We narrowed to 11,977 results for: pcDNA

1. pCDNA3 5'cMyc-SMCl- S957A/S966A

   Plasmid

   #32372

   Depositor

   Michael Kastan

   Article

   Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

   Insert

   SMC1A (SMC1A Human)
   

   Use

   Tags

   c-Myc

   Expression

   Mammalian

   Mutation

   S957A/S996A

   Promoter

   Available since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1-tvD-Cit-tevs-DHFR

   Plasmid

   #116054

   Purpose

   Reporter plasmid for measuring protease activity

   Depositor

   Michael Elowitz

   Article

   Gao et al Science. 2018 Sep 21;361(6408):1252-1258

   Insert

   tvD-Cit-tevs-DHFR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-myc3-CUL4A delta N52

   Plasmid

   #19952

   Depositor

   Yue Xiong

   Article

   Liu et al Mol Cell. 2002 Dec . 10(6):1511-8.

   Insert

   CUL4A (cullin 4A) (CUL4A Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   Missing 52 residues from N terminal CUL4A.

   Promoter

   Available since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3 Flag-MBD1 R44Q-14-mVenusC

   Plasmid

   #191394

   Purpose

   R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus

   Depositor

   Albert Jeltsch

   Article

   Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

   Insert

   pcDNA3 Flag-MBD1 R44Q-14-mVenusC
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   R44Q

   Promoter

   CMV

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

   Plasmid

   #181853

   Purpose

   mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5 FRT TO Myc ArB WT RNAiR

   Plasmid

   #59806

   Purpose

   Allows the integration of Myc ArB in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora B (AURKB Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   RNAi resistant

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3 Flag p38 gamma (agf)

   Plasmid

   #20354

   Depositor

   Roger Davis

   Article

   Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

   Insert

   pDCNA3 Flag p38 gamma(agf) (Mapk12 Rat)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Replace Thr 183 and Tyr 185 with Ala and Phe resp…

   Promoter

   Available since

   April 29, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA5/FRT/TO SBBP-CDK1 WT (2007)

   Plasmid

   #61847

   Purpose

   mammalian expression of CDK1 WT, used for Ips

   Depositor

   Jonathon Pines

   Insert

   CDK1 (CDK1 Human)
   

   Use

   Tags

   SBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3.1 LIC 6A CARD8-FLAG S297A

   Plasmid

   #169949

   Purpose

   Mammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S297A

   Promoter

   CMV

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Flag mTOR S2035T R2109A

    Plasmid

    #26606

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S2035T R2109A

    Promoter

    Available since

    Nov. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1/CIP2A(1-905) R522D V5 His

    Plasmid

    #119289

    Purpose

    CIP2A R522D V5 overexpression, CIP2A dimer defective mutant

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Use

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    R522D

    Promoter

    T7

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-CD16.7-hPC1-eGFP-3HA

    Plasmid

    #108401

    Purpose

    Express cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchor

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Myc3-Cul3 H2M/H5M

    Plasmid

    #21591

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    cullin 3 (CUL3 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Mutated residues H2M/H5M

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA-Cas9-T2A-STOP-TdT

    Plasmid

    #126425

    Purpose

    Expresses Cas9 but not TdT in mammalian cells; control for pcDNA-Cas9-T2A-TdT. (pTBL552)

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    Cas9-T2A-STOP-TdT (DNTT Streptococcus pyogenes, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alanine-6 of TdT has been changed to a STOP codon

    Promoter

    pCMV

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 SARS-CoV-2 S D614-trunc

    Plasmid

    #158076

    Purpose

    Expresses SARS-CoV-2 spike protein with a truncation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-ELYS-emerin-EGFP-polyA

    Plasmid

    #59745

    Purpose

    Express ELYS-emerin-EGFP fusion protein in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    ELYS-emerin-EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5 FRT TO Myc ArC Long WT

    Plasmid

    #59809

    Purpose

    Allows the integration of Myc ArC Long in the genome and Tet-inducible expression.

    Depositor

    Stephen Taylor

    Article

    Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

    Insert

    Aurora C (AURKC Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    hybrid human cytomegalovirus (CMV)/TetO2 promoter

    Available since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(-) Full length Her2(ERBB2)-Fc

    Plasmid

    #194330

    Purpose

    Mammalian expression of full length Human epidermal growth factor (Her)-2-Fc fusion protein

    Depositor

    Alexander McLellan

    Insert

    Her2-Fc (ERBB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA 3.1 (+) FLAG-Ago2 S387E

    Plasmid

    #60653

    Purpose

    Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    CMV-FLAG-Ago2 (N-term tag)

    Expression

    Mammalian

    Mutation

    S387E mutant

    Promoter

    CMV

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. Nano-lantern(Ca2+)_300/pcDNA3

    Plasmid

    #51983

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=300nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-3GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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