We narrowed to 3,201 results for: Streptococcus

Showing: 581 - 600 of 3201 results

Results per page:

Sort results by:

pCAG-CFP-SaCas9-HF (without sgRNA)

Plasmid

#134470

Purpose

Human expression vector for SaCas9-HF variant.

Depositor

Zongli Zheng

Article

Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

Insert

Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity

Use

CRISPR

Tags

SV40NLS-3xFLAG

Expression

Mammalian

Mutation

R245A, N413A, N419A, R654A

Promoter

CAG

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBScomp Low

Plasmid

#223203

Purpose

Group B Streptococcus Low Expression Modular Complementation Vector

Depositor

Thomas Hooven

Article

Hillebrand et al Mol Microbiol. 2025 Nov;124(5):449-461.

Insert

N/A

Use

Complementation vector

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
LentiCRISPRv2-sgCTRL

Plasmid

#209750

Purpose

Lentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

Cas9

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-Dual-iCas9-EFS

Plasmid

#167501

Purpose

Expression of dual inducible Cas9 with EFS promoter

Depositor

Jake Liang

Article

Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

Insert

Cas9

Use

CRISPR, Lentiviral, and Luciferase

Tags

FLAG

Expression

Mammalian

Promoter

EFS

Available Since

Oct. 6, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
rGluA1 (px330:2-guideCas9)

Plasmid

#169437

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SiC-V1

Plasmid

#133041

Purpose

SiC-V1 vector with SpCas9 gene and dTomato reporter. sgRNA targeting a gene of interest can be cloned downstream of U6 promoter.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

SpCas9

Use

CRISPR and Lentiviral

Tags

dTomato

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TC9pAB

Plasmid

#71310

Purpose

used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

Depositor

Karl Degenhardt

Insert

Cas9

Use

In vitro transcription

Tags

NLS

Promoter

T7

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

Plasmid

#79619

Purpose

Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Tags

3xFlag-PmCDA1

Expression

Mammalian

Mutation

D10A for nickase Cas9

Promoter

pCMV

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Modular BE4max for GG insertion

Plasmid

#198891

Purpose

Easily insert deaminase of choice into BE4max using Golden Gate cloning

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

CRISPR associated protein 9 (cas9 Streptococcus pyogenes)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

Plasmid

#218508

Purpose

integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

mNeongreen

Tags

modified ssrA degradation tag

Expression

Bacterial

Mutation

deletion of MCS containing Plac_lacZa, introducti…

Promoter

P23

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
MSCV-Cas9-2A-BFP

Plasmid

#164662

Purpose

Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

Cas9
BFP

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

MSSV_LTR

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVNP2.0-SpCas9

Plasmid

#206880

Purpose

Expresses FLAG-tagged SpCas9 fused to the N-terminus of Gag/GagPol harbouring an intervening phospholipase C-δ1 pleckstrin homology (PH) domain

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

SpCas9 (cas9 )

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-1BPNLS-Cas9-1BPNLS

Plasmid

#87108

Purpose

BPNLS-Cas9-BPNLS expression plasmid

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

1BPNLS-Cas9-1BPNLS

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-iCas9

Plasmid

#160048

Purpose

inducible expression of Cas9

Depositor

Xiaojun Lian

Insert

Cas9

Expression

Mammalian

Promoter

TRE

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRRL-gRNA(anti-cGAS)-Cas9-Puro

Plasmid

#130908

Purpose

gRNA targeting human cGAS; Cas9 insert; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

Cas9

Expression

Mammalian

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28b-NmCas9-Flag

Plasmid

#109030

Purpose

Wild type Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant

Plasmid

#101213

Purpose

Bacterial expression plasmid for SpCas9 + K855A variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant K855A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

K855A

Promoter

T7

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pRel-Hyg

Plasmid

#227582

Purpose

Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Insert

Cas9

Use

CRISPR

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-MP-84-spCas9-U6-gRNA

Plasmid

#206935

Purpose

Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

Depositor

Markus Grompe

Insert

spCas9

Use

AAV

Tags

SV40 NLS

Expression

Mammalian

Promoter

MP-84

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
GluN2A (px330:2-guideCas9)

Plasmid

#169428

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge