We narrowed to 6,132 results for: pCas

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JG693: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X3)

Plasmid

#104570

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to three DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1(D832A)-DmrA(x3)

Tags

3x FLAG, 3x HA, and NLS

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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MST1R gRNA (BRDN0001487060)

Plasmid

#78050

Purpose

3rd generation lentiviral gRNA plasmid targeting human MST1R

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MST1R (MST1R Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MST1R gRNA (BRDN0001145791)

Plasmid

#78051

Purpose

3rd generation lentiviral gRNA plasmid targeting human MST1R

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MST1R (MST1R Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MST1R gRNA (BRDN0001145169)

Plasmid

#78052

Purpose

3rd generation lentiviral gRNA plasmid targeting human MST1R

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MST1R (MST1R Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-SpG-P2A-EGFP (RTW4177)

Plasmid

#139988

Purpose

CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Walton et al Science. 2020 Apr 17;368(6488):290-296.

Insert

human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

Promoter

CMV and T7

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-bpNLS-eeBxb1 (CJT555)

Plasmid

#248209

Purpose

eeBxb1 recombinase with N-terminal bpNLS, expressed from a CMV promoter.

Depositor

Benjamin Kleinstiver

Article

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

bpNLS-eeBxb1

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

eeBxb1(V74A, E229K, V375I)

Promoter

CMV and T7

Available Since

April 15, 2026

Availability

Academic Institutions and Nonprofits only

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pT7-PE7 for IVT

Plasmid

#214813

Purpose

Template for in vitro transcription of PE7

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Promoter

T7 (inactivated)

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PDi-CRISPRn

Plasmid

#73500

Purpose

Dox-inducible CRISPR nuclease (CRISPRn) knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

Cas9
rtTA

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pHLS-EF1a-FKBP12-Gag(HIV)

Plasmid

#138476

Purpose

Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

HIV Gag

Tags

FRBP12 and Lynn Myristolation site

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9Pubi-H

Plasmid

#66187

Purpose

Expression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

maize ubiquitin promoter (Pubi)

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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PB-PEmax

Plasmid

#187893

Purpose

All-in-one prime editor piggyBac transposon with PEmax

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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PB-PEmax SpG

Plasmid

#187895

Purpose

All-in-one prime editor piggyBac transposon with PEmax, SpG variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_KK_SpG-MMLV-RT_co-P2A-PAC_dTK

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

BPSV40 NLS and c-Myc NLS

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

Plasmid

#107270

Purpose

Expresses Cas9 upon induction with doxicycline.

Depositor

Pablo Menéndez

Article

Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

Inserts

hSpCas9
rtTA
bGH polyA-SV40polyA

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG and TRE

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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CGBE1-NG (pBM1246)

Plasmid

#140256

Purpose

CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

eUNG-BE4max(R33A)_NG-ΔUGI

Expression

Mammalian

Mutation

R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

Promoter

CMV

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pYLCRISPR/Cas9Pubi-B

Plasmid

#66188

Purpose

expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection

Depositor

Yao-Guang Liu

Article

Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

Insert

Cas9

Expression

Plant

Mutation

plant-codon optimized with higher GC contents (62…

Promoter

maize ubiquitin promoter (Pubi)

Available Since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pEX-PK-hLC3∆G

Plasmid

#61459

Purpose

Express pHluorin-mKate2-hLC3∆G (PK-hLC3∆G), a negative control for pHluorin-mKate2-hLC3 (PK-hLC3), in mammalian cells for monitoring autophagy

Depositor

Isei Tanida

Article

Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi

Insert

PK-hLC3∆G (MAP1LC3B Human)

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pC-G418-YR

Plasmid

#61767

Purpose

Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing nptII (neomycin phosphotransferase II) selectable marker on transfer DNA (TDNA).

Depositor

Ken Haynes

Article

Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

Inserts

nptII gene
2 micron origin or replication and URA3 gene for S. cerevisiae

Use

Ternary vector for agrobacterium mediated transfo…

Promoter

trpC promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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PX458-sgTSC2

Plasmid

#128098

Purpose

CRISPR gRNA against human TSC2 with Cas9 from S. pyogenes and 2A-EGFP

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

sgRNA against human TSC2 (TSC2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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Ai65(RCFL-tdT) targeting vector

Plasmid

#61577

Purpose

Target a Flp and Cre-dependent tdTomato expression cassette to the mouse Rosa26 locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

pCAG-FSF-LSL-tdTomato

Use

Cre/Lox and Mouse Targeting

Promoter

CAG

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSA091 PSMD14_IVS2-Cas9-BFP

Plasmid

#249141

Purpose

Overexpression of SpCas9-BFP with PSMD14 IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

PSMD14_IVS2-Cas9-TagBFP (PSMD14 S. pyogenes Cas9, synthetic, Human)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

PSMD14 IVS2 intron

Promoter

EF1a

Available Since

Jan. 8, 2026

Availability

Academic Institutions and Nonprofits only

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