We narrowed to 26,733 results for: STI

1. pnEA-vH_His-TEV-DSep1-msfGFP

   Plasmid

   #174494

   Purpose

   bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Insert

   Sep1 (Sep1 Fly)
   

   Tags

   His6-TEV and msfGFP

   Expression

   Bacterial

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SITS-Cavin4a-mCherry

   Plasmid

   #176022

   Purpose

   Leishmania cell free expression of zebrafish Cavin4a-mCherry. Parton lab clone GWU

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   Cavin4a (cavin4a Zebrafish)
   

   Use

   Leishmania cell free expression

   Tags

   mCherry

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. SITS-Cavin4a-EGFP

   Plasmid

   #176018

   Purpose

   Leishmania cell free expression of zebrafish Cavin4a-EGFP. Parton lab clone GRK

   Depositor

   Rob Parton

   Article

   Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

   Insert

   Cavin4a (cavin4a Zebrafish)
   

   Use

   Leishmania cell free expression

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2plus-TDP-43

   Plasmid

   #162608

   Purpose

   Allows for transcription of TDP-43 mRNA for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   TARDBP (TARDBP Human)
   

   Expression

   Mammalian

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR223-PARK7H126A

   Plasmid

   #115180

   Purpose

   Gateway cloning of PARK7H126A into any destination vector

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PARK7H126A (PARK7 Human)
   

   Use

   Gateway cloning into appropriate destination vect…

   Mutation

   p.H126A

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR223-PARK7E163K

   Plasmid

   #115181

   Purpose

   Gateway cloning of PARK7E163K into any destination vector

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PARK7E163K (PARK7 Human)
   

   Use

   Gateway cloning into appropriate destination vect…

   Mutation

   p.E163K

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR223-PARK7V51G

   Plasmid

   #115178

   Purpose

   Gateway cloning of PARK7V51G into any destination vector

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PARK7V51G (PARK7 Human)
   

   Use

   Gateway cloning into appropriate destination vect…

   Mutation

   p.V51G

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR223-PARK7C53A

   Plasmid

   #115179

   Purpose

   Gateway cloning of PARK7C53G into any destination vector

   Depositor

   Mohan Babu

   Article

   Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:

   Insert

   PARK7C53A (PARK7 Human)
   

   Use

   Gateway cloning into appropriate destination vect…

   Mutation

   p.C53A

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. ST-2xTstem(5A)-GFP

   Plasmid

   #162504

   Purpose

   Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   One Tac stem region is inserted within ST6Gal1 an…

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ST-2xTstem-GFP

    Plasmid

    #162503

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One Tac stem region is inserted within ST and the other Tac stem region is inserted between ST and GFP.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    One Tac stem region is inserted within ST6Gal1 an…

    Available Since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMY-CD90.2-P2A-BlastDYK

    Plasmid

    #163349

    Purpose

    Retroviral vector to express the surface marker CD90.2 and a DYK-tagged blasticidin resistance marker

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Insert

    CD90.2 (Thy1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. DOT1L-CC3

    Plasmid

    #111754

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Contains coiled coil #3 (residues 613-661)

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. DOT1L-CC1

    Plasmid

    #111756

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    contains coiled coil #1 (residues 506-555)

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. DOT1L-CC2

    Plasmid

    #111755

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Article

    Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

    Insert

    DOT1L (DOT1L Human)
    

    Tags

    8xHis-Thrombin-SUMO-TEV

    Expression

    Bacterial

    Mutation

    Contains coiled coil #2 (residues 560-608)

    Promoter

    T7

    Available Since

    June 14, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pcDNA3-CARMA2sh-delta458-500

    Plasmid

    #97074

    Purpose

    Expresses mutant CARD14/CARMA2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 458-500

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX313-TP53-WT

    Plasmid

    #118014

    Purpose

    Constitutive expression of wild-type human Tumor Protein p53 (TP53)

    Depositor

    David Root, William Hahn

    Article

    Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

    Insert

    TP53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. EF1a-KLF2-T2A-mCHERRY

    Plasmid

    #224295

    Purpose

    Expresses mouse KLF2 and mCherry under EF1a promoter

    Depositor

    Nikhil Joshi

    Article

    Fagerberg et al Science. 2025 Jan 2;387(6735):eadn2337.

    Insert

    KLF2 T2A mCherry (Klf2 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT3-EF1a-YapS127A

    Plasmid

    #46049

    Depositor

    Xin Chen

    Article

    Tschaharganeh et al Gastroenterology. 2013 Jun;144(7):1530-1

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    S127A

    Promoter

    EF1a

    Available Since

    July 2, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

    Plasmid

    #67974

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Douglas Kim, Loren Looger, GENIE Project

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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21. Tet-pLKO-puro-Scrambled

    Plasmid

    #47541

    Purpose

    Tet inducible scrambled shRNA.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    Scrambled shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. Tet-pLKO-puro-SOX2

    Plasmid

    #47540

    Purpose

    Tet inducible knockdown of SOX2.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    SOX2 shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKLV-EF1a-mCherryBCL11B-W

    Plasmid

    #208758

    Purpose

    Lentiviral vector expressing mCherry fused with BCL11B cDNA

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    BCL11B (BCL11B Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pT3-EF1A-CCNE1

    Plasmid

    #162553

    Purpose

    Overexpression of CCNE1

    Depositor

    Amaia Lujambio

    Article

    Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

    Insert

    Overexpression of CCNE1 (CCNE1 Human)
    

    Expression

    Mammalian

    Mutation

    None

    Promoter

    EF1A

    Available Since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pRetro-CAG-DsRedExpress-G-TVA

    Plasmid

    #235698

    Purpose

    Expresses G protein, TVA receptor, and DsRed for rabies virus trans-synaptic tracing

    Depositor

    Hongjun Song, Guo-li Ming

    Article

    Sun et al Nature. 2025 Jan 16. doi: 10.1038/s41586

    Insert

    DsRedExpress, TVA800, and Glyco
    

    Use

    Retroviral

    Promoter

    CAG

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLV-UbC-Tet1v4-dCas9

    Plasmid

    #232180

    Purpose

    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Insert

    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 Human, S. pyogenes)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    hUbC

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCAGGS puro HA-hUSP27X

    Plasmid

    #225718

    Purpose

    Bacterial expression of USP27X with HA tag

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X (USP27X Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid

    #67977

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLPC-dnMAML1-mVenus

    Plasmid

    #171023

    Purpose

    Retroviral vector for CMV promoter driven expression of dominant negative (DN) MAML1 (Notch pathway) fused to mVenus fluorescent protein (to be used in conjunction with Phoenix packaging cells).

    Depositor

    Masashi Narita

    Article

    Hoare et al Nat Cell Biol. 2016 Sep;18(9):979-92. do

    Insert

    MAML1 Dominant Negative (MAML1 Human)
    

    Use

    Retroviral

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    amino acids 12-74 only, hence conferring dominant…

    Promoter

    CMV

    Available Since

    April 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLVX-UbC-rtTA-Ngn2:2A:EGFP

    Plasmid

    #127288

    Purpose

    all-in-one inducible LV expression construct for Ngn2:2A:EGFP (aka. UNG; for direct neuronal conversion)

    Depositor

    Fred Gage

    Article

    Schafer et al Nat Neurosci. 2019 Feb;22(2):243-255. do

    Insert

    Ngn2:2A:Ascl1 (NEUROG2 Human)
    

    Use

    Lentiviral

    Tags

    Ngn2 and EGFP linked via 2A

    Expression

    Mammalian

    Available Since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Beta-2-adrenergic receptor-YFP

    Plasmid

    #67146

    Purpose

    Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    Beta-2-adrenergic receptor-YFP (ADRB2 Human, Aequorea victoria)
    

    Tags

    YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1(+)-C-6His-mEsrra

    Plasmid

    #173152

    Purpose

    mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells

    Depositor

    Brijesh Kumar Singh

    Article

    Tripathi et al bioRxiv 2021.11.16.468891

    Insert

    ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    Wild type

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTT3-ecdICAM1-ST3-His

    Plasmid

    #210666

    Purpose

    Expression of the extracellular domain of human ICAM1 fused to Spytag003 + Histag in HEK293 (or similar)

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Extracellular domain of human ICAM-1 fused to Spytag003 and Histag (ICAM1 Human, Synthetic)
    

    Tags

    Histag and Spytag003

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

    Plasmid

    #67979

    Purpose

    Cas9 activity reporter (control) with BFP and GFP.

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHR-EF1Alpha-puro-T2A-Tet-on 3G-TRE3G-Ascl1

    Plasmid

    #118593

    Purpose

    Expresses a puromycin resistance cassette with Tet-on 3G from the EF1Alpha promoter and Ascl1 from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    Puro-T2A-Tet-on 3G/Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1Alpha/TRE3G

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid

    #103866

    Purpose

    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Lenti-CMV-PEmax-IRES-ZeoR

    Plasmid

    #207353

    Purpose

    Lentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    Prime editor max (PEmax) enzyme
    

    Use

    Lentiviral

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

    Plasmid

    #67981

    Purpose

    Cas9 activity reporter (control) with mCherry and GFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

    Plasmid

    #67982

    Purpose

    Cas9 activity reporter with mCherry and GFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. AAV-CAG-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid

    #130909

    Purpose

    AAV-mediated expression of ChrimsonR-tdTomato under the CAG promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV8

    Insert

    ChrimsonR-tdTomato
    

    Use

    AAV

    Tags

    tdTomato (codon diversified version)

    Expression

    Mammalian

    Mutation

    Chrimson K176R mutant

    Promoter

    CAG

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge