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Showing: 1161 - 1200 of 1734 results

  2. FHL-cpmTq2-Calcium-lifetime-sensor

    Plasmid
    #129628
    Purpose
    Genetically encoded calcium sensor (GECI) that reports with lifetime and intensity changes
    Depositor
    Dorus Gadella
    Article
    van der Linden et al Nat Commun. 2021 Dec 9;12(1):7159. doi:
    Insert
    FHL-cpmTq2-Calcium-lifetime-sensor
    Use
    Tags
    6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV,&Rhamnose
    Available since
    June 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    129628-plasmid-map-sequence-id-260204

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  4. pDisplay-EGFP-CAAX

    Plasmid
    #208683
    Purpose
    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in mammalian cells
    Depositor
    Yulong Li
    Article
    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.
    Insert
    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    208683-plasmid-map-sequence-id-412176

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  5. pGLO-GFP-3UAG

    Plasmid
    #82501
    Purpose
    Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153
    Depositor
    Chang Liu
    Article
    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542
    Insert
    Green Fluorescence Protein with 3 UAG codons
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Added an UAG codon at the amino acid position 3, …
    Promoter
    AraBAD
    Available since
    Sept. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    82501-plasmid-map-sequence-id-156568

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  7. EphA2-MT

    Plasmid
    #108851
    Purpose
    Encodes for human EphA2 fluorescently labeled with mTurquoise on the C-Terminus via a 15 amino acid (GGS)5 flexible linker
    Depositor
    Kalina Hristova
    Article
    Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.
    Insert
    EPHA2 (EPHA2 Human)
    Use
    Tags
    Labeled with mTurquoise on the C-Terminus via a 1…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    108851-plasmid-map-sequence-id-208238

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  8. aP2-GFPtpz

    Plasmid
    #114211
    Purpose
    aP2/Fabp4 promoter driving GFPtopaz for labeling differentiated adipocytes and macrophage
    Depositor
    David Rowe
    Article
    Case et al Bone. 2013 Jan;52(1):454-64. doi: 10.101
    Insert
    aP2 promoter + pOB4 + eGFPtopaz (Fabp4 Aequorea victoria, Mouse, Synthetic)
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    Promoter
    aP2/Fabp4
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    114211-plasmid-map-sequence-id-220086

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  10. Cer(1-158)-RGS7

    Plasmid
    #55760
    Purpose
    An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    mCer(1-158)-RGS7 (RGS7 Aequorea victoria, Human)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    RGS7 was amplified via PCR, which added an N-term…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55760-plasmid-map-sequence-id-115314

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  11. pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA

    Plasmid
    #154775
    Purpose
    plasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    150TAA in GFP reporter, UUA anticodon and G10A, …
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154775-plasmid-map-sequence-id-310415

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  12. RIP7-RLuc-YFP-pBS

    Plasmid
    #72482
    Purpose
    Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals
    Depositor
    Vincent Poitout
    Article
    Ghislain et al Endocrinology. 2015 Dec 15:en20151550.
    Inserts
    rat ins2 gene promoter (Ins2 Rat)
    luciferase
    eYFP
    Use
    Luciferase
    Tags
    eYFP and renilla luciferase
    Expression
    Mammalian
    Mutation
    eYFP varient
    Promoter
    Available since
    March 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    72482-plasmid-map-sequence-id-136970

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  14. pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA

    Plasmid
    #154776
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    102TAG 150TAA in GFP reporter, hybrid PylT with A…
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154776-plasmid-map-sequence-id-310409

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  15. pAS_4xhyb PylT A41AA C55A sfGFP 150TAG

    Plasmid
    #154772
    Purpose
    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    150TAG in GFP reporter, hybrid PylT with A41AA an…
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154772-plasmid-map-sequence-id-310518

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  16. pCSGFPnERT(#205)

    Plasmid
    #184065
    Purpose
    cyclofen-inducible GFP nuclear relocation via mammalian cell transfection or mRNA synthesis
    Depositor
    Sophie Vriz
    Article
    Sinha et al Chembiochem. 2010 Mar 22;11(5):653-63. d
    Insert
    eGFP-nls-ERT2
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    sCMV+SP6
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184065-plasmid-map-sequence-id-365701

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  17. pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAG

    Plasmid
    #154777
    Purpose
    plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration
    Depositor
    Simon Elsaesser
    Article
    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    102TAA 150TAG in GFP reporter, hybrid PylT with …
    Promoter
    EF1
    Available since
    Oct. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    154777-plasmid-map-sequence-id-310408

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  19. pDONR P4-P1R-EGFP

    Plasmid
    #48348
    Purpose
    Contributes the coding sequence for EGFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Tomas Santalucia, Anna Planas
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    EGFP
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Contains a Kozak sequence. Does not contain a sto…
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48348-plasmid-map-sequence-id-70559

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  21. pCS2+ cMyc-oDam_f_GFPoNLS

    Plasmid
    #85818
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    cMyc oDam_f_GFPoNLS
    Use
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Promoter
    Available since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85818-plasmid-map-sequence-id-169572

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  22. pRD439

    Plasmid
    #168465
    Purpose
    For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
    Depositor
    Remus Dame
    Article
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS-mTurquoise2-H-NSdbd-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168465-plasmid-map-sequence-id-331915

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  23. pcDNA4/TO-bio-myc-NES-EGFP

    Plasmid
    #112712
    Purpose
    For tetracycline-inducible expression of bio-myc-NES-EGFP in mammalian cells
    Depositor
    Daniel Schoenberg
    Article
    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1
    Insert
    EGFP
    Use
    Tags
    HIV Rev nuclear localization signal (NES), biotin…
    Expression
    Mammalian
    Mutation
    Nucleotide sequence optimized for synthetic gene …
    Promoter
    CMV with Tet repressor binding sites
    Available since
    May 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    112712-plasmid-map-sequence-id-217058

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  31. pCMV-lyso-pHoenix

    Plasmid
    #70112
    Purpose
    Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes
    Depositor
    Christian Rosenmund
    Article
    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n
    Insert
    lyso-pHoenix (Cd63 Mouse, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
    Use
    Tags
    Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    70112-plasmid-map-sequence-id-418643

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  35. pAAV2-SynTetOff-FLEX-[FGL-2A-palmRFP1]

    Plasmid
    #190236
    Purpose
    AAV vector, high-level expression of somatodendritic-targeted EGFP (FGL) and membrane-targted mRFP1 (palmRFP1) in neuronal cells in the presence of Cre recombinase
    Depositor
    Hiroyuki Hioki
    Article
    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi
    Insert
    EGFP
    Use
    AAV
    Tags
    F2A, LDLR C-terminal sequence, mRFP1, myristoylat…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    190236-plasmid-map-sequence-id-381571

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  38. pAAV.hSyn.FLEX.SYFP2-POST-T2A-dTomato.FLEX.WPRE.SV40

    Plasmid
    #105983
    Purpose
    This AAV plasmid in the presence of Cre recombinase expresses an extracellular SYFP2 fused to the neuroligin-1 cytoplasmic domain for targeting to the synapse with a cell fill dTomato.
    Depositor
    Marcel Bruchez
    Article
    Kuljis et al eNeuro. 2019 Sep 23. pii: ENEURO.0193-19
    Insert
    FLEX-SYFP2-POST-T2A-dTomato.FLEX
    Use
    AAV and Cre/Lox
    Tags
    SYFP2-POST, T2A-dTomato, and myc
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin 1
    Available since
    Oct. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    105983-plasmid-map-sequence-id-235283

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Showing: 1161 - 1200 of 1734 results