We narrowed to 6,177 results for: nls

1. pEF-APEX2-NLS

   Plasmid

   #140281

   Purpose

   Expression of APEX2-NLS

   Depositor

   Yusuke Miyanari

   Article

   Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

   Insert

   APEX2-NLS
   

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF1a-hMLH1NTD-NLS

   Plasmid

   #174826

   Purpose

   Mammalian expression of human MLH1NTD-NLS (codon optimized)

   Depositor

   David Liu

   Article

   Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

   Insert

   human MLH1_NTD-NLS (codon optimized)
   

   Tags

   SV40 bpNLS

   Expression

   Mammalian

   Mutation

   truncation of residues 1-335

   Promoter

   EF1a

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA_Lifeact-GFP_NLS-mCherry

   Plasmid

   #69058

   Purpose

   Dual Fluorescent Reporter for F-actin and Nuclei

   Depositor

   Olivier Pertz

   Insert

   LifeAct-GFP_IRES_NLS-mCherry
   

   Tags

   Lifeact-GFP, NLS-mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFUGW NLS-FlpO

   Plasmid

   #183448

   Purpose

   Lentiviral FlpO vector

   Depositor

   Harold MacGillavry

   Article

   Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

   Insert

   NLS-FlpO
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGWT35S-Citrine-NLS

   Plasmid

   #182583

   Purpose

   Transient expression of Citrine-NLS in plant cell (Nucleus)

   Depositor

   Yutaka Kodama

   Article

   Osaki et al PeerJ. 2017 Sep 7;5:e3779. doi: 10.7717/

   Insert

   NLS
   

   Tags

   Citrine

   Expression

   Plant

   Promoter

   CaMV 35S promoter

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUAS-mCherry-NLS

   Plasmid

   #87695

   Purpose

   Gal4VP16 driven expression of mCherry-NLS in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   mCherry-NLS
   

   Tags

   nuclear localization signal

   Expression

   Mammalian

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. dCas9-AsLOV2x3-NLSx3

   Plasmid

   #231566

   Purpose

   Expresses components of the LOOMINA optogenetic transcriptional control system.

   Depositor

   Dominik Niopek

   Article

   Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

   Insert

   dCas9-AsLOV2x3
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMHE-NLS-mEGFP

   Plasmid

   #105527

   Purpose

   T7 promotor drives in vitro mRNA transcription of a mEGFP with a nuclear localisation signal

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   Nucleoplasmin
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Mutation

   NLS of nucleoplasmin (encoding amino acids KRPAAT…

   Promoter

   T7

   Available Since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. T2A 3xNLS GFP

   Plasmid

   #227721

   Purpose

   Plasmid contains T2A 3xNLS GFP, with some nlp-51 homology sequence before, Amp Resistant

   Depositor

   Haosheng Sun, Oliver Hobert

   Article

   Sun et al MicroPubl Biol. 2023 Nov 2;2023:10.17912

   Insert

   T2A 3xNLS GFP with some nlp-51 homology sequence (nlp-51 Nematode)
   

   Use

   Cloning vector

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRvTUB-mEGFP-NLS

    Plasmid

    #205011

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19/NLS-mChe

    Plasmid

    #183162

    Purpose

    Construct for transient expression of cell nucleus-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    NLS-mCherry
    

    Tags

    16 aa nuclear localization signal and 16 aa nucl…

    Expression

    Plant

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-nls-sfGFP

    Plasmid

    #132973

    Purpose

    full-length of nuclear localized sfGFP with stop codon in pmE Gateway vector

    Depositor

    Nathan Lawson

    Article

    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

    Insert

    nuclear localized super folder GFP
    

    Use

    Gateway vector

    Tags

    6x myc and SV40 NLS

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Construct 32 - NFKBRp_mKate2_2xNLS_p2a-puroR

    Plasmid

    #82024

    Purpose

    Lentiviral construct for building stable cell lines expressing mKate2 in the presence of TNFa via puromycin selection

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    mKate2-2xNLS
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLew100::NLS-ISceI-HA

    Plasmid

    #21299

    Depositor

    Nina Papavasiliou

    Article

    Boothroyd et al Nature. 2009 May 14;459(7244):278-81. do

    Insert

    I-SceI (SCEI )
    

    Tags

    HA and NLS

    Expression

    Mammalian

    Available Since

    Dec. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRvSAHS1-mEGFP-NLS

    Plasmid

    #205005

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRvCAHS3-mEGFP-NLS

    Plasmid

    #205004

    Purpose

    The vector contains 1kbp of the upstream and downstream regions of the cahs3 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

    Depositor

    Kazuharu Arakawa

    Article

    Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

    Insert

    mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs3 gene from Ramazzottius varieornatus
    

    Use

    Tardigrade expression

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. V5-LOV-Turbo-NLS

    Plasmid

    #199663

    Purpose

    expresses LOV-Turbo in the mammalian nucleus, lentiviral vector

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Lentiviral

    Tags

    NLS and V5

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP

    Plasmid

    #32627

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    Donor + Sce BFP
    

    Use

    Lentiviral

    Available Since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNLS-TetR-GFP

    Plasmid

    #103838

    Purpose

    Flourescent marker for tetO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    NLS-TetR (tetR )
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    TetR: A4G (M2V)

    Promoter

    CMV

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. epB_UbC_CymRV5-nls-Halox2_DEx4

    Plasmid

    #119907

    Purpose

    ePiggyBac mammalian expression plasmid for CymR-Halo fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    CymRV5-nls-Halox2
    

    Expression

    Mammalian

    Available Since

    April 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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