGKmCherry2ABFP-W
Plasmid#67985PurposeCas9 activity reporter (control) with mCherry and BFPDepositorInsertU6gRNA cassette, PGKmCherryABFP cassette, WPRE
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMT-Hrp48-ADARcd-V5
Plasmid#81172Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertHrp48-ADARcd fusion (Hrb27C Fly)
ExpressionInsectPromoterDrosophila metallothionein promoterAvailable sinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FUGW-eGFP-ZEB1 3'UTR
Plasmid#81018PurposeFUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscriptDepositorPublicationInsertZeb1 3'UTR (UTS2R Human)
UseLentiviralAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER13 miR-200c-141
Plasmid#81020PurposeLentiviral vector containing doxycycline inducible human miR-200c. Puromycin selectable.DepositorPublicationInsertmiR-200c (MIR200C Human)
UseLentiviralAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKA-141
Plasmid#79832PurposeExpresses RpBphP1-mCherryDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBphP1-mCherry
ExpressionMammalianPromoterCMVd1Available sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLgw EcoDam-V5-RFC1
Plasmid#59209PurposeMammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloningDepositorTypeEmpty backboneUseLentiviral; DamidTagsDam and V5ExpressionMammalianPromoterHeat Shock Minimal PromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Rab9a
Plasmid#78592PurposeExpress mCherry-Rab9a in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRab9a (RAB9A Human)
TagsmCherry2ExpressionMammalianPromoterCMVAvailable sinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pR26 GFP Dest
Plasmid#74283PurposeGene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloningDepositorInsertRosa26 5-homology region
Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKHR5
Plasmid#74593Purposean alpha-crystallin::Venus (green lens) reporter cassette flanked by FRT sites and with a single loxP site at the 3’ end was inserted in the middle of pKHR4 mcs. Genbank accession number is KU144823.DepositorInsertFRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
UseCre/Lox; Zebrafish expressionPromoteralpha-crystallinAvailable sinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBS-Int
Plasmid#50000PurposeExpresses the integrase on a suicide plasmidDepositorInsertIntegrase from pMV361
ExpressionBacterialPromoterownAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N1_SEPT2
Plasmid#71547Purposemammalian expression of Sept 2 with a GFP fusionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSeptin 2 (Septin2 Mouse)
TagseGFPExpressionMammalianPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-FRT/T0-GFP-PALB2
Plasmid#71113Purposemammalian expession of GFP-PALB2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPALB2 (PALB2 Human)
TagsGFPExpressionMammalianPromoterCMV/TOAvailable sinceDec. 9, 2015AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
ADORA3-Tango
Plasmid#66212PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertADORA3 (ADORA3 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)
Plasmid#64217PurposeExpression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2ADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
hU6 promoter; BbsI sites for sgRNA
H1 promoter; BamHI site for shRNA
UseCRISPRTags3xFLAG, NLS, and T2A-mCherryExpressionMammalianPromoterCBh, H1, and U6Available sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-V5-SBP-C1-HshnRNPM
Plasmid#64924PurposeExpresses V5- and SBP-tagged hnRNPM in mammalian cellsDepositorAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-ESR1
Plasmid#65211PurposeFull length mammalian expression vector for hESR1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertesr1 (ESR1 Human)
TagsAcGFPExpressionMammalianPromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-CRY2-iSH2
Plasmid#66839PurposeExpression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiSH2
TagsmCherry-CRY2ExpressionMammalianPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn-FLEX-rc[Chronos-GFP]
Plasmid#62722PurposeAAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by