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Showing: 101 - 150 of 176 results

  6. pLC025 - pL0_StopCodon (CT)

    Plasmid
    #203905
    Purpose
    MoClo compatible L0 plasmid containing a stop codon. To allow for proper fusion with CDS1ns L0 plasmids, this plasmid adds an additional glycine and serine to the C-terminal end of a CDS and then adds a stop codon to terminate translation.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d
    Insert
    Stop codon
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    203905-plasmid-map-sequence-id-404856

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  12. RIP7-RLuc-YFP-pBS

    Plasmid
    #72482
    Purpose
    Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals
    Depositor
    Vincent Poitout
    Article
    Ghislain et al Endocrinology. 2015 Dec 15:en20151550.
    Inserts
    rat ins2 gene promoter (Ins2 Rat)
    luciferase
    eYFP
    Use
    Luciferase
    Tags
    eYFP and renilla luciferase
    Expression
    Mammalian
    Mutation
    eYFP varient
    Promoter
    Available since
    March 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    72482-plasmid-map-sequence-id-136970

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  17. pSB278 - pL0_pT16H2 (pro + 5U)

    Plasmid
    #203889
    Purpose
    T16H2 promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d
    Insert
    T16H2 promoter and 5'UTR
    Use
    Tags
    Expression
    Plant
    Mutation
    Mutation of BpiI or BsaI cut site for MoClo domes…
    Promoter
    Available since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    203889-plasmid-map-sequence-id-404811

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  21. pJET-col2a1a-KI-donor

    Plasmid
    #184876
    Purpose
    Donor template for knock-in of p2a-CreERT2 into the medaka col2a1a locus via CRISPR/Cas9-mediated homology directed repair
    Depositor
    Christoph Winkler
    Article
    Hui et al Development. 2022 May 20. pii: 275483. d
    Inserts
    Collagen2a1a 5' homology arm
    Collagen2a1a 3' homology arm
    p2a-CreERT2
    Myosin light chain 2 promoter
    EGFP
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    184876-plasmid-map-sequence-id-362292

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  25. pUN1213 - pL0_p16OMT (pro + 5U)

    Plasmid
    #203890
    Purpose
    16OMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.
    Depositor
    Carolyn Lee-Parsons
    Article
    Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d
    Insert
    16OMT promoter and 5'UTR
    Use
    Tags
    Expression
    Plant
    Mutation
    Mutation of BpiI and/or BsaI cut sites for MoClo …
    Promoter
    Available since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    203890-plasmid-map-sequence-id-404813

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  39. mEmerald-Centrin2-N-10

    Plasmid
    #54039
    Purpose
    Localization: Centrosomes, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Centrin2 (CETN2 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. mCherry-Centrin2-N-10

    Plasmid
    #55018
    Purpose
    Localization: Centrosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Centrin2 (CETN2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    55018-plasmid-map-sequence-id-371039

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  41. mApple-Centrin2-N-10

    Plasmid
    #54878
    Purpose
    Localization: Centrosomes, Excitation: 568, Emission: 592
    Depositor
    Michael Davidson
    Insert
    Centrin2 (CETN2 Human)
    Use
    Tags
    mApple
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. tdEos-Centrin2-N-10

    Plasmid
    #57599
    Purpose
    Localization: Centrosomes, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Centrin2 (CETN2 Human)
    Use
    Tags
    tdEos
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  43. pCB99

    Plasmid
    #69745
    Purpose
    Retroviral expression of Centrin 2
    Depositor
    Iain Cheeseman
    Insert
    Centrin 2 (CETN2 Human)
    Use
    Retroviral
    Tags
    eGFP-TEV-S
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  44. pCI-Puro_hCOL4A2-Myc-His-WPRE

    Plasmid
    #198080
    Purpose
    Express hCOL4A2 in mammalian cells
    Depositor
    Jeffrey Miner
    Insert
    COL4A2 (COL4A2 Human)
    Use
    Tags
    TEV-Myc-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, beta-globin/IgG chimeric intron
    Available since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    198080-plasmid-map-sequence-id-390107

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Showing: 101 - 150 of 176 results