Addgene

We narrowed to 85 results for: CYN

Showing: 41 - 60 of 85 results

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pUNIV backbone

Plasmid

#24705

Depositor

Cynthia Czajkowski

Article

Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

Type

Empty backbone

Use

Xenopus oocyte expression

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Available since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pAC-EsaR

Plasmid

#47643

Purpose

wt esaR gene under control of Plac in pACYC184

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

Plac-esaR-terminator

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
H2BK120C

Plasmid

#75332

Purpose

Bacteria expression of H2BK120C

Depositor

Cynthia Wolberger

Article

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

H2BK120C (h2bc11.S Frog)

Use

Tags

none

Expression

Bacterial

Mutation

truncated; K120C

Promoter

T7

Available since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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UBC13-PGEX4T

Plasmid

#25464

Depositor

Cynthia Wolberger

Article

Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

Insert

Ubc13 (UBC13, MMS2 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pAC-EsaR-D91G

Plasmid

#47646

Purpose

pAC-EsaR with a a272g mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-D91G

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Changed Aspartic acid 91 to Glycine

Promoter

Plac

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
hOtub1

Plasmid

#26959

Depositor

Cynthia Wolberger

Article

Wang et al J Mol Biol. 2009 Mar 6. 386(4):1011-23.

Insert

Otub1 (OTUB1 Human)

Use

Tags

His6 (TEV cleavable)

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2011

Availability

Academic Institutions and Nonprofits only
pCS-PluxIlux

Plasmid

#47641

Purpose

wt luxI promoter in pCS26

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

PluxI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PluxI

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
pAC-EsaR-V220A

Plasmid

#47645

Purpose

pAC-EsaR with a c659t mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-V220A

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Changed Valine 220 to Alanine

Promoter

Plac

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pET-PesaR-Clux

Plasmid

#47658

Purpose

PesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)

Depositor

Cynthia Collins

Article

Shong et al ACS Synth Biol. 2013 Jul 23.

Insert

PesaR-C

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Additional esa box between -35 site and the origi…

Promoter

PesaR-C

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only
Ubp8/PET32a

Plasmid

#26965

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Ubp8 (UBP8 Budding Yeast)

Use

Tags

His6 and Trx

Expression

Bacterial

Mutation

TEV site is added t the 5' end and 4 alanine…

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pAC-EsaR-I70V

Plasmid

#47663

Purpose

pAC-EsaR with a208g mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-I70V

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Changed Isoleucine 70 to Valine

Promoter

Plac

Available since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
UbG75C

Plasmid

#37194

Depositor

Cynthia Wolberger

Article

Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

Insert

Ubiquitin G75C mutant (RBX1 Human)

Use

Tags

No tag

Expression

Bacterial

Mutation

Glycine 75 changed to Cystine

Promoter

T7

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
Sgf73(1-106)/PET32a

Plasmid

#26962

Depositor

Cynthia Wolberger

Article

Samara et al Science. 2010 May 21. 328(5981):1025-9.

Insert

Sgf73(1-106) (SGF73 Budding Yeast)

Use

Tags

His6 and Trx

Expression

Bacterial

Mutation

with TEV site to cleave the N-terminal his+Trx tag

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pA9HD

Plasmid

#8588

Depositor

Cynthia Wolberger

Article

LaRonde-LeBlanc et al Genes Dev 2003 Aug 15;17(16):2060-72.

Insert

HoxA9 190-271with hexapeptide (HOXA9 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pRS423-GPD-wrmScarlet1-10-CYC1 terminator

Plasmid

#158584

Purpose

Expresses wrmScarlet1-10 in S. cerevisiae

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet1-10

Use

Tags

Expression

Yeast

Mutation

Promoter

GPD

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJG100

Plasmid

#138966

Purpose

Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet1-10

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 WT-pcDNA3.1

Plasmid

#118209

Purpose

Expresses FLAG-OTUB1 WT in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

none

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pP3-GFP

Plasmid

#53436

Purpose

Controls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genome

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

P3 promoter

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

none

Promoter

P3

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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HA-UBE2E1 C131A in pcDNA3.1

Plasmid

#118214

Purpose

Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

changed cysteine 131 to alanine

Promoter

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCS-PesaRlux

Plasmid

#47640

Purpose

wt esaR promoter in pCS26

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

PesaR

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PesaR

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge