We narrowed to 14,669 results for: flag
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Plasmid#198572PurposeSPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vectorDepositorInsertSPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
UseRetroviralTags3xFlag-GFPExpressionMammalianMutationchanged glutamic acid E164 to lysine (E164K)Available SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO5d.EFS.SpCas9.P2A.BSD
Plasmid#57821PurposeLentiviral Vector for SpCas9 Expression without sgRNA, Blasticidin resistance, EFS Promoter drivenDepositorAvailable SinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40
Plasmid#101064PurposeAAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas ServiceAAV1InsertCaMPARI2_L398T
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGIPz-mPD-L1
Plasmid#121488PurposeExpression of mouse PD-L1 WTDepositorInsertPD-L1
UseLentiviralTagsFlagExpressionMammalianPromoterCMVAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
EF.STAT3DN.Ubc.GFP
Plasmid#24984PurposeDepositorInsertSTAT3 (STAT3 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationY705F--dominant negativeAvailable SinceJune 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMXS-mitoGshF-blasticidin
Plasmid#210314PurposeExpresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cellsDepositorInsertBifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
UseRetroviralTagsFLAG peptide, Homo sapiens ACO2 mitochondrial tar…MutationCodon optimized for expression in human cellsAvailable SinceJan. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-pA-M.EcoGII
Plasmid#192873PurposepA-M.EcoGII expression plasmid, originally created for BIND&MODIFY method, a Long-range single-molecule mapping of chromatin modification in eukaryotes. doi: https://doi.org/10.1101/2021.07.08.451578DepositorInsertpA-M.EcoGII
Tags3X Flag TagExpressionBacterialPromoterT7Available SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1484 - pAAV SYN1 HA-hM4D(Gi)
Plasmid#121538PurposeAn adeno-associated viral vector expressing HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoterDepositorHas ServiceAAV2 and AAV5InsertHA-tagged hM4D(Gi) (CHRM4 Human)
UseAAVTagsHAExpressionMammalianPromoterSYN1Available SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-H396K-WPRE-SV40
Plasmid#101062PurposeAAV vector expressing CaMPARI2_H396K (Kd = 360nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorInsertCaMPARI2-H396K
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE8e-NG
Plasmid#181754PurposeMMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE8e-NG
TagsFlagExpressionMammalianPromoterCMVAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-YAPS94A
Plasmid#84010Purposeexpresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertYAP1 (siRNA insensitive) (YAP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationS94APromoterTRE-CMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE8e
Plasmid#181751PurposeMMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE8e
TagsFlagExpressionMammalianPromoterCMVAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-Cas9
Plasmid#181752PurposeMMLV-Gag fused to 3x NES-SpyCas9 with the TSTLLMENSS cleavage site in between NES and Cas9. For production of virus like particles with Cas9 nuclease RNP cargo.DepositorInsertMMLV-Gag-3xNES-Cas9 (Spy)
TagsFlagExpressionMammalianPromoterCMVAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40
Plasmid#101060PurposeAAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas ServiceAAV1InsertCaMPARI2
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ-DM
Plasmid#64880Purposelentiviral expression of human POLQ double mutant (in POL and HDL domains)DepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationK121M and D2330A,Y2331APromoterEF1alphaAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE3G-FNLS-PGK-Puro
Plasmid#110847PurposeLentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterTRE3GAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)
Plasmid#139997PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-P2A-GFP-PGK-Puro
Plasmid#110869PurposeLentiviral vector for constitutive expression of FNLS-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40
Plasmid#101061PurposeAAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas ServiceAAV1InsertCaMPARI2-F391W
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightG
Plasmid#187179PurposeExpresses green norepinephrine indicator nLightG in neuronal cellsDepositorHas ServiceAAV9InsertnLightG
UseAAVTagsFlag tagPromoterhuman Synapsin-1Available SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
dCas9-CBP FL
Plasmid#179550Purposeencodes S. pyogenes dCas9 with c-terminal fusion of full length human CBP driven by EF-1alpha promoterDepositorInsertS. pyogenes dCas9 with c-terminal fusion of full length human CBP (CREBBP Human)
UseLentiviralTagsFlag TagExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterEF-1alphaAvailable SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
LiOn-CMV∞Cre
Plasmid#154018PurposeVector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposaseDepositorInsertCre-FLAG
ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1_nLightR
Plasmid#187180PurposeExpresses red norepinephrine indicator nLightR in neuronal cellsDepositorHas ServiceAAV9InsertnLightR
UseAAVTagsFlag tagPromoterhuman Synapsin-1Available SinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
GPER-Tango
Plasmid#66299PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)
Plasmid#139996PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRHR1-Tango
Plasmid#66256PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-EBF1
Plasmid#96965PurposeDox-inducible expression of Ebf1 in mammalian cellsDepositorAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-GRM3 WT
Plasmid#31798DepositorAvailable SinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNTI194
Plasmid#84266PurposeExpresses Avi-tagged RPL1 and humanized birADepositorInsertsTagsAvi tag, Flag, Gly/Ser linker, and TEV siteExpressionMammalianMutationR33A, A34E (*see below)PromoterCMV and P2A peptideAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MMLVgag-3xNES-ABE7.10-NG
Plasmid#181753PurposeMMLV-Gag fused to 3xNES-ABE7.10-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE7.10-NG
TagsFlagExpressionMammalianPromoterCMVAvailable SinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40
Plasmid#101063PurposeAAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorInsertCaMPARI2_F391W-G395D
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9.mCherry
Plasmid#208342PurposeLentivirus with EF1a driven Cas9 linked (P2A) to mCherry.DepositorArticleInsertsCas9
mCherry
UseCRISPR and LentiviralTagsnuclear localization sequence (NLS) and FLAG tagPromoterEF-1aAvailable SinceJan. 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+/-FLI1-C‐(K)‐DYK
Plasmid#129497PurposeMammalian expression of human FLI1DepositorAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-P2A-Puro
Plasmid#110841PurposeLentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-PP-human-CHMP3(150)
Plasmid#154181Purposeexpresses human CHMP3(150) in mammalian cellsDepositorInsertCHMP3 (CHMP3 Human)
TagsFLAG and Strep-Tag IIExpressionMammalianMutationC-terminal truncation of CHMP3 at amino acid 150PromoterCAGAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1-P2A-GFP-PGK-Puro
Plasmid#110863PurposeLentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)DepositorInsertCas9-HF1
UseLentiviralTags3X FLAGMutationN497A, R661A, Q695A, Q926APromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
CXCR4-Tango
Plasmid#66262PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
hu-TLR4 cDNA wt pDEST40
Plasmid#42646Purposedestination vector for expression of human TLR4 in mammalian cellsDepositorAvailable SinceMarch 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B EDD
Plasmid#37188DepositorInsertEDD (UBR5 Human)
Tags6x His Tag and FLAGExpressionMammalianMutationPlease see Depositor CommentsPromoterCMVAvailable SinceSept. 18, 2012AvailabilityAcademic Institutions and Nonprofits only