164,223 results

1. pAAV-EF1a-FLEX-GTB

   Plasmid

   #26197

   Depositor

   Edward Callaway

   Article

   Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

   Insert

   Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   Expression

   Mutation

   TVA, rabies glycoprotein are mammalian codon opti…

   Promoter

   Available Since

   Nov. 11, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIB4

   Plasmid

   #25453

   Depositor

   Benjamin Glick

   Article

   Sears et al Yeast. 1998 Jun 15;14(8):783-90.

   Insert

   Pichia pastoris HIS4 (PAS_chr1-4_0160 Pichia pastoria)
   

   Use

   Pichia pastoris expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GPR15-Tango

   Plasmid

   #66325

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR15 (GPR15 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pY108 (lenti-AsCpf1)

   Plasmid

   #84739

   Purpose

   Lenti virus delivery of AsCpf1 and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huAsCpf1
   puromycin resistance gene
   

   Use

   Lentiviral

   Tags

   3xHA, NLS, and P2A

   Expression

   Mutation

   Promoter

   EFS

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-Int

   Plasmid

   #50000

   Purpose

   Expresses the integrase on a suicide plasmid

   Depositor

   Peter Sander, Brian Robertson

   Article

   Springer et al Int J Med Microbiol. 2001 Mar;290(8):669

   Insert

   Integrase from pMV361
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   own

   Available Since

   April 20, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pICH41780

   Plasmid

   #48019

   Depositor

   Sylvestre Marillonnet

   Article

   Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 3, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pEG302 22aa SunTag NtDRMcd nog

   Plasmid

   #115488

   Purpose

   CRISPR-Cas9 SunTag system to target NtDRMcd to specific loci of interest (nog=no guide)

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   NOS_NLS_GB1_NLS_linker_DRMcd_linker sfGFP_scFv_UBQ10_Insulator_ UBQ10_Ω dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   March 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV_PACLight1

   Plasmid

   #197864

   Purpose

   Mammalian expression of PACLight1 biosensor for PACAP (Pituitary Adenylate Cyclase Activating Peptide)

   Depositor

   Tommaso Patriarchi

   Article

   Cola et al Elife. 2024 Aug 15;13:RP96496. doi: 10.7

   Insert

   PACLight1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHDM-Tat1b

   Plasmid

   #164442

   Purpose

   Encodes HIV-1 Tat accessory protein for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

   Insert

   HIV-1 Tat
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLSLR

    Plasmid

    #51500

    Purpose

    Single component plant T-DNA plasmid. Contains cis- and trans-acting replicational elements from the bean yellow dwarf virus in an LIR-SIR-Rep/RepA-LIR orientation.

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Type

    Empty backbone

    Use

    Plant t-dna plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDB179-DepoPETase

    Plasmid

    #218702

    Purpose

    Encodes codon optimized DepoPETase enzyme for bacterial expression

    Depositor

    Gregg Beckham

    Article

    Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

    Insert

    DepoPETase
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG1243 NES-ECFP-FIREmate

    Plasmid

    #205169

    Purpose

    Expresses NES-ECFP-FIREmate in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Insert

    NES-ECFP-FIREmate
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-Lrrc59

    Plasmid

    #226565

    Purpose

    Encodes Lrrc59 protein laebeled with mCherry

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    Lrrc59 (Leucine-rich repeat containing protein 59) (LRRC59 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB80-hKif5b(1-560)rigor-2xmNeongreen

    Plasmid

    #174649

    Purpose

    Live-cell marker for stable microtubules

    Depositor

    Lukas Kapitein

    Article

    Jansen et al J Cell Biol. 2023 May 1;222(5):e20210610

    Insert

    hKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
    

    Use

    Tags

    mNeongreen-mNeongreen

    Expression

    Mammalian

    Mutation

    rigor mutation G234A

    Promoter

    Chicken beta-actin

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. His-Tev-UbE1

    Plasmid

    #186804

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin activating enzyme His-Tev-UbE1.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-UbE1 (UBA1 Human)
    

    Use

    Tags

    His6-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI-EGFP-NR1 wt

    Plasmid

    #45446

    Depositor

    Robert Malinow, Andres Barria

    Article

    Barria et al Neuron. 2002 Jul 18;35(2):345-53.

    Insert

    NR1 (Grin1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    EGFP inserted after the predicted signal peptide …

    Promoter

    CMV

    Available Since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKO403_lacZ_Cm

    Plasmid

    #174725

    Purpose

    Bifidobacterium-E.coli shuttle vector for seamless gene cloning

    Depositor

    Tohru Suzuki

    Article

    Altaib et al Microbiol Resour Announc. 2022 Jan 20;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBabe puro c-met WT

    Plasmid

    #17493

    Depositor

    Joan Brugge

    Article

    Wrobel et al J Cell Biol. 2004 Apr 26. 165(2):263-73.

    Insert

    c-Met (Met Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCX-SpiCee

    Plasmid

    #140836

    Purpose

    mammalian expression of SpiCee, a buffer of calcium that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.CMV.LacZ.bGH

    Plasmid

    #105531

    Purpose

    AAV expression of LacZ from CMV promoter

    Depositor

    James M. Wilson

    Has Service

    AAV5 and AAV8

    Insert

    LacZ
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pLV-tTRKRAB-red

    Plasmid

    #12250

    Depositor

    Didier Trono

    Article

    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

    Insert

    TetR-KRAB fusion and dsRED
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    dsRed added to backbone.

    Promoter

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pN7-S15

    Plasmid

    #190665

    Purpose

    Expresses N7 ribosomal protein S15

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    human codon optimized N7 S15
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-cCFP

    Plasmid

    #185361

    Purpose

    For mammalian expression of C-term CFP for split-CFP

    Depositor

    Ayaz Najafov

    Insert

    CFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    June 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Slug_pGL2

    Plasmid

    #31695

    Depositor

    Paul Wade

    Article

    Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

    Insert

    Slug (SNAI2 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pIA33

    Plasmid

    #120803

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

    Depositor

    David S. Weiss, Craig Ellermeier

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Inserts

    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Base-pairing region targets red fluorescent prote…

    Promoter

    Pgdh and Pxyl

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGH044_mCherry

    Plasmid

    #85412

    Purpose

    lentiviral expression vector for mCherry

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP-PTEN

    Plasmid

    #13039

    Depositor

    Alonzo Ross

    Article

    Liu et al J Cell Biochem. 2005 Oct 1. 96(2):221-34

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pmCherry-Synaptojanin-1-145

    Plasmid

    #223563

    Purpose

    Expresses the short isoform of SYNJ1 (neuronal) with mCherry tagged at the n-terminus

    Depositor

    Pietro De Camilli

    Article

    Mohd Rafiq et al Proc Natl Acad Sci U S A. 2024 Apr 23;12

    Insert

    SYNJ1 (SYNJ1 Human)
    

    Use

    Tags

    His and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEB1-moxCerulean3

    Plasmid

    #103975

    Purpose

    Plasmid encoding moxCerulean3

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    moxCerulean3
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F64L, Y66W, …

    Promoter

    proC

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 3xERRE/ERE-luciferase

    Plasmid

    #37852

    Depositor

    Rebecca Riggins

    Article

    Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

    Insert

    3 copies of ERRE/ERE upstream of luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available Since

    Aug. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pSEVA6213S

    Plasmid

    #122094

    Purpose

    constitutive expression of I-SceI for genome engineering in Pseudomonas

    Depositor

    Pablo Ivan Nikel

    Article

    Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

    Insert

    I-SceI restriction endonuclease
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Pem7

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Integrin Beta1-mPA-GFP

    Plasmid

    #186302

    Purpose

    Expresses mPA-GFP tagged Integrin beta-1 in mammalian cells on a CMV promoter

    Depositor

    Pakorn Kanchanawong

    Article

    Jain et al Nat Protoc. 2022 Jul 27. pii: 10.1038/s4

    Insert

    Integrin Beta 1 (ITGB1 Human)
    

    Use

    Tags

    mPA-GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3/HA-SUMO3 (Sentrin 2)

    Plasmid

    #17361

    Purpose

    Mammalian expression of human SUMO3 with HA tag

    Depositor

    Edward Yeh

    Article

    Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

    Insert

    SUMO3 (SUMO3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBa.TfR.GFP

    Plasmid

    #45060

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Burack et al Neuron. 2000 May;26(2):465-72.

    Insert

    TfR (TFRC Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta Actin

    Available Since

    Oct. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSBbi_RP_lbMicroID2-HA

    Plasmid

    #188522

    Purpose

    Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    lbMicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pcDNA3-Cyto-ExRai-AktAR2

    Plasmid

    #184048

    Purpose

    Cytosol-targeted ExRai-AktAR2.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    Cyto-ExRai-AktAR2
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PLVX_SPTA1_IGF2BP1_IRES_PURO

    Plasmid

    #100016

    Purpose

    To over-express IGF2BP1 in Adult Erythroblasts using tissue specific promoter with IRES for puromycin selection.

    Depositor

    Jeffery Miller

    Article

    de Vasconcellos et al Proc Natl Acad Sci U S A. 2017 Jul 11;11

    Insert

    Insulin like growth factor 2 mRNA binding protein 1 (IGF2BP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SPTA1 erythroid specific promoter

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEGFP-RhoA Biosensor

    Plasmid

    #68026

    Purpose

    C-terminus of Anillin in mammalian expression vector pEGFP-C1

    Depositor

    Michael Glotzer

    Article

    Piekny et al Curr Biol. 2008 Jan 8;18(1):30-6. Epub 2

    Insert

    Anillin (AHD+PH) (ANLN Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. shYAP1 # 2

    Plasmid

    #42541

    Depositor

    William Hahn

    Article

    Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV.CAG.Flex.NES-jRGECO1a.WPRE.SV40

    Plasmid

    #100852

    Purpose

    AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the CAG promoter

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

    Has Service

    AAV1

    Insert

    jRGECO1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    June 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge