164,223 results

1. pEGFP-Nup88

   Plasmid

   #64283

   Purpose

   GFP-tagged mammalian expression of full-length Nup88

   Depositor

   Birthe Fahrenkrog

   Article

   Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

   Insert

   Nup88 (NUP88 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCDH-EF1-DIO-tdTomato

   Plasmid

   #72254

   Purpose

   Express tdTomato under EF-1 promoter when Cre is expressed by another vector

   Depositor

   Kazuhiro Oka

   Insert

   tdTomato
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   elongation factor-1

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-CAG-FLEX-BFP2-(mCherry)’

   Plasmid

   #115234

   Purpose

   Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

   Depositor

   Ian Wickersham

   Article

   Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

   Insert

   mTagBFP2 and mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNCA

   Plasmid

   #17363

   Depositor

   Stephen Goff

   Article

   Colicelli et al J Mol Biol. 1988 Jan 5. 199(1):47-59.

   Insert

   MMLV proviral DNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 1, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

   Plasmid

   #163059

   Purpose

   mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   mito-roGFP2-Orp1
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pN1-PinkyCaMP

   Plasmid

   #232861

   Purpose

   A vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. HCT46_rAAV-eHGT_1188m-minBglobin-SYFP2-WPRE3-BGHpA

   Plasmid

   #223943

   Purpose

   Direct-expressing SYFP2 AAV virus

   Depositor

   Tanya Daigle, Allen Institute for Brain Science

   Article

   Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   minBglobin

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. HCT49_rAAV-3Xcore-eHGT_1139m-minBglobin-SYFP2-WPRE3-BGHpA

   Plasmid

   #223944

   Purpose

   Direct-expressing SYFP2 AAV virus

   Depositor

   Tanya Daigle, Allen Institute for Brain Science

   Article

   Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   minBglobin

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2+ H2B-mTagBFP2

   Plasmid

   #99267

   Purpose

   Contains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

   Depositor

   Philipp Keller

   Insert

   mTagBFP2-3XHA
   

   Use

   Tags

   H2B

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available Since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CMV/TO GFP-Zeo DEST (719-1)

    Plasmid

    #17431

    Purpose

    3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41

    Plasmid

    #213024

    Purpose

    Expresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusion

    Depositor

    Martin S. Taylor, Oliver Weichenrieder

    Article

    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

    Insert

    LINE-1 ORF2p (238-1061)
    

    Use

    Tags

    His6-MBP-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7 / Lac

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF3334

    Plasmid

    #144810

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SP100 (SP100 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWZL-Neo-Myr-Flag-DEST

    Plasmid

    #15300

    Purpose

    a Gateway-compatible retroviral destination vector which adds a myristoylation sequence and a FLAG tag to each introduced ORF

    Depositor

    Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. AICSDP-079:DCP1A-mEGFP

    Plasmid

    #193922

    Purpose

    Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A

    Depositor

    Allen Institute for Cell Science

    Insert

    DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
    

    Use

    CRISPR; Donor template

    Tags

    mEGFP-linker

    Expression

    Mutation

    homology arms contain point mutations to disrupt …

    Promoter

    Available Since

    Dec. 22, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. TFORF2596

    Plasmid

    #142338

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RORB (RORB Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PCMV-flag-Caspase11

    Plasmid

    #21145

    Depositor

    Junying Yuan

    Article

    Li et al Nat Cell Biol. 2007 Mar . 9(3):276-86.

    Insert

    Caspase11 (Casp4 Mouse)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiPGK Hygro DEST H2B-mCerulean3

    Plasmid

    #90234

    Purpose

    Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    H2B
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. RMCE halo-flag DNA-PKcs

    Plasmid

    #233918

    Purpose

    Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Use

    Tags

    flag-halo

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. Dat - EGFP - Tol2

    Plasmid

    #85418

    Purpose

    for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene

    Depositor

    Marc Ekker

    Article

    Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:

    Insert

    dopamine transporter (slc6a3 Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    dat

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

20. pGL3 basic E2

    Plasmid

    #48747

    Purpose

    Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    mPer2 promoter/enhancer region containing E-box2 (-112 to +98)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTR01F/hFGFR2c-V5

    Plasmid

    #236013

    Purpose

    for PiggyBac mediated integration and stable expression of hFGFR2c protein

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    human FGFR2c (FGFR2 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    truncated CMV promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-4T1-RhoA G17A

    Plasmid

    #69357

    Purpose

    expression of nucleotide free RhoA in bacterial cells

    Depositor

    Rafael Garcia-Mata

    Article

    Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

    Insert

    RhoA (RHOA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G17A

    Promoter

    tac

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pGIPz-PD-L1 WT

    Plasmid

    #121486

    Purpose

    2nd generation lentiviral vector for over expressing human PD-L1 WT with PD-L1 shRNA

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBFC0984

    Plasmid

    #231992

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with 2xBsaI spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and pTet

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. IFNGR2_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176939

    Purpose

    Mammalian expression vector encoding IFNGR2 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    IFNGR2 (IFNGR2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pORTMAGE-Pa1

    Plasmid

    #138475

    Purpose

    Enables high-efficiency recombineering in P. aeruginosa

    Depositor

    George Church

    Article

    Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

    Inserts

    PapRecT
    mutL E36K
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    E36K

    Promoter

    Available Since

    June 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBabe_puro_DEST_Flag_PLD1

    Plasmid

    #45268

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    PLD1 (PLD1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKCcas9dO

    Plasmid

    #62552

    Purpose

    High efficiency Streptomyces genome editing by CRISPR/Cas9 system

    Depositor

    Weihong Jiang

    Article

    Huang et al Acta Biochim Biophys Sin (Shanghai). 201

    Inserts

    Scocas9
    sgRNA ()
    act-orf4 homology arm
    

    Use

    CRISPR; Bacterial genome editing

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    j23119 and ptipA

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MSCV-N LMP1

    Plasmid

    #37962

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    LMP1 (LMP-1 H. Herpesvirus 4 (EBV))
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    PKG

    Available Since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP(C1)-PP1alpha

    Plasmid

    #44224

    Depositor

    Angus Lamond, Laura Trinkle-Mulcahy

    Article

    Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

    Insert

    PP1A (PPP1CA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAAV-tMCK-ER-TurboID

    Plasmid

    #149412

    Purpose

    Muscle-specific expression of ER-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Article

    Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

    Insert

    ER-TurboID
    

    Use

    AAV

    Tags

    HA and V5

    Expression

    Mutation

    Promoter

    tMCK

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. Lenti_sgRNA(MS2)_MCP-KRAB-IRES-zsGreen1

    Plasmid

    #138460

    Purpose

    lenti sgRNA cloning backbone with MS2 loops and EF1a-MCP-KRAB

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    MCP-KRAB-IRES-zsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available Since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSEM318 - sgRNA mosTI unc-119 single-copy

    Plasmid

    #159822

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI unc-119 single-copy
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGEX-4T1_ATG4B_BamHI_NotI

    Plasmid

    #190862

    Purpose

    Plasmid for the expression and purification of of ATG4B. Internal reference: SMC1392

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    ATG4B (ATG4B Human)
    

    Use

    Tags

    GST, Thrombin Cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAG-NCad-B3

    Plasmid

    #191668

    Purpose

    ddFP-B3-inserted mouse N-cadherin

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Commun Biol. 2022 Oct 7;5(1):1065. doi:

    Insert

    N-cadherin
    

    Use

    Tags

    HA and ddFP-B3

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin promoter

    Available Since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

    Plasmid

    #128386

    Purpose

    Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption

    Depositor

    Craig Duvall

    Article

    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss

    Inserts

    NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Synthetic, Human)
    Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)
    

    Use

    Lentiviral, Luciferase, and Synthetic Biology

    Tags

    C-terminal luc2 fragment and N-terminal luc2 frag…

    Expression

    Mammalian

    Mutation

    N terminal carbohydrate recognition domain fused …

    Promoter

    EF1A and IRES

    Available Since

    Jan. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. DsRed-rab7 DN

    Plasmid

    #12662

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    T22N

    Promoter

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pC1-SypHer3s

    Plasmid

    #108118

    Purpose

    Ratiometric pH probe with enhanced brightness for mammalian cells expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s
    

    Use

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. N370S GBA pcDNA3.1

    Plasmid

    #188583

    Purpose

    Ectopic expression N370S GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N370S

    Promoter

    CMV

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSF4 CMV intron renilla TRICK CTE polyA

    Plasmid

    #84443

    Purpose

    TRICK reporter mRNA

    Depositor

    Jeffrey Chao

    Article

    Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

    Insert

    Renill-TRICK
    

    Use

    Luciferase; Frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-CMV

    Available Since

    Nov. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge