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174,739 results

Showing: 11761 - 11800 of 174739 results

  1. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Sarel Fleishman
    Article
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83917-plasmid-map-sequence-id-174928

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  9. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

    Plasmid
    #85165
    Purpose
    Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
    Tags
    AmilCP and RTX
    Expression
    Bacterial
    Promoter
    J23100 promoter
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    85165-plasmid-map-sequence-id-354765

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  16. pZA16mflon

    Plasmid
    #75439
    Purpose
    Plasmid for arabinose-induced expression of mf-lon. Note that this plasmid contains the original mf-lon cassette that is not codon optimized for expression in E. coli.
    Depositor
    James Collins
    Article
    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.
    Insert
    mf-lon
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75439-plasmid-map-sequence-id-147407

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  21. pMAL-c4X

    Plasmid
    #75288
    Purpose
    Expresses proteins in the cytoplasm as fusions to a high-binding mutant (A313V) maltose-binding protein
    Depositor
    Paul Riggs
    Article
    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1
    Type
    Empty backbone
    Tags
    maltose-binding protein
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 19, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    75288-plasmid-map-sequence-id-142417

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  24. pTiGc

    Plasmid
    #78314
    Purpose
    Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).
    Depositor
    Samantha Sampson
    Article
    Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/
    Inserts
    TurboFP635
    GFP
    Use
    Synthetic Biology; Mycobacterial replicating vect…
    Expression
    Bacterial
    Promoter
    hsp60 and hsp60(ribo)
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  26. pEMS2115

    Plasmid
    #49140
    Purpose
    The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.
    Depositor
    Elizabeth Simpson
    Article
    Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414
    Insert
    ssAAV-Ple155-emGFP-WPRE
    Use
    AAV
    Expression
    Mammalian
    Promoter
    MiniPromoter Ple155 from the human PCP2 gene
    Available Since
    May 10, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    49140-plasmid-map-sequence-id-245687

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  31. pQCXIP-F-luc

    Plasmid
    #73058
    Purpose
    Retroviral expression of firefly luciferase for in vivo imaging
    Depositor
    Michael Grusch
    Insert
    Firefly luciferase
    Use
    Retroviral
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73058-plasmid-map-sequence-id-134128

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  32. DH-BB(5'SIN;TE12ORF)

    Plasmid
    #72309
    Purpose
    Defective Helper to produce neurotropic, propagation-incompetent Sindbis virus
    Depositor
    Anthony Zador
    Insert
    DH-BB(5'SIN;TE12ORF)
    Use
    Sindbis virus helper construct
    Available Since
    Jan. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    72309-plasmid-map-sequence-id-145921

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  33. pcDNA3.1+ eiF4A2 myc HIS

    Plasmid
    #71658
    Purpose
    mammalian expression of eiF4A2
    Depositor
    Gideon Dreyfuss
    Insert
    EIF4A2 (EIF4A2 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  34. pCDNA_Lifeact-GFP_NLS-mCherry

    Plasmid
    #69058
    Purpose
    Dual Fluorescent Reporter for F-actin and Nuclei
    Depositor
    Olivier Pertz
    Insert
    LifeAct-GFP_IRES_NLS-mCherry
    Tags
    Lifeact-GFP, NLS-mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69058-plasmid-map-sequence-id-123543

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  40. CHRM1-Tango

    Plasmid
    #66248
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    CHRM1 (CHRM1 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 11761 - 11800 of 174739 results