-
Plasmid#52891PurposeAedes aegypti polyubiquitin promoter driving firefly luciferaseDepositorInsertpolyubiquitin promoter
UseLuciferaseTagsExpressionMutationPromoterAe aegypti polyubiquitinAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
His-Tev-WWP2
Plasmid#186807PurposeCodon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.DepositorInsertHis-Tev-WWP2 (WWP2 Human)
UseTagsHis6-TevExpressionBacterialMutationPromoterAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IE1
Plasmid#52894Purposebaculovirus IE-1 promoter driving firefly luciferaseDepositorInsertIE1 promoter
UseLuciferaseTagsExpressionMutationPromoterbaculovirus IE1Available sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-E6AP Hect
Plasmid#165102Purposebacterial expression of a GST fusion of Human E6AP Hect domainDepositorInsertE6AP Hect (UBE3A Human)
UseTagsGST from plasmid.ExpressionBacterialMutationE6AP Hect domain Amino acids 495-738 (5'QLNP…PromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateTagsExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-MCS-WPRE-UbC-mCherry
Plasmid#211799PurposeLentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing mCherry under the Ubiquitin C (UbC) promoterDepositorInsertsmultiple cloning site
mCherry
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-mCherry-WPRE-UbC-MCS
Plasmid#211801PurposeLentiviral vector plasmid expressing mCherry under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoterDepositorInsertsmCherry
multiple cloning site
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
V51 pIRESpuro-GLUE (pGLUE)
Plasmid#15100Purposetandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tagsDepositorTypeEmpty backboneUseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKH3-TRIM28
Plasmid#45569DepositorInsertTRIM28 (TRIM28 Human)
UseTags3xHAExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
FUG-P2A-tC3-W
Plasmid#129475Purpose3rd-generation lentiviral vector for hUbC-driven co-expression of EGFP and truncated C3DepositorInserttruncated (AA173-201) exoenzyme C3 from C. botulinum, P2A, EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterhuman ubiquitin CAvailable sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-MCS-WPRE-UbC-Emerald
Plasmid#211796PurposeLentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsmultiple cloning site
Emerald
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
T-TRIM33-4
Plasmid#166550PurposescFv of a human scaffold targeting E3 ubiquitin-protein ligase TRIM33. Antigen coverage aa 882-1127 of 1127DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-Emerald-WPRE-UbC-MCS
Plasmid#211800PurposeLentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and containing a multiple cloning site (MCS) under the Ubiquitin C (UbC) promoterDepositorInsertsEmerald
multiple cloning site
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-MCS-WPRE-UbC-MCS
Plasmid#211798PurposeLentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and another multiple cloning site (MCS) under the Ubiquitin C (UbC) promoterDepositorInsertsmultiple cloning site
multiple cloning site
UseLentiviralTagsExpressionMammalianMutationPromoterSFFV and UbCAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
RCAS IkB K->A
Plasmid#12408DepositorInsertIkB alpha K->A (Nfkbia Mouse)
UseRetroviralTagsExpressionMammalianMutationLysines 21, 22, and 67 are mutated to alanine. (T…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Tet-UPF1WT (pRKB264)
Plasmid#99146PurposeInducible lentiviral construct to express FLAG-tagged UPF1wtDepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationFirst 127 codons are optimized to reduce GC conte…PromoterpTightAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_HT_B_6xHis-TEV-GFP-NBR1
Plasmid#190928PurposePlasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914DepositorInsertNBR1 (NBR1 Human)
UseTagsStrep-TEV-GFPExpressionInsectMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEW3: pET-32a Dot1L(2-416)
Plasmid#124098PurposeDot1L(2-416) expression plasmidDepositorInsertDot1L(2-416) (DOT1L Human)
UseTagsTrx-His-TEVExpressionBacterialMutationPromoterT7Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pN_35S/mCitrine/P_UBQ10/Derlin1-mOrange2
Plasmid#118000PurposeCo-expression of mCitrine under the control of the CaMV 35S promoter and Derlin1 fused to mOrange2 (an ER-targeting marker protein) under the control of the A. thaliana ubiquitin-10 promoter.DepositorInsertDerlin1-mOrange2
UsePlant/bacteria binary vectorTagsExpressionPlantMutationPromoterUbiquitin-10Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEW4: pET-32a Dot1L(2-386)
Plasmid#124099PurposeDot1L(2-386) expression plasmidDepositorInsertDot1L(2-386) (DOT1L Human)
UseTagsTrx-His-TEVExpressionBacterialMutationPromoterT7Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
GST-3C-FIP200-GFP
Plasmid#187832PurposePlasmid for the expression and purification of GST-3C-FIP200-GFP. Internal reference: SMC1445DepositorInsertFIP200 (RB1CC1 Human)
UseTagsGST and mGFPExpressionBacterial and InsectMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST15-Nedd4.2F823A
Plasmid#165104Purposebacterial expression of a GST fusion of Human Nedd4.2d F823ADepositorInsertNedd4.2d F823A (NEDD4L Human)
UseTagsGST from plasmid.ExpressionBacterialMutationF823A; mutation for destabilization of trimer fo…PromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-optimizedNedd4.2F823D
Plasmid#165105Purposebacterial expression of a GST fusion of Human Nedd4.2d F823DDepositorInsertNedd4.2d F823D (NEDD4L Human)
UseTagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expression; F823D; …PromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-optimized E6AP F727D
Plasmid#165101Purposeecoli optimized expression of a GST fusion of Human E6AP F727DDepositorInsertE6AP F727D (UBE3A Human)
UseTagsGST from plasmid.ExpressionBacterialMutationcodon optimized for E. coli expressionPromoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
USP08 (3N3K)
Plasmid#28115PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertUSP08 (USP8 Human)
UseTagsHis tag with TEV siteExpressionBacterialMutationCatalytic domain only.PromoterAvailable sinceFeb. 28, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
UBC44
Plasmid#25468PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertubc44.001.167
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
Pv-UBC
Plasmid#25592PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPv-UBC
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-N-Myc_1-205 USP7
Plasmid#131245PurposeMammalian expression of the USP7 TRAF-like domain with an N-terminal Myc tag.DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationExpresses a mutant form of our pcDNA3.1-N-Myc_1-5…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_535-888 USP7
Plasmid#131248PurposeMammalian expression of the USP7 UBL1-3 domains with an N-terminal Myc tag.DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_535-775 USP7
Plasmid#131247PurposeMammalian expression of the USP7 UBL1-2 domains with an N-terminal Myc tag.DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-1102 USP7…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DW164AA 1-205 USP7
Plasmid#131254PurposeMammalian expression of a mutant USP7 TRAF-like domain (DW164AA), with an N-terminal Myc tagDepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
UseTagsMycExpressionMammalianMutationExpresses a mutant form of our pcDNA3.1-N-Myc_1-2…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV3FLAG_hGTPBP4
Plasmid#53974PurposeMammalian expression plasmid for human GTPBP4 with n-terminal 3xFLAG epitope fused.DepositorInsertGTPBP4 (GTPBP4 Human)
UseTags3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
sAB-MBP
Plasmid#204736Purposesynthetic antigen-binding fragment specifically recognizing bacteria maltose-binding proteinDepositorInsertsynthetic antigen-binding fragment recognizing bacteria maltose-binding protein
UseTagsExpressionMutationPromoterAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-p62-LIR-mutant (LIRAAAA)
Plasmid#187981PurposePlasmid for the expression of a p62 mutant (LIR mutant: p62 3xDW + silent mutation). Internal reference: SMC528DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationDDDW335-338AAAA (LIR mutant), silent mutation siR…PromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)
Plasmid#99144PurposeInducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139ADepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationFirst 127 codons are optimized to reduce GC conte…PromoterpTightAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
HIS-HA-CMYC P53
Plasmid#233738Purposeexpresses HIS-HA-CMYC triple tagged P53 for pulldown assayDepositorInsertHIS-HA-CMYC-p53 (TP53 Human)
UseTagsHIS-HA-CMYCExpressionBacterialMutationPromoterT7Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
p667-UBC-GFP-V5_IDG-K
Plasmid#135242PurposeGateway destination clone of GFP (as control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertGFP-V5
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianMutationPromoterUbiquitinAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - mcp - egfp - BirA*
Plasmid#131134PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) monomer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-MCP-EGFP-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
phage - ubc - nls - 2xmcp - mCherry - BirA*
Plasmid#131136PurposeBirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and mCherry; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.DepositorInsertNLS-HA-2xMCP-mCherry-BirA*
UseLentiviralTagsNLS, HAExpressionMammalianMutationBirA* = BIrA >R118GPromoterhuman ubiquitin C (UBC)Available sinceAug. 30, 2019AvailabilityAcademic Institutions and Nonprofits only