We narrowed to 4,631 results for: lum

1. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

   Plasmid

   #115698

   Purpose

   ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae

   Depositor

   Eckhard Boles

   Article

   Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10

   Insert

   SSKar2-sfpHluorin-HDEL
   

   Use

   Tags

   ER retention signal containing HDEL and ER signal…

   Expression

   Yeast

   Mutation

   for introduced mutations see publication

   Promoter

   MET25

   Available since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSB700 lenti gRNA EYFP

   Plasmid

   #167906

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMuLE_ENTR_CBF-tdTomato_L3-L2

   Plasmid

   #113708

   Purpose

   Single pathway reporter; notch promoter-driven expression of tdTomato; MultiSite Gateway entry vector.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Insert

   CBF (notch dependent promoter)
   

   Use

   Mule gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Prab-3::NLS::GCaMP6s

   Plasmid

   #68119

   Purpose

   Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

   Depositor

   Andrew Leifer

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

   Insert

   rab-3::NLS::GCaMP6s
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rab-3

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pluc2-iRFP720

   Plasmid

   #98887

   Purpose

   Mammalian expression

   Depositor

   Laura Mezzanotte

   Article

   Mezzanotte et al Cell Transplant. 2017 Apr 26. doi: 10.37

   Insert

   Firefly Luciferase
   

   Use

   Tags

   iRFP720

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV_HaloK73T_cyto

   Plasmid

   #183687

   Purpose

   Mammalian expression of cytosolic metastable Halotag/folding sensor (Cyto HaloK73T)

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Insert

   Cyto Halo(K73T)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K73T in Halotag

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSB700 lenti gRNA Blasto_zhang2.0

   Plasmid

   #167912

   Purpose

   Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWXLd-RIEP-CeNLuc

   Plasmid

   #135934

   Purpose

   Expresses the CeNLuc LumiFluor in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   CeNLuc LumiFluor
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

   Plasmid

   #185678

   Purpose

   Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-HaloTag-KKMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLG1-puro Non-targeting sgRNA 3

    Plasmid

    #109003

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. mEmerald-MAP4-C-10

    Plasmid

    #54152

    Purpose

    Localization: Microtubules, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Article

    Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

    Insert

    MAP4
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    aa 640-1045 of MAP4

    Promoter

    CMV

    Available since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pMC-6-Cre-GR-9

    Plasmid

    #197723

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    Cre recombinase with St-LS1 intron fused to glucocorticoid receptor
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pL2-1 ConjEpi TpEF2nat TpEF2NoSQEVenusThrFLAG

    Plasmid

    #139961

    Purpose

    Over-expression of heterologus squalene epoxidase from Nannochloropsis oceanica

    Depositor

    Raffaela Abbriano

    Article

    Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

    Insert

    ConjEpi TpEF2_nat_tTpEF2 TpEF2_NoSQEVenusThrFLAG_tTpEF2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCS2-H2B-Dendra

    Plasmid

    #140559

    Purpose

    Red to green photoconvertible chromosome marker

    Depositor

    Heidi Hehnly

    Article

    Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

    Insert

    H2B
    

    Use

    Tags

    Dendra

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. NAX_CAGGS_CAS9_Puro

    Plasmid

    #167863

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSB700 lenti gRNA Puro_zhang2.0

    Plasmid

    #167919

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mNeonGreen-IETD-NanoLuc

    Plasmid

    #98288

    Purpose

    caspase-8 BRET Sensor

    Depositor

    Maarten Merkx

    Article

    den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

    Insert

    mNeonGreen-LEHD-NanoLuc
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-subtilase A

    Plasmid

    #35948

    Depositor

    Zhaoyang Feng

    Article

    Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

    Insert

    Subtilase A (subA E. Coli)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pLenti_CMV:Flag-GeNL_SV40:PuroR

    Plasmid

    #183040

    Purpose

    Expresses the FLAG-tagged GeNL (Green Nanolantern) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    GeNL Nanolantern
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWXLd-RIEP-GpNLuc

    Plasmid

    #135935

    Purpose

    Expresses the GpNLuc LumiFluor in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. XMRV vp62

    Plasmid

    #26189

    Depositor

    Joseph DeRisi

    Article

    Urisman et al PLoS Pathog. 2006 Mar . 2(3):e25.

    Insert

    XMRV VP62 (gag Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

22. pMOD_A-CmYLCV-dCas9-24XGP41

    Plasmid

    #202009

    Purpose

    Moclo level 1 - Module A, Promoter: CmYLCV, Gene: dCas9-24XGP41, Terminator: HSP

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Research, Volume 51, Issue

    Insert

    dCas9-24XGP41
    

    Use

    Synthetic Biology; Moclo level 1 vector

    Tags

    GS linkers

    Expression

    Mutation

    Promoter

    CmYLCV

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. BRAC/pcDNA3

    Plasmid

    #51967

    Purpose

    mammalian expression of BRAC, a BRET-based autofluorescent Ca2+ indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al PLoS One. 2010 Apr 1;5(4):e9935. doi: 10

    Insert

    BRAC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2014

    Availability

    Academic Institutions and Nonprofits only

24. SAC1deltaTMD-FKBP-mCherry

    Plasmid

    #108123

    Purpose

    Recruitable cytosolic SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    SACM1L(1-520):FKBP1A(3-108):mCherry (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(1-520)

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMC-6-WDV-Rep-RepA-9

    Plasmid

    #197716

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    WDV Rep/RepA proteins: native ORFs
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pMC-1-WDV-LS-L-11

    Plasmid

    #197719

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    WDV LSL in c-sense with GG expansion to v-sense ORF position
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. UAS-Cre-T2A-miRFP670

    Plasmid

    #193581

    Purpose

    Fluorescent reporter for Gal4-induced Cre recombination

    Depositor

    Satoru Takahashi

    Article

    Yoshihara et al Exp Anim. 2022 Aug 5;71(3):385-390. doi:

    Insert

    miRFP670
    

    Use

    Cre/Lox

    Tags

    T2A

    Expression

    Mutation

    Promoter

    5X UAS

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET28a-mCherry-2xEcMTS

    Plasmid

    #133624

    Purpose

    Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

    Insert

    mCherry-2xEcMTS
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCherry-SAC1-FKBP

    Plasmid

    #108129

    Purpose

    Recruitable SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:SACM1L:FKBP1A(3-108) (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NAX_CAGGS_CAS9_m4_Blasto

    Plasmid

    #167864

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMC-6-RedFLuc-9

    Plasmid

    #197738

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    RedF Luciferase (tomato optimized)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. LAMP1-ABKAR

    Plasmid

    #65068

    Purpose

    Expression of ABKAR biosensor targeted to the lysozome

    Depositor

    Jin Zhang, Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    ABKAR biosensor
    

    Use

    Tags

    Lamp1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

33. Nuc-ABKAR

    Plasmid

    #61511

    Purpose

    Expression of nuclear AMPK biosensor

    Depositor

    Jin Zhang, Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Use

    Tags

    NLS (see comments)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

34. pSB700 lenti gRNA Hygro

    Plasmid

    #167907

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSB700 lenti gRNA iRFP713

    Plasmid

    #167908

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Golgi-ABKAR

    Plasmid

    #61507

    Purpose

    Expression of AMPK biosensor targeted to the Golgi

    Depositor

    Jin Zhang, Takanari Inoue

    Article

    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

    Insert

    AMPK Biosensor
    

    Use

    Tags

    Golgi-targeting signal (see comments)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pRK5-Ufm1 WT

    Plasmid

    #133865

    Purpose

    Expression of human wild type UFM1

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    Ubiquitin-fold modifier 1 (UFM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDisplay-FRISZ

    Plasmid

    #176887

    Purpose

    Far-red fluorescent zinc indicator FRISZ in pDisplay

    Depositor

    Huiwang Ai

    Article

    Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

    Insert

    FRISZ
    

    Use

    Tags

    pDisplay backbone elements for export and membran…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGL3-p27Luc

    Plasmid

    #23047

    Depositor

    William Kaelin

    Article

    Zhang et al Nat Med. 2004 Jun . 10(6):643-8.

    Insert

    p27 (CDKN1B Human)
    

    Use

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Jan. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. phage UbiC SNAP-KDEL

    Plasmid

    #104982

    Purpose

    Lentiviral vector expressing SNAP-KDEL for ER labeling

    Depositor

    Jeffrey Chao

    Article

    Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

    Insert

    SNAP-KDEL
    

    Use

    Lentiviral

    Tags

    Secretion signal

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. hPDI1-18-508_C400S_C56S-pFLAG-CMV1

    Plasmid

    #31383

    Depositor

    David Ron

    Article

    Zito et al Mol Cell. 2010 Dec 10. 40(5):787-97.

    Insert

    PDI1A (P4HB Human)
    

    Use

    Tags

    FLAG M2

    Expression

    Mammalian

    Mutation

    C-terminal cysteines of both active sites mutated…

    Promoter

    Available since

    May 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. mCherry-FKBP-SAC1C389S

    Plasmid

    #108128

    Purpose

    Recruitable inactive SAC1

    Depositor

    Gerry Hammond

    Article

    Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

    Insert

    mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1LC389S (SACM1L Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SACM1L(C389S)

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pSB700 lenti gRNA iRFP713_zhang2.0

    Plasmid

    #167916

    Purpose

    Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMC-1-EXOB-11

    Plasmid

    #197688

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    EXOB enhancer-blocking insulator
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pminiTol2-4kb Wnt3 promoter-EGFP

    Plasmid

    #203792

    Purpose

    Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of EGFP for zebrafish transgenesis

    Depositor

    Thorsten Wohland

    Article

    Teh et al Development. 2015 Nov 1;142(21):3721-33.

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    4kb Wnt3 promoter

    Available since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pICH47732:PtFCP:ShBle

    Plasmid

    #104893

    Purpose

    Expresses ShBle with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation.

    Depositor

    Thomas Mock

    Article

    Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

    Insert

    ShBle
    

    Use

    Phaeodactylum; golden gate assembly

    Tags

    PtFCP promoter and terminator

    Expression

    Mutation

    Promoter

    Available since

    Dec. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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47. pLG1-puro Non-targeting sgRNA 1

    Plasmid

    #109002

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    Non-targeting sgRNA #1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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48. NAX_CAGGS_CAS9_mcherry

    Plasmid

    #167862

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pSB700 lenti gRNA EBFP2

    Plasmid

    #167905

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. NAX_CAGGS_CAS9_m4_Puro

    Plasmid

    #167871

    Purpose

    PiggyBac compatible plasmid expressing dCas9

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D10A, D839A, H840A, N863A

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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