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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 22,202 results for: ESC

Showing: 101 - 150 of 22202 results

  9. pBS-L1PA1CH_blast rescue

    Plasmid
    #69611
    Purpose
    Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts
    Depositor
    Astrid Roy-Engel
    Insert
    L1PA1
    Use
    Tags
    blast rescue cassette
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    69611-plasmid-map-sequence-id-123344

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  10. pBluescriptSKII+ U6-sgRNA(F+E) ACTB

    Plasmid
    #74705
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA
    Depositor
    Eugene Yeo
    Article
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    74705-plasmid-map-sequence-id-151422

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  11. Rescue Septin 11 mRNA

    Plasmid
    #122022
    Purpose
    Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.
    Depositor
    Angel de Blas
    Article
    Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6
    Insert
    SEPT11 (Sept11 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Silent mutations are in 5 consecutive codons (AUU…
    Promoter
    Available Since
    Feb. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122022-plasmid-map-sequence-id-235846

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  12. pESC-NAT-LACap

    Plasmid
    #80763
    Purpose
    Expresses truncated L-A capsid for curing satellite virus
    Depositor
    John McCusker
    Insert
    L-A cDNA (L-A Cap)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PGK1
    Available Since
    Aug. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    80763-plasmid-map-sequence-id-156222

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  15. peSCBE3-NG-HF1-Hypa

    Plasmid
    #218166
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Tags
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218166-plasmid-map-sequence-id-433270

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  16. pBluescriptSKII+ U6-sgRNA(F+E) TFRC

    Plasmid
    #74709
    Purpose
    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of TFRC mRNA
    Depositor
    Eugene Yeo
    Article
    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30
    Insert
    U6 promoter driving sgRNA targeting the 3'UTR of TFRC mRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available Since
    April 27, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. pBluescript mouse ptc

    Plasmid
    #58699
    Purpose
    full length mouse ptc cDNA
    Depositor
    Matthew Scott
    Insert
    ptc (Ptch1 Mouse)
    Use
    Cloning vector
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available Since
    Aug. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  29. pBluescript alpha-SSeCKS

    Plasmid
    #61295
    Purpose
    contains rat alpha SSeCKS cDNA
    Depositor
    Irwin Gelman
    Insert
    alpha SSeCKS (Akap12 Rat)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    cDNA with 1kb of 3' UTR and 150bp of 5'…
    Promoter
    T7
    Available Since
    Dec. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 101 - 150 of 22202 results