We narrowed to 6,260 results for: pcDNA 3.1

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pcDNA3.1 puro Nodamura B2

Plasmid

#17228

Depositor

Christopher Sullivan

Article

Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

Insert

Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)

Expression

Mammalian

Mutation

2 silent point mutations

Available Since

June 22, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1(+)-OTI-TCRA_p2_TCRB

Plasmid

#131033

Purpose

Expresses OT-I TCRA linked to TCRB with the P2A self-cleaving peptide

Depositor

Jeff Hammerbacher

Article

Aksoy et al bioRxiv

Inserts

TCRA (Tcra Mouse)
TCRB (Tcrb Mouse)

Expression

Mammalian

Promoter

CMV/T7

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Clover-mRuby2

Plasmid

#49089

Purpose

Clover-mRuby2 tandem fusion for FRET calibration

Depositor

Kurt Beam

Insert

Clover-mRuby2 fusion

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Peredox-mCherry

Plasmid

#32383

Depositor

Gary Yellen

Article

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+-Tornado-nLuc

Plasmid

#212611

Purpose

For expression of a circular nLuc mRNA. To express your gene of interest clone into the BsiWI, SacII sites and add the GCCATGTcTATGTGG 5' of the SacII site.

Depositor

Samie Jaffrey

Article

Unti et al Cell Chem Biol. 2023 Oct 13:S2451-9456(2

Insert

Tornado-CVB3-nLuc

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TYRO3-V5/HIS

Plasmid

#200118

Purpose

Expression of human TYRO3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human TYRO3 receptor tyrosine kinase (TYRO3 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hPTGS2-2flag

Plasmid

#102498

Purpose

Mammalian expression of PTGS2

Depositor

Jun Yu

Article

Chen et al Oncogene. 2017 Aug;36(31):4415-4426. doi

Insert

PTGS2 (PTGS2 Human)

Tags

Flag-Flag

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-1a

Plasmid

#26801

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

SREBP-1a (SREBF1 Human)

Tags

2xFLAG

Expression

Mammalian

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hSTING (R232)

Plasmid

#214152

Purpose

Transient expression of STING R232

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (STING1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-FLAG-SF3B1-WT

Plasmid

#82576

Purpose

Expresses a codon-optimized ORF of human SF3B1 (wild-type)

Depositor

Manoj Pillai

Article

Kesarwani et al Oncogene. 2016 Aug 15. doi: 10.1038/onc.

Insert

human SF3B1-WT (SF3B1 Human)

Tags

FLAG peptide

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-2xFLAG-SREBP-2

Plasmid

#26807

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)

Tags

2x FLAG

Expression

Mammalian

Mutation

L7V, G76S, K395R and I482L

Promoter

CMV

Available Since

July 26, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FGFR1c-V5/HIS

Plasmid

#201106

Purpose

expression of human FGFR1 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human FGFR1 receptor tyrosine kinase, variant c, full length, wildtype (FGFR1 Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VEGFR2-V5/HIS

Plasmid

#205617

Purpose

Expression of human VEGFR2 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

VEGFR2 receptor tyrosine kinase (KDR Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-ICUE

Plasmid

#181845

Purpose

Genetically encoded FRET-based sensor for monitoring cAMP dynamics near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

FluoSTEP-ICUE (RAPGEF3 Human)

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-AKAR

Plasmid

#181844

Purpose

Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

FluoSTEP-AKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GGamma2-GFP2

Plasmid

#166774

Purpose

Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

Depositor

Bryan Roth

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Insert

GGamma2-GFP2 (GNG2 Synthetic, Human)

Expression

Mammalian

Mutation

Contains an N-terminal GFP2 and GSAG linker

Promoter

CMV

Available Since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pY016 (pcDNA3.1-hLbCpf1)

Plasmid

#69988

Purpose

Expresses humanized LbCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(−) mCER-Prx2

Plasmid

#158224

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for transient transfection in mammalian cells)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-VEGFR2-mCherry

Plasmid

#139606

Purpose

Expresses a VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-KIT-V5/HIS

Plasmid

#201986

Purpose

expression of human KIT receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

KIT receptor tyrosine kinase (KIT Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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