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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,645 results for: sequence

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Showing: 8791 - 8820 of 13645 results

  8. pCEP4-BaLgp140-TM

    Plasmid
    #111843
    Purpose
    Mammalian expression plasmid for extracellular gp140 from the BaL HIV-1 strain fused to a transmembrane tether
    Depositor
    Erik Procko
    Article
    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.
    Insert
    HIV-1 (BaL) Env
    Tags
    CD5 leader sequence and TM helix from MHC class I
    Expression
    Mammalian
    Mutation
    Codon-optimized synthetic gene
    Promoter
    CMV
    Available Since
    March 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    111843-plasmid-map-sequence-id-210251

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  9. pQE80L-SH3

    Plasmid
    #160331
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.
    Depositor
    Sang Taek Jung
    Article
    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12
    Insert
    SH3
    Tags
    6xHis tag
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160331-plasmid-map-sequence-id-326603

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  12. MSCV-myrAkt

    Plasmid
    #49267
    Purpose
    retroviral expression of Bovine Akt with the NH2-terminal myristylation-palmitylation signal sequence
    Depositor
    Lawrence Kane, Arthur Weiss
    Article
    Kane et al Nat Immunol. 2001 Jan;2(1):37-44.
    Insert
    Akt (AKT1 Bovine)
    Use
    Retroviral
    Tags
    IRES-EGFP and myr
    Expression
    Mammalian
    Mutation
    N2D, D149E and A478G compared to AAW71957.1
    Available Since
    Jan. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. mEos3.2GPI

    Plasmid
    #203759
    Purpose
    Encodes the fluorescent protein mEos3.2 followed by the C-terminal sequence derived from CD58 encoding a GPI-attachment signal. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    CD58 c-term (CD58 Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    203759-plasmid-map-sequence-id-404788

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  16. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

    Plasmid
    #169548
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Expression
    Mammalian
    Promoter
    PPGK
    Available Since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169548-plasmid-map-sequence-id-364524

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  19. pAAV-4mtD3cpv

    Plasmid
    #181874
    Purpose
    Expresses the FRET-based mitochondrial calcium indicator 4mtD3cpv under control of the CaMKIIa promoter
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Insert
    4mtD3cpv
    Use
    AAV and Adenoviral
    Expression
    Mammalian
    Mutation
    the 4mtD3cpv CDS differs from the recorded GeneBa…
    Promoter
    CaMKIIa
    Available Since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    181874-plasmid-map-sequence-id-355965

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  22. pcDNA3-DrFLINC-AKAR-Kras

    Plasmid
    #182334
    Purpose
    Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DrFLINC-AKAR
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Available Since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    182334-plasmid-map-sequence-id-363289

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  28. pAAV Syn ChR E90R-D156N-T159C 2A tDimer

    Plasmid
    #52494
    Purpose
    Historical plasmid. For inhibition of neuronal activity, note that Addgene Plasmid #66709 is a light-gated chloride channel with improved anion selectivity (iChloC)
    Depositor
    Thomas Oertner
    Article
    Wietek et al Science. 2014 Mar 27.
    Inserts
    Channelrhodopsin-2
    red fluorescent protein
    Use
    AAV
    Expression
    Mammalian
    Mutation
    E90R, D156N, T159C
    Promoter
    human synapsin-1 and ribosomal skip sequence
    Available Since
    April 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    52494-plasmid-map-sequence-id-81429

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Showing: 8791 - 8820 of 13645 results