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Showing: 8971 - 9000 of 22610 results

  4. pZH520

    Plasmid
    #102673
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102673-plasmid-map-sequence-id-199588

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  10. pZH521

    Plasmid
    #102674
    Purpose
    Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102674-plasmid-map-sequence-id-199585

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  11. pMito-dCherry-FRB

    Plasmid
    #186573
    Purpose
    Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells
    Depositor
    Stephen Royle
    Article
    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:
    Insert
    Dark MitoTrap
    Expression
    Mammalian
    Mutation
    Mutation of "K70N" to render mCherry no…
    Promoter
    CMV
    Available Since
    Aug. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    186573-plasmid-map-sequence-id-368442

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  13. pCytERM-LSSmGFP

    Plasmid
    #186540
    Purpose
    Visualization of the ER in cells using LSSmGFP (ex/em = 400/510 nm); compatible with OSER assay.
    Depositor
    Gregory Petsko
    Article
    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4
    Insert
    LSSmGFP
    Tags
    Cytochrome p450 first 29 animo acids (Oryctolagus…
    Expression
    Mammalian
    Mutation
    hfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206K
    Promoter
    CMV
    Available Since
    Nov. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186540-plasmid-map-sequence-id-368221

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  19. pZH517

    Plasmid
    #102670
    Purpose
    Tetracycline inducible expression of GFPmut2 using autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly
    Depositor
    Zach Hensel
    Article
    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d
    Insert
    GFPmut2
    Expression
    Bacterial
    Available Since
    Dec. 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    102670-plasmid-map-sequence-id-199582

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  23. pTJV1Sc2-tetA

    Plasmid
    #166982
    Purpose
    Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    CspRecT
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    pVanCC
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166982-plasmid-map-sequence-id-331335

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Showing: 8971 - 9000 of 22610 results