We narrowed to 7,650 results for: RAP

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pSHUT4-eYFP

Plasmid

#133094

Purpose

Agrobacterium T-DNA overexpression cassette comprising constitutive TEF-1alpha fused to reporter eYFP. Integrates near beta-tubulin gene in F. solani genome. Replace eYFP w/ XhoI BamHI

Depositor

Jens Laurids Sørensen

Article

Nielsen et al Fungal Biol Biotechnol. 2019 Dec 11;6:25

Insert

enhanced yellow flourescent protein

Use

Fungal expression

Promoter

TEF-1α

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG IL13-CAR

Plasmid

#157742

Purpose

knock IL13-CAR into AAVS1 safe harbor locus for constitutive expression

Depositor

Xiaoping Bao

Article

Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

Insert

Chimeric antigen receptor targeting IL13

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pOGS539_GLuc-ns

Plasmid

#226719

Purpose

Plasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Gaussia Luciferase

Tags

HA tag

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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α-catenin ECFP-YPET FRET ΔF-actin binding site

Plasmid

#198245

Purpose

Alpha catenin FRET conformation sensor protein with C-terminal truncation removing ability to bind F-actin.

Depositor

Deborah Leckband

Article

Kim et al Curr Biol. 2015 Jan 19;25(2):218-24. doi

Insert

α-catenin ECFP-YPET FRET sensor ΔF-actin binding site

Tags

ECFP and YPET

Expression

Mammalian

Mutation

Deleted F-actin binding site on a-catenin (849-90…

Promoter

CMV

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ST-SBP-tagBFP

Plasmid

#65266

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pLC-RFP657-CASP8

Plasmid

#75164

Purpose

LentiCRISPR-RFP657 with sgRNA targeting human Caspase-8

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

CASP8 sgRNA (CASP8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-hRedOpsin-fCD200

Plasmid

#164425

Purpose

AAV plasmid expressing full-length CD200 in photoreceptors

Depositor

Connie Cepko

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

CD200 (Cd200 Mouse)

Use

AAV

Expression

Mammalian

Promoter

human red opsin

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-EMCV_3C

Plasmid

#201941

Purpose

Expresses EMCV 3C protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

EMCV 3C protease (EMCVgp1 )

Expression

Yeast

Promoter

GAL1

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLX304-MARK3_T211A

Plasmid

#107236

Purpose

Lentiviral gene expression vector for phospho-MARK3_T221A expression

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MARK3

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-HRV-B14_3C

Plasmid

#203466

Purpose

Expresses HRV-B14 3C protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HRV-B14 3C protease (HRVBgp1 )

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-MED27

Plasmid

#15424

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED27 (MED27 Human)

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
SBC009876

Plasmid

#226283

Purpose

Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

Depositor

Nigel Scrutton

Article

Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

Inserts

Coniferyl alcohol acyltransferase
Eugenol synthase 1

Expression

Bacterial

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SEMPER_ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry

Plasmid

#221081

Purpose

SEMPER plasmid for expression of msfGFP[r5M] and msfBFO[r5M] and emiRFP670 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-msfBFP[r5M]_ACC-msfGFP[r5M]_ACC-emiRFP670_IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCytERM-mGreenLantern

Plasmid

#164467

Purpose

Visualization of the ER in cells (compatible with OSER assay)

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Tags

Cytochrome p450 first 29 animo acids (Oryctolagus…

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBMN mCherry-p62(W340A/ΔUBD)

Plasmid

#119688

Purpose

For mammalian expression of LIR mutant p62 without its UBD

Depositor

Michael Lazarou

Article

Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

Insert

p62 (SQSTM1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

W340A & UBD deletion (1-385)

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLPC-FLAG.Mm.DMAP1

Plasmid

#184650

Purpose

Retroviral expression of mouse DMAP1

Depositor

Agnel Sfeir

Article

Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

Insert

Dmap1 (Dmap1 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pmKO-imp α

Plasmid

#200082

Purpose

Expresses mKO-tagged human importinα in mammalian cells

Depositor

Kenji Sugimoto

Article

Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

Insert

importin α (KPNA2 Human)

Tags

mKO

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe puro-HA-PIK3CA(H1047R;F486S;del)

Plasmid

#33255

Depositor

Thomas Roberts

Article

Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

changed histidine 1047 to arginine and phenylalan…

Promoter

SV40

Available Since

Jan. 4, 2012

Availability

Academic Institutions and Nonprofits only
mEGFP-N1-NF2

Plasmid

#166473

Purpose

Expresses fusion of mEGFP and NF2

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, NF2 (NF2 Human)

Expression

Mammalian

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-hMSN

Plasmid

#211823

Purpose

Express human MSN in mammalian cells

Depositor

Haian Fu

Article

Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

Insert

MSN (MSN Human)

Tags

GST tag

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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