We narrowed to 59,737 results for: Gene

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Cas9-GFP_sg_mAMPKa1

Plasmid

#79004

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Prkaa1 (Prkaa1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pEx1-pEF-H2B-mCherry-T2A-rTetR-EED

Plasmid

#78101

Purpose

Expresses rTetR-EED for gene silencing and the nuclear H2B-mCherry marker in mammalian cells.

Depositor

Michael Elowitz

Article

Bintu et al Science. 2016 Feb 12;351(6274):720-4. do

Insert

H2B-mCherry-T2A-rTetR-EED (EED Human, Synthetic)

Use

Synthetic Biology

Tags

rTetR

Expression

Mammalian

Promoter

pEF alpha

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pENO1-dTom-NATr

Plasmid

#52208

Purpose

Constitutive expression of Candida codon-optimized dTomato with NAT selectable marker

Depositor

Robert Wheeler

Article

Gratacap et al Dis Model Mech. 2013 Sep;6(5):1260-70. d

Insert

dTomato

Expression

Bacterial and Yeast

Mutation

codon optimized for Candida species

Promoter

ENO1

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
ACE2(-252)-luc

Plasmid

#31111

Purpose

Luciferase reporter construct for human ACE2 promoter

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

ACE2 promoter (ACE2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -252 to +103

Available Since

Oct. 14, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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p255 pPr-CREM/Ins/CMV-STOP-luc

Plasmid

#8408

Purpose

Modified cre expressed from probasin, separated from the Cre-dependent CMV-STOP-luc cassette by insulators.

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM/insulators/lox/luciferase

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre

Promoter

Probasin, CMV

Available Since

April 26, 2006

Availability

Academic Institutions and Nonprofits only
Stat1 beta pRc/CMV

Plasmid

#8703

Depositor

Jim Darnell

Article

Schindler et al Proc Natl Acad Sci U S A 1992 Aug 15;89(

Insert

STAT1 beta (STAT1 Human)

Expression

Mammalian

Available Since

July 20, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CD81-(TEV)-Cre

Plasmid

#167939

Purpose

human CD81-TEV cleavage site-Cre

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

human CD81 (CD81 Human)

Tags

Cre recombinase and TEV protease cleavage site

Expression

Mammalian

Promoter

CMV

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pHACK-QF2

Plasmid

#80274

Purpose

QF2 HACK backbone. Contains QF2-hsp70, but no gRNAs

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Expression

Insect

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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HA-AMOT p130 deltaEYLI

Plasmid

#32826

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

Insert

AMOT p130 (AMOT Human)

Tags

2xHA

Expression

Mammalian

Mutation

E5D, deletion of last 4 amino acids (EYLI)

Promoter

CMV

Available Since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pGli3a

Plasmid

#25733

Depositor

C.C. Hui

Article

Hui et al Nat Genet. 1993 Mar . 3(3):241-6.

Insert

Gli3 (GLI3 Mouse)

Expression

Bacterial

Available Since

June 24, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pGC95

Plasmid

#19611

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Phsp-16.41::FLP::let-858(3’)

Expression

Worm

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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pGC146

Plasmid

#19613

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Phsp-16.2::FLP::let-858(3’)-(Cbr)unc-119(+)

Use

Flp/frt

Expression

Worm

Promoter

hsp-16.2

Available Since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

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BAG (CC#23)

Plasmid

#15148

Depositor

Connie Cepko

Article

Price et al Proc Natl Acad Sci U S A. 1987 Jan . 84(

Insert

beta-galactosidase

Use

Retroviral; Avian expression

Expression

Mammalian

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only
pLminP_Luc2P_RE45

Plasmid

#90383

Purpose

EGR1 - Early growth response factor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

EGR1

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-hCYP26A1P-E4-mCherry

Plasmid

#135478

Purpose

Human short form promoter of human gene in pGL3-Basic-mCherry plasmid vector (Namely, E4.2)

Depositor

Catharine Ross

Article

Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

Insert

mCherry Open reading Frame

Use

Unspecified

Promoter

Short from promoter of human CYP26A1 (312 bp)

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTF101.2

Plasmid

#134771

Purpose

A binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Article

Testroet et al In Vitro Cell.Dev.Biol.-Plant (2017) 53:

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKAd5f11pES-PmeI

Plasmid

#122698

Purpose

To generate an E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and the exogenous gene expression cassette.

Depositor

Zhuo-Zhuang Lu

Article

Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

Type

Empty backbone

Use

Adenoviral

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z11

Plasmid

#120054

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet-i

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mScarlet-i

Use

Pcr template

Tags

mScarlet-i

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P25

Plasmid

#120036

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-26

Plasmid

#120005

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mAID-Clover

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mAID-Clover

Use

Pcr template

Tags

mAID-Clover

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pWJ1

Plasmid

#90484

Purpose

For gene disruption in Clostridium acetobutylicum ATCC 824 and Clostridium beijerinckii NCIMB8052

Depositor

Sheng Yang

Article

Zhang et al J Microbiol Methods. 2015 Jan;108:49-60.

Insert

pIM13 Origin

Expression

Bacterial

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pBW103ParsR-Amp30C

Plasmid

#78638

Purpose

pBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-t

Depositor

Baojun Wang

Article

Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

Insert

30hrpR-30hrpS-t-hrpL-30gfp-t

Use

Synthetic Biology

Promoter

see insert

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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Pr-deGFP-MGapt

Plasmid

#67734

Purpose

OR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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XLone-Puro NFIL3 P2A eGFP NLS

Plasmid

#140026

Purpose

Tunable and temporal expression control of NFIL3 and nuclear eGFP

Depositor

Xiaoping Bao

Insert

NFIL3 and enhanced Green Fluorescent Protein (NFIL3 Human, Synthetic)

Use

Synthetic Biology

Tags

eGFP

Expression

Mammalian

Promoter

TRE3G

Available Since

May 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJK647

Plasmid

#71701

Purpose

Produces E. coli thioredoxin reductase (EcTrxB)

Depositor

T. J. Kappock

Insert

thioredoxin reductase

Expression

Bacterial

Promoter

T7

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_D4910

Plasmid

#127220

Purpose

Module D with ZmUbi1::BBM

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

BBM

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-URH49 WT

Plasmid

#110127

Purpose

Expresses Flag-tagged human URH49 (DDX39A)

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

URH49 (DDX39A Human)

Tags

Flag

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

April 6, 2019

Availability

Academic Institutions and Nonprofits only

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pJK_proK1_eGFP

Plasmid

#59853

Purpose

Constitutively expresses eGFP with proK1 promoter

Depositor

Timothy Whitehead

Article

Bienick et al PLoS One. 2014 Oct 6;9(10):e109105. doi:

Insert

Enhanced Green Fluorescent Protein

Tags

6x-His

Expression

Bacterial

Mutation

S2R,S65G,S72A

Promoter

proK1

Available Since

Oct. 20, 2014

Availability

Academic Institutions and Nonprofits only

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M-tdTom-ST1

Plasmid

#48678

Purpose

Mammalian tdTomato activation reporter for ST1 with GTCCCCTCCACCCCACAGTG protospacer

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, ST1-PAM, and tdTomato reporter. Compatible with S. thermophilus #1 Cas9

Use

CRISPR

Promoter

Sp6

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only
pBbA5k-RFP

Plasmid

#35282

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

lacUV5

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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