We narrowed to 22,553 results for: promoter

1. pGP18 (CK2alpha' K69M)

   Plasmid

   #27090

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2alpha' K69M (CSNK2A2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K69M

   Promoter

   CMV

   Available since

   Oct. 14, 2011

   Availability

   Academic Institutions and Nonprofits only

2. CDK4-luciferase full length (2135)

   Plasmid

   #86656

   Purpose

   Luciferase reporter containing CDK4 promoter

   Depositor

   Jaime Modiano

   Article

   Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

   Insert

   CDK4 promoter (CDK4 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   2120 base pairs including up to coding nucleotide…

   Promoter

   CDK4

   Available since

   June 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. NSP3C-Flag

   Plasmid

   #210349

   Purpose

   Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   C terminal of SARS-CoV-2 NSP3 (ORF1ab )
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEF073 MBP-Axin-His

   Plasmid

   #154011

   Purpose

   Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli

   Depositor

   Jesse Zalatan

   Article

   Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

   Insert

   Axin1 (AXIN1 Human)
   

   Use

   Tags

   His6 and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3-myc3-FBW5 delta F

   Plasmid

   #19912

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   F-box and WD repeat domain containing 5 delta F (FBXW5 Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   deleted aa1-42 (F-box)

   Promoter

   Available since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAB07 (CK2beta)

   Plasmid

   #27091

   Depositor

   David Litchfield

   Article

   Turowec et al Methods Enzymol. 2010;484:471-93.

   Insert

   CK2beta (CSNK2B Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

7. EGFP in pDBEcR-pIND(SP1)-DD-W/Bla

   Plasmid

   #201560

   Purpose

   Ecdysone-inducible vector with SB ITRs for expressing FLAG- EGFP-DD (WPRE in 3' UTR) and DBEcR (3xmyc-tagged); blasticidin resistance.

   Depositor

   Randy Poon

   Article

   Yeung et al J Cell Sci. 2023 Apr 15;136(8):jcs260754

   Insert

   DBEcR and EGFP
   

   Use

   Unspecified; Sleeping beauty transposon

   Tags

   DBEcR-myc, FLAG-EGFP, and FLAG-EGFP-DD

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and pIND(SP1)

   Available since

   July 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDEST-Tol2(Chien)-Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA

   Plasmid

   #105643

   Purpose

   Co-expression of tdTomato and HyPer in differentiated keratinocytes of the periderm layer and ealy on also in the basal layer of the epidermis

   Depositor

   Sandra Rieger

   Article

   Lisse et al Proc Natl Acad Sci U S A. 2016 Apr 12;11

   Insert

   Krt4:Gal4VP16:tdTomato:5xUAS:HyPer:pA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

9. pMSCV-SMYD2 F184A

   Plasmid

   #85659

   Purpose

   retroviral expression of human SMYD2 F184A

   Depositor

   Julien Sage

   Article

   Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

   Insert

   SMYD2 (SMYD2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   F184A

   Promoter

   LTR

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-T7-hOsa1

    Plasmid

    #17986

    Depositor

    Naoko Tanese

    Article

    Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

    Insert

    Osa1 (ARID1A Human)
    

    Use

    Tags

    T7 epitope

    Expression

    Mammalian

    Mutation

    Missing 5' end

    Promoter

    Available since

    Aug. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

11. AYBEv0.1

    Plasmid

    #193966

    Purpose

    A wildtype adenine transversion base editor (AYBEv0.1, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    ecTadA8e-nSpCas9(D10A)-MPG(wt)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF1a, CBh

    Available since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR RPS27A K107/113R-3xFlag_IRES-GFP

    Plasmid

    #198392

    Purpose

    RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

    Depositor

    Jack Taunton

    Article

    Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

    Insert

    RPS27A (RPS27A Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    ubiquitination site double mutant (K107/113R)

    Promoter

    EF1A

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDN-D2irC6kwh

    Plasmid

    #44725

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Nat Commun. 2013 Feb 5;4:1451. doi: 10.1

    Inserts

    pCMV-D2i promoter
    mCherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Rabbit β-globin intron II and WPRE

    Expression

    Mammalian

    Mutation

    Initiator motif (Inr) displaced relative to pCMV-…

    Promoter

    pCMV-D2i

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL4.24-Cd36-enhancer4

    Plasmid

    #138576

    Purpose

    Firefly luciferase enhancer reporter fused with 1.5~2 kb fragments of mouse Cd36 enhancers at Chr5:17876439_17877752

    Depositor

    Lora Hooper

    Article

    Kuang et al Science. 2019 Sep 27;365(6460):1428-1434

    Insert

    Cd36 Promoter (Cd36 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Minimal promoter

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124542

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. psiCHECK-2-TGFBR2-wt_3'UTR

    Plasmid

    #31882

    Depositor

    Robert Blelloch

    Article

    Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

    Insert

    TGFBR2 wild-type 3'UTR (TGFBR2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pgRGFP

    Plasmid

    #82695

    Purpose

    Expresses gRNA of interest under U6 promoter (cloning by BpiI) and GFP under PGK promoter.

    Depositor

    Alan Mullen

    Article

    Daneshvar et al Cell Rep. 2016 Oct 4;17(2):353-365. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK, U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFAB3833

    Plasmid

    #47839

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 8 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 8 and BCD1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    see insert

    Available since

    Dec. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

    Plasmid

    #124543

    Purpose

    Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41

    Promoter

    Minimal ADE

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFAB3737

    Plasmid

    #47838

    Purpose

    BIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.

    Depositor

    Drew Endy

    Article

    Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

    Insert

    promoter 7 and BCD1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    see insert

    Available since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge