We narrowed to 8,781 results for: construct
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Plasmid#133300PurposeThe puromycin selection marker was replaced by blasticidin in SHC003. This construct could be used together with other plasmid contains puromycin selection marker.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only
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pENTR-L1-PGK-TKBsd-L2
Plasmid#141009PurposeMutisite Gateway mediated vector constructionDepositorInsertTKBsd
ExpressionMammalianAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a_TauFRET_3xKQ_wpre
Plasmid#207522PurposeLentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cellsDepositorInsertTauFRET_P301L_3xKQ
UseLentiviralTagsNeonGreen and mTurquoise2MutationP301L; K311Q; K317Q; K321QPromoterEF1aAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1_HyR-Ck1
Plasmid#136125PurposeL1 in position 1 (next to LB in L2 construct), for HygRDepositorInsertp5-35S:hptII_3t35S
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSO7
Plasmid#106365PurposeTo insert a single copy of a construct at a site on Chr I, corresponding to the Shigella flexneri protein OspF (a MAP kinase inhibitor) expressed specifically in the epidermis.DepositorInsertttTi4348_ SEC _pcol-19_OspF_3'unc54UTR
ExpressionWormAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSicoR
Plasmid#11579PurposeCre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF shRNA expression.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiExpressionMammalianAvailable SinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet3-Peroxi_C1
Plasmid#189769PurposeConstruct for labelling peroxisomes in mammalian cells with peroxisomal targeted mScarlet3DepositorInsertmScarlet3-Peroxi
ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Fragment1 with TLS2
Plasmid#196983PurposeGateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.DepositorInsertIntermediate fragment for assembly of gRNA-TLS2 construct
UseGateway-compatible entry vectorAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSico PGK puro
Plasmid#11586PurposeCre-regulated lentiviral shRNA vector. Cre addition causes the puromycin gene to be recombined out of the construct, activating shRNA expression.DepositorTypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiExpressionMammalianAvailable SinceJuly 19, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro_shTBK1_3185
Plasmid#167294PurposeLentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.DepositorAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-FKBP:mCherry-Rab7a
Plasmid#72903PurposeLate endosome-targeted mCherry-tagged recruiter constructDepositorAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-FKBP:mCherry-Rab11a
Plasmid#72902PurposeRecycling endosome-targeted mCherry-tagged recruiter constructDepositorInsertRab11a (RAB11A Human)
UseLentiviralTagsFKBP and mCherryExpressionMammalianMutationfragment a.a. 2-216Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
MGS0156_pET29b
Plasmid#215410PurposeExpression construct for MGS0156 gene, codon optimized for expression in E. coliDepositorInsertMGS0156
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNG1299
Plasmid#149709PurposeTemplate for Malachite Green Aptamer (MGA) transcription assayDepositorInsertTriple Promoter - 12X MG Aptamer Array - Terminator construct
UseSynthetic BiologyAvailable SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
BA31_pU.CAG.SaCas9-D10A.rBGpA
Plasmid#199251PurposeExpression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)DepositorInsertSaCas9.D10A nickase
UseCRISPRTags3XHA epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationD10APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-mTagBFP
Plasmid#75148PurposeTagBFP (blue) fluorophore for use with 5’ promoters.DepositorInsertmTagBFP
UseMiddle entry vector for multisite gateway three f…Promotern/aAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5E-mfap4
Plasmid#70052Purpose5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.DepositorInsertmfap4 promoter
UseGateway 5' elementAvailable SinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro Non-targeting sgRNA 1
Plasmid#109002PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #1
UseLentiviralExpressionMammalianPromotermU6 promoterAvailable SinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
human-GFAP-EGFP
Plasmid#169473PurposeWe generated a human-GFAP-EGFP fusion construct by randomly inserting the coding region of EFGP into the coding region of hGFAP.DepositorAvailable SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_sg30-PDGFR_EF1a_mCherry
Plasmid#186309PurposePlasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line constructionDepositorInsertssg30-PDGFR
mCherry
TagsHis, mycExpressionMammalianAvailable SinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only