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We narrowed to 6,501 results for: nls

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Showing: 581 - 600 of 6501 results

  1. pmAmetrine-DEVD-tdTomato

    Plasmid
    #18879
    Purpose
    Caspase-3 FRET biosensor, mammalian expression of mAmetrine-DEVD-tdTomato
    Depositor
    Robert Campbell
    Publication
    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mAmetrine-DEVD-tdTomato (CASP3 Human, lab constructed)
    Expression
    Mammalian
    Mutation
    mAmetrine-tdTomato linker sequence: ...ITLGGTGSGS…
    Available since
    Aug. 13, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    18879-plasmid-map-sequence-id-148222

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  3. pRD437

    Plasmid
    #168463
    Purpose
    For the insertion pf NLS-mEos3.2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS-mEos3.2-H-NSdbd-NLS
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168463-plasmid-map-sequence-id-331909

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  4. pRD443

    Plasmid
    #168469
    Purpose
    For the insertion pf NLS-mScarlet-I-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system
    Depositor
    Remus Dame
    Publication
    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414
    Insert
    NLS-mScarlet-I-H-NSdbd-NLS
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168469-plasmid-map-sequence-id-331930

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  5. pET28a/Cas9-Cys

    Plasmid
    #53261
    Purpose
    Expresses N-terminal His tag fused human codon-optimized Cas9 nuclease having C-terminal Cysteine
    Depositor
    Hyongbum Kim
    Publication
    Ramakrishna et al Genome Res. 2014 Apr 2.
    Insert
    Cas9-Cys
    Tags
    6X His tag, HA epitope, and NLS
    Expression
    Bacterial
    Mutation
    Additional Cysteine is added to the C-terminal re…
    Promoter
    T7 Promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    23 Articles
    53261-plasmid-map-sequence-id-261354

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  6. pC035 - dLwCas13a-msfGFP

    Plasmid
    #91925
    Purpose
    Expresses catalytically inactive LwCas13a for mammalian RNA targeting
    Depositor
    Feng Zhang
    Publication
    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLwCas13a
    Use
    CRISPR
    Tags
    NLS-3xHA and NLS-msfGFP
    Expression
    Mammalian
    Mutation
    R488A and R1060A
    Promoter
    EF-1a
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    91925-plasmid-map-sequence-id-228361

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  7. pCMV-PE7-P2A-GFP

    Plasmid
    #222996
    Purpose
    Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker
    Depositor
    Brittany Adamson
    Publication
    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE7-P2A-EGFP
    Tags
    P2A-EGFP, SV40 bpNLS, and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222996-plasmid-map-sequence-id-442553

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  8. Sa-dCas9-NLS-3xFLAG/pcDNA3.1

    Plasmid
    #98041
    Purpose
    Expresses Sa-dCas9-NLS-3xFLAG in mammalian cells for enChIP analysis to purify specific genomic regions of interest
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sa-dCas9-NLS-3xFLAG
    Use
    CRISPR
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    D10A + H557A
    Promoter
    CMV
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    98041-plasmid-map-sequence-id-398173

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  11. pLSC-5

    Plasmid
    #62889
    Purpose
    Lenti vector for expression of inducible split Cas9
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Inserts
    SpCas9(574-1368)
    SpCas9(2-573)
    Use
    CRISPR and Lentiviral
    Tags
    FFBP12, FRB, NES PTK2, NLS SV40, and P2A
    Expression
    Mammalian
    Mutation
    *see comment below
    Promoter
    EFS
    Available since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    62889-plasmid-map-sequence-id-105912

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  13. pcDNA3.1-hLbCpf1(TYCV) (pY230)

    Plasmid
    #89355
    Purpose
    Expresses humanized LbCpf1 variant that recognizes TYCV PAMs
    Depositor
    Feng Zhang
    Publication
    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cpf1, RR variant
    Use
    CRISPR
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Mutation
    G532R/K595R
    Promoter
    CMV
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    89355-plasmid-map-sequence-id-178226

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  14. pCRCT

    Plasmid
    #60621
    Purpose
    Plasmid encoding iCas9, tracrRNA and crRNAs
    Depositor
    Huimin Zhao
    Publication
    Bao et al ACS Synth Biol. 2014 Sep 19.
    Inserts
    iCas9
    tracrRNA
    Use
    CRISPR
    Tags
    FLAG and SV40 NLS
    Expression
    Yeast
    Mutation
    changed Aspartate 147 to Tyrosine, Proline 411 to…
    Promoter
    RPR1p and TEF1p
    Available since
    Oct. 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    18 Articles
    60621-plasmid-map-sequence-id-208224

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  15. PX404 Campylobacter jejuni Cas9

    Plasmid
    #68338
    Purpose
    Campylobacter jejuni Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    HA-NLS and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    7 Articles
    68338-plasmid-map-sequence-id-119561

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  16. pMJ920

    Plasmid
    #42234
    Depositor
    Jennifer Doudna
    Publication
    Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9
    Use
    CRISPR
    Tags
    GFP, HA epitope, and NLS (nuclear localization si…
    Expression
    Mammalian
    Mutation
    codon-optimized synthetic DNA sequence
    Promoter
    CMV
    Available since
    March 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    42 Articles
    42234-plasmid-map-sequence-id-57040

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  20. XW52

    Plasmid
    #65838
    Purpose
    XW52 Pmig-13-Czipper-QF-AD-NLS-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method
    Depositor
    Kang Shen
    Publication
    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi
    Insert
    Czipper-QF-AD-NLS
    Tags
    mCherry
    Expression
    Worm
    Promoter
    Pmig-13
    Available since
    Oct. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    65838-plasmid-map-sequence-id-120568

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Showing: 581 - 600 of 6501 results